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Identification of a novel protein kinase Cδ-Smac complex that dissociates during paclitaxel-induced cell death.

Masoumi, Katarzyna LU ; Cornmark, Louise LU ; Kalstad Lönne, Gry LU ; Hellman, Ulf and Larsson, Christer LU (2012) In FEBS Letters 586(8). p.1166-1172
Abstract
Protein kinase C (PKC) δ is a regulator of apoptosis with both pro- and anti-apoptotic effects. The mechanistic basis for the discrepant effects is not completely understood. Here we show that Smac interacts with PKCδ. The interaction depends on the N-terminus of Smac and is disrupted upon treatment with paclitaxel. This is associated with release of Smac into the cytosol. Activation of PKCδ rescues the interaction during paclitaxel exposure and suppresses the paclitaxel-mediated cell death. However, under these conditions the complex is mainly found in the cytosol suggesting that cytosolic Smac can be bound by PKCδ when PKC is activated. The data unravel a previously unrecognized interaction and suggest that PKCδ by associating with Smac... (More)
Protein kinase C (PKC) δ is a regulator of apoptosis with both pro- and anti-apoptotic effects. The mechanistic basis for the discrepant effects is not completely understood. Here we show that Smac interacts with PKCδ. The interaction depends on the N-terminus of Smac and is disrupted upon treatment with paclitaxel. This is associated with release of Smac into the cytosol. Activation of PKCδ rescues the interaction during paclitaxel exposure and suppresses the paclitaxel-mediated cell death. However, under these conditions the complex is mainly found in the cytosol suggesting that cytosolic Smac can be bound by PKCδ when PKC is activated. The data unravel a previously unrecognized interaction and suggest that PKCδ by associating with Smac may prevent its apoptotic effects. STRUCTURED SUMMARY OF PROTEIN INTERACTIONS: PKC deltaphysically interacts with SMAC by anti bait coimmunoprecipitation (View Interaction: 1, 2, 3, 4) XIAPphysically interacts with SMAC by anti tag coimmunoprecipitation(View interaction). (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Smac, Protein kinase C, Apoptosis, Cell death, Paclitaxel, XIAP
in
FEBS Letters
volume
586
issue
8
pages
1166 - 1172
publisher
Wiley-Blackwell
external identifiers
  • wos:000303106100013
  • pmid:22465666
  • scopus:84859898683
ISSN
1873-3468
DOI
10.1016/j.febslet.2012.03.033
language
English
LU publication?
yes
id
2ca9d9a1-0d43-460c-8ecd-091f69053908 (old id 2520012)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/22465666?dopt=Abstract
date added to LUP
2012-05-04 13:16:30
date last changed
2017-07-02 04:06:11
@article{2ca9d9a1-0d43-460c-8ecd-091f69053908,
  abstract     = {Protein kinase C (PKC) δ is a regulator of apoptosis with both pro- and anti-apoptotic effects. The mechanistic basis for the discrepant effects is not completely understood. Here we show that Smac interacts with PKCδ. The interaction depends on the N-terminus of Smac and is disrupted upon treatment with paclitaxel. This is associated with release of Smac into the cytosol. Activation of PKCδ rescues the interaction during paclitaxel exposure and suppresses the paclitaxel-mediated cell death. However, under these conditions the complex is mainly found in the cytosol suggesting that cytosolic Smac can be bound by PKCδ when PKC is activated. The data unravel a previously unrecognized interaction and suggest that PKCδ by associating with Smac may prevent its apoptotic effects. STRUCTURED SUMMARY OF PROTEIN INTERACTIONS: PKC deltaphysically interacts with SMAC by anti bait coimmunoprecipitation (View Interaction: 1, 2, 3, 4) XIAPphysically interacts with SMAC by anti tag coimmunoprecipitation(View interaction).},
  author       = {Masoumi, Katarzyna and Cornmark, Louise and Kalstad Lönne, Gry and Hellman, Ulf and Larsson, Christer},
  issn         = {1873-3468},
  keyword      = {Smac,Protein kinase C,Apoptosis,Cell death,Paclitaxel,XIAP},
  language     = {eng},
  number       = {8},
  pages        = {1166--1172},
  publisher    = {Wiley-Blackwell},
  series       = {FEBS Letters},
  title        = {Identification of a novel protein kinase Cδ-Smac complex that dissociates during paclitaxel-induced cell death.},
  url          = {http://dx.doi.org/10.1016/j.febslet.2012.03.033},
  volume       = {586},
  year         = {2012},
}