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The β-Ketoacyl-CoA Synthase Hv KCS1, Encoded by Cer-zh, Plays a Key Role in Synthesis of Barley Leaf Wax and Germination of Barley Powdery Mildew

Li, Chao ; Haslam, Tegan M. ; Krger, Anna ; Schneider, Lizette M. LU ; Mishina, Kohei ; Samuels, Lacey ; Yang, Hongxing ; Kunst, Ljerka ; Schaffrath, Ulrich and Nawrath, Christiane , et al. (2018) In Plant and Cell Physiology 59(4). p.806-822
Abstract

The cuticle coats the primary aerial surfaces of land plants. It consists of cutin and waxes, which provide protection against desiccation, pathogens and herbivores. Acyl cuticular waxes are synthesized via elongase complexes that extend fatty acyl precursors up to 38 carbons for downstream modification pathways. The leaves of 21 barley eceriferum (cer) mutants appear to have less or no epicuticular wax crystals, making these mutants excellent tools for identifying elongase and modification pathway biosynthetic genes. Positional cloning of the gene mutated in cer-zh identified an elongase component, β-ketoacyl-CoA synthase (CER-ZH/HvKCS1) that is one of 34 homologous KCSs encoded by the barley genome. The biochemical function of CER-ZH... (More)

The cuticle coats the primary aerial surfaces of land plants. It consists of cutin and waxes, which provide protection against desiccation, pathogens and herbivores. Acyl cuticular waxes are synthesized via elongase complexes that extend fatty acyl precursors up to 38 carbons for downstream modification pathways. The leaves of 21 barley eceriferum (cer) mutants appear to have less or no epicuticular wax crystals, making these mutants excellent tools for identifying elongase and modification pathway biosynthetic genes. Positional cloning of the gene mutated in cer-zh identified an elongase component, β-ketoacyl-CoA synthase (CER-ZH/HvKCS1) that is one of 34 homologous KCSs encoded by the barley genome. The biochemical function of CER-ZH was deduced from wax and cutin analyses and by heterologous expression in yeast. Combined, these experiments revealed that CER-ZH/HvKCS1 has a substrate specificity for C 16 -C 20, especially unsaturated, acyl chains, thus playing a major role in total acyl chain elongation for wax biosynthesis. The contribution of CER-ZH to water barrier properties of the cuticle and its influence on the germination of barley powdery mildew fungus were also assessed.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Blumeria graminis f. sp. Hordei, Cuticular wax, Hordeum vulgare, Very-long-chain fatty acids, β-Ketoacyl-CoA synthase
in
Plant and Cell Physiology
volume
59
issue
4
pages
17 pages
publisher
Oxford University Press
external identifiers
  • pmid:29401261
  • scopus:85045513060
ISSN
0032-0781
DOI
10.1093/pcp/pcy020
language
English
LU publication?
yes
id
25e749ae-031c-43cf-ad85-af94acdc64b9
date added to LUP
2018-04-27 13:52:37
date last changed
2024-07-23 16:48:01
@article{25e749ae-031c-43cf-ad85-af94acdc64b9,
  abstract     = {{<p>The cuticle coats the primary aerial surfaces of land plants. It consists of cutin and waxes, which provide protection against desiccation, pathogens and herbivores. Acyl cuticular waxes are synthesized via elongase complexes that extend fatty acyl precursors up to 38 carbons for downstream modification pathways. The leaves of 21 barley eceriferum (cer) mutants appear to have less or no epicuticular wax crystals, making these mutants excellent tools for identifying elongase and modification pathway biosynthetic genes. Positional cloning of the gene mutated in cer-zh identified an elongase component, β-ketoacyl-CoA synthase (CER-ZH/HvKCS1) that is one of 34 homologous KCSs encoded by the barley genome. The biochemical function of CER-ZH was deduced from wax and cutin analyses and by heterologous expression in yeast. Combined, these experiments revealed that CER-ZH/HvKCS1 has a substrate specificity for C 16 -C 20, especially unsaturated, acyl chains, thus playing a major role in total acyl chain elongation for wax biosynthesis. The contribution of CER-ZH to water barrier properties of the cuticle and its influence on the germination of barley powdery mildew fungus were also assessed.</p>}},
  author       = {{Li, Chao and Haslam, Tegan M. and Krger, Anna and Schneider, Lizette M. and Mishina, Kohei and Samuels, Lacey and Yang, Hongxing and Kunst, Ljerka and Schaffrath, Ulrich and Nawrath, Christiane and Chen, Guoxiong and Komatsuda, Takao and Von Wettstein-Knowles, Penny}},
  issn         = {{0032-0781}},
  keywords     = {{Blumeria graminis f. sp. Hordei; Cuticular wax; Hordeum vulgare; Very-long-chain fatty acids; β-Ketoacyl-CoA synthase}},
  language     = {{eng}},
  month        = {{04}},
  number       = {{4}},
  pages        = {{806--822}},
  publisher    = {{Oxford University Press}},
  series       = {{Plant and Cell Physiology}},
  title        = {{The β-Ketoacyl-CoA Synthase Hv KCS1, Encoded by Cer-zh, Plays a Key Role in Synthesis of Barley Leaf Wax and Germination of Barley Powdery Mildew}},
  url          = {{http://dx.doi.org/10.1093/pcp/pcy020}},
  doi          = {{10.1093/pcp/pcy020}},
  volume       = {{59}},
  year         = {{2018}},
}