Advanced

Archaeal abundance in relation to root and fungal exudation rates

Sterngren, Anna LU ; Johansson, Tomas LU and Bengtson, Per LU (2012) In FEMS Microbiology Ecology 80(2). p.305-311
Abstract
Archaea are ubiquitous in forest soils, but little is known about the factors regulating their abundance and distribution. Low molecular weight organic compounds represent an important energy source for archaea in marine environments, and it is reasonable to suspect that archaeal abundance is dependent on such compounds in soils as well, represented by, for example, plant and fungal exudates. To test this hypothesis, we designed a microcosm experiment in which we grew ponderosa pine, sitka spruce, and western hemlock in forest soil. Root and mycorrhizal exudation rates were estimated in a 13C pulse-chase experiment, and the number of archaeal and bacterial 16S rRNA genes was determined by qPCR. Archaeal abundance differed among plant... (More)
Archaea are ubiquitous in forest soils, but little is known about the factors regulating their abundance and distribution. Low molecular weight organic compounds represent an important energy source for archaea in marine environments, and it is reasonable to suspect that archaeal abundance is dependent on such compounds in soils as well, represented by, for example, plant and fungal exudates. To test this hypothesis, we designed a microcosm experiment in which we grew ponderosa pine, sitka spruce, and western hemlock in forest soil. Root and mycorrhizal exudation rates were estimated in a 13C pulse-chase experiment, and the number of archaeal and bacterial 16S rRNA genes was determined by qPCR. Archaeal abundance differed among plant species, and the number of archaeal 16S rRNA genes was generally lower in soil receiving high concentration of exudates. The mycorrhizal fungi of ponderosa pine seemed to favor archaea, while no such effect was found for mycorrhized sitka spruce or western hemlock. The low abundance of archaea in the proximity of roots and mycorrhiza may be a result of slow growth rates and poor competitive ability of archaea vs. bacteria and does not necessarily reflect a lack of heterotrophic abilities of the archaeal community. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
archaea, bacteria, mycorrhiza, rhizosphere, root exudation, soil
in
FEMS Microbiology Ecology
volume
80
issue
2
pages
305 - 311
publisher
Elsevier
external identifiers
  • wos:000302610700004
  • scopus:84859590144
ISSN
1574-6941
DOI
10.1111/j.1574-6941.2012.01298.x
project
BECC
language
English
LU publication?
yes
id
6e7a15c4-8bc7-42c3-af8f-51fb3443b4e1 (old id 2571522)
date added to LUP
2012-06-04 08:18:30
date last changed
2017-05-21 04:06:58
@article{6e7a15c4-8bc7-42c3-af8f-51fb3443b4e1,
  abstract     = {Archaea are ubiquitous in forest soils, but little is known about the factors regulating their abundance and distribution. Low molecular weight organic compounds represent an important energy source for archaea in marine environments, and it is reasonable to suspect that archaeal abundance is dependent on such compounds in soils as well, represented by, for example, plant and fungal exudates. To test this hypothesis, we designed a microcosm experiment in which we grew ponderosa pine, sitka spruce, and western hemlock in forest soil. Root and mycorrhizal exudation rates were estimated in a 13C pulse-chase experiment, and the number of archaeal and bacterial 16S rRNA genes was determined by qPCR. Archaeal abundance differed among plant species, and the number of archaeal 16S rRNA genes was generally lower in soil receiving high concentration of exudates. The mycorrhizal fungi of ponderosa pine seemed to favor archaea, while no such effect was found for mycorrhized sitka spruce or western hemlock. The low abundance of archaea in the proximity of roots and mycorrhiza may be a result of slow growth rates and poor competitive ability of archaea vs. bacteria and does not necessarily reflect a lack of heterotrophic abilities of the archaeal community.},
  author       = {Sterngren, Anna and Johansson, Tomas and Bengtson, Per},
  issn         = {1574-6941},
  keyword      = {archaea,bacteria,mycorrhiza,rhizosphere,root exudation,soil},
  language     = {eng},
  number       = {2},
  pages        = {305--311},
  publisher    = {Elsevier},
  series       = {FEMS Microbiology Ecology},
  title        = {Archaeal abundance in relation to root and fungal exudation rates},
  url          = {http://dx.doi.org/10.1111/j.1574-6941.2012.01298.x},
  volume       = {80},
  year         = {2012},
}