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Tenocyte response to cyclical strain and transforming growth factor beta is dependent upon age and site of origin

Goodman, SA; May, SA; Heinegård, Dick LU and Smith, RKW (2004) In Biorheology 41(5). p.613-628
Abstract
The effect of strain and transforming growth factor beta on equine tendon fibroblasts (tenocytes) was assessed in vitro. Tenocytes were isolated from flexor and extensor tendons of horses from foetal to 10 years of age. These cells were cultured until confluent on collagen-coated silicone dishes. Cyclic biaxial strain of 9 +/- 1% was applied at 0.5 Hz for 24 hours with or without added TGFbeta1 or 3 (10 ng/ml). Proliferation and synthetic responses were dependent on the tendon of origin. Neither strain nor TGFbeta caused flexor tenocytes to proliferate significantly, while strain alone did proliferate extensor tenocytes. TGFbeta, with or without strain, increased the incorporation of [H-3]-proline and the production of types I and III... (More)
The effect of strain and transforming growth factor beta on equine tendon fibroblasts (tenocytes) was assessed in vitro. Tenocytes were isolated from flexor and extensor tendons of horses from foetal to 10 years of age. These cells were cultured until confluent on collagen-coated silicone dishes. Cyclic biaxial strain of 9 +/- 1% was applied at 0.5 Hz for 24 hours with or without added TGFbeta1 or 3 (10 ng/ml). Proliferation and synthetic responses were dependent on the tendon of origin. Neither strain nor TGFbeta caused flexor tenocytes to proliferate significantly, while strain alone did proliferate extensor tenocytes. TGFbeta, with or without strain, increased the incorporation of [H-3]-proline and the production of types I and III collagen and COMP in both cell types, although the effect on COMP production was more marked in flexor tenocytes, perhaps reflecting the higher levels found in this tendon in vivo. Immature flexor tenocytes synthesised more collagen and COMP than those from mature animals. while age had little effect in extensor tenocytes. Our results suggest that tenocytes become differentiated at an early age and present tendon-specific responses. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biorheology
volume
41
issue
5
pages
613 - 628
publisher
IOS Press
external identifiers
  • pmid:15477668
  • wos:000226305900002
  • scopus:16644377247
ISSN
0006-355X
language
English
LU publication?
yes
id
9e0d8543-e859-45bb-9e87-bdb0beafa720 (old id 257633)
alternative location
http://iospress.metapress.com/openurl.asp?genre=article&issn=0006-355X&volume=41&issue=5&spage=613
date added to LUP
2007-10-29 09:07:08
date last changed
2017-10-22 03:39:11
@article{9e0d8543-e859-45bb-9e87-bdb0beafa720,
  abstract     = {The effect of strain and transforming growth factor beta on equine tendon fibroblasts (tenocytes) was assessed in vitro. Tenocytes were isolated from flexor and extensor tendons of horses from foetal to 10 years of age. These cells were cultured until confluent on collagen-coated silicone dishes. Cyclic biaxial strain of 9 +/- 1% was applied at 0.5 Hz for 24 hours with or without added TGFbeta1 or 3 (10 ng/ml). Proliferation and synthetic responses were dependent on the tendon of origin. Neither strain nor TGFbeta caused flexor tenocytes to proliferate significantly, while strain alone did proliferate extensor tenocytes. TGFbeta, with or without strain, increased the incorporation of [H-3]-proline and the production of types I and III collagen and COMP in both cell types, although the effect on COMP production was more marked in flexor tenocytes, perhaps reflecting the higher levels found in this tendon in vivo. Immature flexor tenocytes synthesised more collagen and COMP than those from mature animals. while age had little effect in extensor tenocytes. Our results suggest that tenocytes become differentiated at an early age and present tendon-specific responses.},
  author       = {Goodman, SA and May, SA and Heinegård, Dick and Smith, RKW},
  issn         = {0006-355X},
  language     = {eng},
  number       = {5},
  pages        = {613--628},
  publisher    = {IOS Press},
  series       = {Biorheology},
  title        = {Tenocyte response to cyclical strain and transforming growth factor beta is dependent upon age and site of origin},
  volume       = {41},
  year         = {2004},
}