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Acoustofluidics 11: Affinity specific extraction and sample decomplexing using continuous flow acoustophoresis.

Augustsson, Per LU and Laurell, Thomas LU (2012) In Lab on A Chip 12(10). p.1742-1752
Abstract
Acoustic standing wave technology combined with ligand complexed microbeads offers a means for affinity specific selection of target analytes from complex samples. When realized in a microfluidic format we can capitalize on laminar flow and acoustic forces that can drive cells or microbeads across fluid interfaces. Given this, we have the ability to perform carrier fluid (suspending medium) exchange operations in continuous flow in microfluidic chips based solely on acoustofluidic properties. A key issue here is to ensure that a minimum of the original carrier fluid follows the cells/particles across the fluid interface. Simple processing protocols can be achieved that may outperform macroscale magnetic bead-based sample extraction or... (More)
Acoustic standing wave technology combined with ligand complexed microbeads offers a means for affinity specific selection of target analytes from complex samples. When realized in a microfluidic format we can capitalize on laminar flow and acoustic forces that can drive cells or microbeads across fluid interfaces. Given this, we have the ability to perform carrier fluid (suspending medium) exchange operations in continuous flow in microfluidic chips based solely on acoustofluidic properties. A key issue here is to ensure that a minimum of the original carrier fluid follows the cells/particles across the fluid interface. Simple processing protocols can be achieved that may outperform macroscale magnetic bead-based sample extraction or centrifugation steps, which can also be straightforwardly integrated with downstream analytical instrumentation. This tutorial outlines some basic fluidic configurations for acoustophoresis based sample decomplexing and details the different system parameters that will impact the outcome of an acoustophoresis based affinity extraction experiment or a cell medium exchange step. Examples are given of both targeted extraction of microbes and selective elusion of molecular species. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Lab on A Chip
volume
12
issue
10
pages
1742 - 1752
publisher
Royal Society of Chemistry
external identifiers
  • pmid:22465997
  • wos:000303167200002
  • scopus:84860367830
ISSN
1473-0189
DOI
10.1039/c2lc40200a
language
English
LU publication?
yes
id
9d7367f1-edfc-4381-999b-77d0653441de (old id 2520007)
date added to LUP
2012-05-09 14:34:59
date last changed
2017-10-01 03:06:04
@article{9d7367f1-edfc-4381-999b-77d0653441de,
  abstract     = {Acoustic standing wave technology combined with ligand complexed microbeads offers a means for affinity specific selection of target analytes from complex samples. When realized in a microfluidic format we can capitalize on laminar flow and acoustic forces that can drive cells or microbeads across fluid interfaces. Given this, we have the ability to perform carrier fluid (suspending medium) exchange operations in continuous flow in microfluidic chips based solely on acoustofluidic properties. A key issue here is to ensure that a minimum of the original carrier fluid follows the cells/particles across the fluid interface. Simple processing protocols can be achieved that may outperform macroscale magnetic bead-based sample extraction or centrifugation steps, which can also be straightforwardly integrated with downstream analytical instrumentation. This tutorial outlines some basic fluidic configurations for acoustophoresis based sample decomplexing and details the different system parameters that will impact the outcome of an acoustophoresis based affinity extraction experiment or a cell medium exchange step. Examples are given of both targeted extraction of microbes and selective elusion of molecular species.},
  author       = {Augustsson, Per and Laurell, Thomas},
  issn         = {1473-0189},
  language     = {eng},
  number       = {10},
  pages        = {1742--1752},
  publisher    = {Royal Society of Chemistry},
  series       = {Lab on A Chip},
  title        = {Acoustofluidics 11: Affinity specific extraction and sample decomplexing using continuous flow acoustophoresis.},
  url          = {http://dx.doi.org/10.1039/c2lc40200a},
  volume       = {12},
  year         = {2012},
}