Advanced

Human immunodeficiency virus type 1 vectors with alphavirus envelope glycoproteins produced from stable packaging cells

Strang, BL; Takeuchi, Y; Relander, Thomas LU ; Richter, Johan LU ; Bailey, R; Sanders, DA; Collins, MKL and Ikeda, Y (2005) In Journal of Virology 79(3). p.1765-1771
Abstract
Alphavirus glycoproteins have broad host ranges. Human immunodeficiency virus type 1 (HIV-1) vectors pseudotyped with their glycoproteins could extend the range of tissues that can be transduced in both humans and animal models. Here, we established stable producer cell lines for HIV vectors pseudotyped with alphavirus Ross River virus (RRV) and Semliki Forest virus (SFV) glycoproteins E2E1. RRV E2E1-stable clones could routinely produce high-titer pseudotyped vectors for at least 5 months. SFV E2E1-stable clones, however, produced relatively low titers. We examined the properties of RRV E2E1-pseudotyped vectors [HIV-1(RRV)] and compared them with amphotropic murine leukemia virus Env- and vesicular stomatitis virus glycoprotein... (More)
Alphavirus glycoproteins have broad host ranges. Human immunodeficiency virus type 1 (HIV-1) vectors pseudotyped with their glycoproteins could extend the range of tissues that can be transduced in both humans and animal models. Here, we established stable producer cell lines for HIV vectors pseudotyped with alphavirus Ross River virus (RRV) and Semliki Forest virus (SFV) glycoproteins E2E1. RRV E2E1-stable clones could routinely produce high-titer pseudotyped vectors for at least 5 months. SFV E2E1-stable clones, however, produced relatively low titers. We examined the properties of RRV E2E1-pseudotyped vectors [HIV-1(RRV)] and compared them with amphotropic murine leukemia virus Env- and vesicular stomatitis virus glycoprotein G-pseudotyped vectors. HrV-1 (RRV) displayed a number of characteristics which would be advantageous in ex vivo and in vivo experiments, including resistance to inactivation by heat-labile components in fresh human sera and thermostability at 37degreesC. Upon single-step concentration by ultracentrifugation of HIV-1(RRV), we could achieve vector stocks with titers up to 6 x 10(7) IU/ml. HIV-1(RRV) efficiently transduced cells from several different species, including murine primary dendritic cells, but failed to transduce human and murine T cells as well as human hematopoietic stem cells (HSC). These results indicate that HIV-1(RRV) could be used in a number of applications including animal model experiments and suggest that expression of RRV cellular receptors is limited or absent in certain cell types such as T cells and human HSC. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Virology
volume
79
issue
3
pages
1765 - 1771
publisher
American Society for Microbiology
external identifiers
  • pmid:15650201
  • wos:000226634300042
  • scopus:13744261478
ISSN
1098-5514
DOI
10.1128/JVI.79.3.1765-1771.2005
language
English
LU publication?
yes
id
77d58336-ce4d-435b-a873-7d748064057a (old id 254762)
date added to LUP
2007-08-14 15:49:20
date last changed
2017-02-12 04:10:37
@article{77d58336-ce4d-435b-a873-7d748064057a,
  abstract     = {Alphavirus glycoproteins have broad host ranges. Human immunodeficiency virus type 1 (HIV-1) vectors pseudotyped with their glycoproteins could extend the range of tissues that can be transduced in both humans and animal models. Here, we established stable producer cell lines for HIV vectors pseudotyped with alphavirus Ross River virus (RRV) and Semliki Forest virus (SFV) glycoproteins E2E1. RRV E2E1-stable clones could routinely produce high-titer pseudotyped vectors for at least 5 months. SFV E2E1-stable clones, however, produced relatively low titers. We examined the properties of RRV E2E1-pseudotyped vectors [HIV-1(RRV)] and compared them with amphotropic murine leukemia virus Env- and vesicular stomatitis virus glycoprotein G-pseudotyped vectors. HrV-1 (RRV) displayed a number of characteristics which would be advantageous in ex vivo and in vivo experiments, including resistance to inactivation by heat-labile components in fresh human sera and thermostability at 37degreesC. Upon single-step concentration by ultracentrifugation of HIV-1(RRV), we could achieve vector stocks with titers up to 6 x 10(7) IU/ml. HIV-1(RRV) efficiently transduced cells from several different species, including murine primary dendritic cells, but failed to transduce human and murine T cells as well as human hematopoietic stem cells (HSC). These results indicate that HIV-1(RRV) could be used in a number of applications including animal model experiments and suggest that expression of RRV cellular receptors is limited or absent in certain cell types such as T cells and human HSC.},
  author       = {Strang, BL and Takeuchi, Y and Relander, Thomas and Richter, Johan and Bailey, R and Sanders, DA and Collins, MKL and Ikeda, Y},
  issn         = {1098-5514},
  language     = {eng},
  number       = {3},
  pages        = {1765--1771},
  publisher    = {American Society for Microbiology},
  series       = {Journal of Virology},
  title        = {Human immunodeficiency virus type 1 vectors with alphavirus envelope glycoproteins produced from stable packaging cells},
  url          = {http://dx.doi.org/10.1128/JVI.79.3.1765-1771.2005},
  volume       = {79},
  year         = {2005},
}