Identification of a novel protein kinase Cδ-Smac complex that dissociates during paclitaxel-induced cell death.
(2012) In FEBS Letters 586(8). p.1166-1172- Abstract
- Protein kinase C (PKC) δ is a regulator of apoptosis with both pro- and anti-apoptotic effects. The mechanistic basis for the discrepant effects is not completely understood. Here we show that Smac interacts with PKCδ. The interaction depends on the N-terminus of Smac and is disrupted upon treatment with paclitaxel. This is associated with release of Smac into the cytosol. Activation of PKCδ rescues the interaction during paclitaxel exposure and suppresses the paclitaxel-mediated cell death. However, under these conditions the complex is mainly found in the cytosol suggesting that cytosolic Smac can be bound by PKCδ when PKC is activated. The data unravel a previously unrecognized interaction and suggest that PKCδ by associating with Smac... (More)
- Protein kinase C (PKC) δ is a regulator of apoptosis with both pro- and anti-apoptotic effects. The mechanistic basis for the discrepant effects is not completely understood. Here we show that Smac interacts with PKCδ. The interaction depends on the N-terminus of Smac and is disrupted upon treatment with paclitaxel. This is associated with release of Smac into the cytosol. Activation of PKCδ rescues the interaction during paclitaxel exposure and suppresses the paclitaxel-mediated cell death. However, under these conditions the complex is mainly found in the cytosol suggesting that cytosolic Smac can be bound by PKCδ when PKC is activated. The data unravel a previously unrecognized interaction and suggest that PKCδ by associating with Smac may prevent its apoptotic effects. STRUCTURED SUMMARY OF PROTEIN INTERACTIONS: PKC deltaphysically interacts with SMAC by anti bait coimmunoprecipitation (View Interaction: 1, 2, 3, 4) XIAPphysically interacts with SMAC by anti tag coimmunoprecipitation(View interaction). (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2520012
- author
- Masoumi, Katarzyna LU ; Cornmark, Louise LU ; Kalstad Lönne, Gry LU ; Hellman, Ulf and Larsson, Christer LU
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Smac, Protein kinase C, Apoptosis, Cell death, Paclitaxel, XIAP
- in
- FEBS Letters
- volume
- 586
- issue
- 8
- pages
- 1166 - 1172
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000303106100013
- pmid:22465666
- scopus:84859898683
- pmid:22465666
- ISSN
- 1873-3468
- DOI
- 10.1016/j.febslet.2012.03.033
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Tumour Cell Biology (013017530)
- id
- 2ca9d9a1-0d43-460c-8ecd-091f69053908 (old id 2520012)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/22465666?dopt=Abstract
- date added to LUP
- 2016-04-01 14:21:45
- date last changed
- 2022-01-28 00:13:10
@article{2ca9d9a1-0d43-460c-8ecd-091f69053908, abstract = {{Protein kinase C (PKC) δ is a regulator of apoptosis with both pro- and anti-apoptotic effects. The mechanistic basis for the discrepant effects is not completely understood. Here we show that Smac interacts with PKCδ. The interaction depends on the N-terminus of Smac and is disrupted upon treatment with paclitaxel. This is associated with release of Smac into the cytosol. Activation of PKCδ rescues the interaction during paclitaxel exposure and suppresses the paclitaxel-mediated cell death. However, under these conditions the complex is mainly found in the cytosol suggesting that cytosolic Smac can be bound by PKCδ when PKC is activated. The data unravel a previously unrecognized interaction and suggest that PKCδ by associating with Smac may prevent its apoptotic effects. STRUCTURED SUMMARY OF PROTEIN INTERACTIONS: PKC deltaphysically interacts with SMAC by anti bait coimmunoprecipitation (View Interaction: 1, 2, 3, 4) XIAPphysically interacts with SMAC by anti tag coimmunoprecipitation(View interaction).}}, author = {{Masoumi, Katarzyna and Cornmark, Louise and Kalstad Lönne, Gry and Hellman, Ulf and Larsson, Christer}}, issn = {{1873-3468}}, keywords = {{Smac; Protein kinase C; Apoptosis; Cell death; Paclitaxel; XIAP}}, language = {{eng}}, number = {{8}}, pages = {{1166--1172}}, publisher = {{Wiley-Blackwell}}, series = {{FEBS Letters}}, title = {{Identification of a novel protein kinase Cδ-Smac complex that dissociates during paclitaxel-induced cell death.}}, url = {{http://dx.doi.org/10.1016/j.febslet.2012.03.033}}, doi = {{10.1016/j.febslet.2012.03.033}}, volume = {{586}}, year = {{2012}}, }