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Young women with genital prolapse have a low collagen concentration

Westergren Soderberg, M; Falconer, C; Bystrom, B; Malmström, Anders LU and Ekman, G (2004) In Acta Obstetricia et Gynecologica Scandinavica 83(12). p.1193-1198
Abstract
Background. Genital prolapse is a common and handicapping form of pelvic floor dysfunction. To explain its genesis as a result of endopelvic connective tissue weakness, the collagen state was analyzed in women with and without genital prolapse. Methods. Punch biopsies from the paraurethral ligaments were obtained during the operation from 22 women undergoing surgery for genital prolapse. As controls, similar biopsies were taken from 13 women who underwent gynecologic surgery for other benign reasons. Collagen concentration as hydroxyproline and its extractability by pepsin digestion were studied in relation to age by multiple regression, two-way ANOVA, Levene's test, and Student's t-test. Histological examination was also performed.... (More)
Background. Genital prolapse is a common and handicapping form of pelvic floor dysfunction. To explain its genesis as a result of endopelvic connective tissue weakness, the collagen state was analyzed in women with and without genital prolapse. Methods. Punch biopsies from the paraurethral ligaments were obtained during the operation from 22 women undergoing surgery for genital prolapse. As controls, similar biopsies were taken from 13 women who underwent gynecologic surgery for other benign reasons. Collagen concentration as hydroxyproline and its extractability by pepsin digestion were studied in relation to age by multiple regression, two-way ANOVA, Levene's test, and Student's t-test. Histological examination was also performed. Results. Women, younger than 53 years, with genital prolapse had a 30% lower collagen concentration than age-matched controls, which reached significance, P = 0.01. The extractability by pepsin digestion, an indicator of cross-links in the collagen molecule, did not significantly differ between groups. It did, however, decrease significantly with age in both prolapse patient and control groups. Morphology supported these findings with a less-dense extracellular matrix composition subepithelially in genital prolapse compared to a healthy control. Conclusion. For the first time, we show that young women with genital prolapse have a decreased collagen concentration, suggesting a different organization of the endopelvic connective tissue extracellular matrix. Furthermore, these alterations differ from those earlier found in younger women with stress urinary incontinence. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
connective tissue, age, collagen, genital prolapse
in
Acta Obstetricia et Gynecologica Scandinavica
volume
83
issue
12
pages
1193 - 1198
publisher
Wiley-Blackwell
external identifiers
  • wos:000225576200018
  • pmid:15548155
  • scopus:10344249909
ISSN
1600-0412
DOI
10.1111/j.0001-6349.2004.00438.x
language
English
LU publication?
yes
id
a6a98a35-8210-402d-b03d-4e5a345b2406 (old id 259792)
date added to LUP
2007-11-02 08:09:44
date last changed
2017-09-10 04:23:13
@article{a6a98a35-8210-402d-b03d-4e5a345b2406,
  abstract     = {Background. Genital prolapse is a common and handicapping form of pelvic floor dysfunction. To explain its genesis as a result of endopelvic connective tissue weakness, the collagen state was analyzed in women with and without genital prolapse. Methods. Punch biopsies from the paraurethral ligaments were obtained during the operation from 22 women undergoing surgery for genital prolapse. As controls, similar biopsies were taken from 13 women who underwent gynecologic surgery for other benign reasons. Collagen concentration as hydroxyproline and its extractability by pepsin digestion were studied in relation to age by multiple regression, two-way ANOVA, Levene's test, and Student's t-test. Histological examination was also performed. Results. Women, younger than 53 years, with genital prolapse had a 30% lower collagen concentration than age-matched controls, which reached significance, P = 0.01. The extractability by pepsin digestion, an indicator of cross-links in the collagen molecule, did not significantly differ between groups. It did, however, decrease significantly with age in both prolapse patient and control groups. Morphology supported these findings with a less-dense extracellular matrix composition subepithelially in genital prolapse compared to a healthy control. Conclusion. For the first time, we show that young women with genital prolapse have a decreased collagen concentration, suggesting a different organization of the endopelvic connective tissue extracellular matrix. Furthermore, these alterations differ from those earlier found in younger women with stress urinary incontinence.},
  author       = {Westergren Soderberg, M and Falconer, C and Bystrom, B and Malmström, Anders and Ekman, G},
  issn         = {1600-0412},
  keyword      = {connective tissue,age,collagen,genital prolapse},
  language     = {eng},
  number       = {12},
  pages        = {1193--1198},
  publisher    = {Wiley-Blackwell},
  series       = {Acta Obstetricia et Gynecologica Scandinavica},
  title        = {Young women with genital prolapse have a low collagen concentration},
  url          = {http://dx.doi.org/10.1111/j.0001-6349.2004.00438.x},
  volume       = {83},
  year         = {2004},
}