Advanced

Autonomous protein sample processing on-chip using solid-phase microextraction, capillary force pumping, and microdispensing

Wallman, Lars LU ; Ekström, Simon LU ; Marko-Varga, György LU ; Laurell, Thomas LU and Nilsson, Johan LU (2004) In Electrophoresis 25(21-22). p.3778-3787
Abstract
A capillary force filling microsystem consisting of a chip-integrated solid-phase microextraction (SMEC) array and a microdispenser for sample purification and trace enrichment of peptides is described. The microextraction array was loaded with solid-phase media (50 mum Poros R2 beads) for purification and enrichment of proteomic samples. Samples bound to the SMEC were eluted in a volume of 200 nL. A piezoelectric microdispenser was docked to the array and the samples bound to the SMEC were eluted in a volume of 200 nL using capillary forces. The purified and enriched samples were dispensed onto the matrix-assisted laser desorption/ionization (MALDI) target, providing quality data from samples in the picomolar range. The nanoproteomic... (More)
A capillary force filling microsystem consisting of a chip-integrated solid-phase microextraction (SMEC) array and a microdispenser for sample purification and trace enrichment of peptides is described. The microextraction array was loaded with solid-phase media (50 mum Poros R2 beads) for purification and enrichment of proteomic samples. Samples bound to the SMEC were eluted in a volume of 200 nL. A piezoelectric microdispenser was docked to the array and the samples bound to the SMEC were eluted in a volume of 200 nL using capillary forces. The purified and enriched samples were dispensed onto the matrix-assisted laser desorption/ionization (MALDI) target, providing quality data from samples in the picomolar range. The nanoproteomic platform was compared to corresponding commercial preparation protocols, showing higher mass spectrometry (MS) signal intensities for peptides generated from an alpha-casein digest. The platform was also elvaluated with regards to two-dimensional (2-D) gel-derived protein digests from both fibroblast and epithelial target cells. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
microextraction, miniaturization, mass spectrometry, solid-phase, proteomics
in
Electrophoresis
volume
25
issue
21-22
pages
3778 - 3787
publisher
John Wiley & Sons
external identifiers
  • wos:000225679000028
  • pmid:15565687
  • scopus:10944231566
ISSN
0173-0835
DOI
10.1002/elps.200406100
language
English
LU publication?
yes
id
3992643e-9f0b-4e24-a8cb-fb2a7b83efeb (old id 259318)
date added to LUP
2007-11-05 08:05:46
date last changed
2017-01-01 04:43:16
@article{3992643e-9f0b-4e24-a8cb-fb2a7b83efeb,
  abstract     = {A capillary force filling microsystem consisting of a chip-integrated solid-phase microextraction (SMEC) array and a microdispenser for sample purification and trace enrichment of peptides is described. The microextraction array was loaded with solid-phase media (50 mum Poros R2 beads) for purification and enrichment of proteomic samples. Samples bound to the SMEC were eluted in a volume of 200 nL. A piezoelectric microdispenser was docked to the array and the samples bound to the SMEC were eluted in a volume of 200 nL using capillary forces. The purified and enriched samples were dispensed onto the matrix-assisted laser desorption/ionization (MALDI) target, providing quality data from samples in the picomolar range. The nanoproteomic platform was compared to corresponding commercial preparation protocols, showing higher mass spectrometry (MS) signal intensities for peptides generated from an alpha-casein digest. The platform was also elvaluated with regards to two-dimensional (2-D) gel-derived protein digests from both fibroblast and epithelial target cells.},
  author       = {Wallman, Lars and Ekström, Simon and Marko-Varga, György and Laurell, Thomas and Nilsson, Johan},
  issn         = {0173-0835},
  keyword      = {microextraction,miniaturization,mass spectrometry,solid-phase,proteomics},
  language     = {eng},
  number       = {21-22},
  pages        = {3778--3787},
  publisher    = {John Wiley & Sons},
  series       = {Electrophoresis},
  title        = {Autonomous protein sample processing on-chip using solid-phase microextraction, capillary force pumping, and microdispensing},
  url          = {http://dx.doi.org/10.1002/elps.200406100},
  volume       = {25},
  year         = {2004},
}