The β-Ketoacyl-CoA Synthase Hv KCS1, Encoded by Cer-zh, Plays a Key Role in Synthesis of Barley Leaf Wax and Germination of Barley Powdery Mildew
(2018) In Plant and Cell Physiology 59(4). p.806-822- Abstract
The cuticle coats the primary aerial surfaces of land plants. It consists of cutin and waxes, which provide protection against desiccation, pathogens and herbivores. Acyl cuticular waxes are synthesized via elongase complexes that extend fatty acyl precursors up to 38 carbons for downstream modification pathways. The leaves of 21 barley eceriferum (cer) mutants appear to have less or no epicuticular wax crystals, making these mutants excellent tools for identifying elongase and modification pathway biosynthetic genes. Positional cloning of the gene mutated in cer-zh identified an elongase component, β-ketoacyl-CoA synthase (CER-ZH/HvKCS1) that is one of 34 homologous KCSs encoded by the barley genome. The biochemical function of CER-ZH... (More)
The cuticle coats the primary aerial surfaces of land plants. It consists of cutin and waxes, which provide protection against desiccation, pathogens and herbivores. Acyl cuticular waxes are synthesized via elongase complexes that extend fatty acyl precursors up to 38 carbons for downstream modification pathways. The leaves of 21 barley eceriferum (cer) mutants appear to have less or no epicuticular wax crystals, making these mutants excellent tools for identifying elongase and modification pathway biosynthetic genes. Positional cloning of the gene mutated in cer-zh identified an elongase component, β-ketoacyl-CoA synthase (CER-ZH/HvKCS1) that is one of 34 homologous KCSs encoded by the barley genome. The biochemical function of CER-ZH was deduced from wax and cutin analyses and by heterologous expression in yeast. Combined, these experiments revealed that CER-ZH/HvKCS1 has a substrate specificity for C 16 -C 20, especially unsaturated, acyl chains, thus playing a major role in total acyl chain elongation for wax biosynthesis. The contribution of CER-ZH to water barrier properties of the cuticle and its influence on the germination of barley powdery mildew fungus were also assessed.
(Less)
- author
- organization
- publishing date
- 2018-04-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Blumeria graminis f. sp. Hordei, Cuticular wax, Hordeum vulgare, Very-long-chain fatty acids, β-Ketoacyl-CoA synthase
- in
- Plant and Cell Physiology
- volume
- 59
- issue
- 4
- pages
- 17 pages
- publisher
- Oxford University Press
- external identifiers
-
- pmid:29401261
- scopus:85045513060
- ISSN
- 0032-0781
- DOI
- 10.1093/pcp/pcy020
- language
- English
- LU publication?
- yes
- id
- 25e749ae-031c-43cf-ad85-af94acdc64b9
- date added to LUP
- 2018-04-27 13:52:37
- date last changed
- 2024-07-23 16:48:01
@article{25e749ae-031c-43cf-ad85-af94acdc64b9, abstract = {{<p>The cuticle coats the primary aerial surfaces of land plants. It consists of cutin and waxes, which provide protection against desiccation, pathogens and herbivores. Acyl cuticular waxes are synthesized via elongase complexes that extend fatty acyl precursors up to 38 carbons for downstream modification pathways. The leaves of 21 barley eceriferum (cer) mutants appear to have less or no epicuticular wax crystals, making these mutants excellent tools for identifying elongase and modification pathway biosynthetic genes. Positional cloning of the gene mutated in cer-zh identified an elongase component, β-ketoacyl-CoA synthase (CER-ZH/HvKCS1) that is one of 34 homologous KCSs encoded by the barley genome. The biochemical function of CER-ZH was deduced from wax and cutin analyses and by heterologous expression in yeast. Combined, these experiments revealed that CER-ZH/HvKCS1 has a substrate specificity for C 16 -C 20, especially unsaturated, acyl chains, thus playing a major role in total acyl chain elongation for wax biosynthesis. The contribution of CER-ZH to water barrier properties of the cuticle and its influence on the germination of barley powdery mildew fungus were also assessed.</p>}}, author = {{Li, Chao and Haslam, Tegan M. and Krger, Anna and Schneider, Lizette M. and Mishina, Kohei and Samuels, Lacey and Yang, Hongxing and Kunst, Ljerka and Schaffrath, Ulrich and Nawrath, Christiane and Chen, Guoxiong and Komatsuda, Takao and Von Wettstein-Knowles, Penny}}, issn = {{0032-0781}}, keywords = {{Blumeria graminis f. sp. Hordei; Cuticular wax; Hordeum vulgare; Very-long-chain fatty acids; β-Ketoacyl-CoA synthase}}, language = {{eng}}, month = {{04}}, number = {{4}}, pages = {{806--822}}, publisher = {{Oxford University Press}}, series = {{Plant and Cell Physiology}}, title = {{The β-Ketoacyl-CoA Synthase Hv KCS1, Encoded by Cer-zh, Plays a Key Role in Synthesis of Barley Leaf Wax and Germination of Barley Powdery Mildew}}, url = {{http://dx.doi.org/10.1093/pcp/pcy020}}, doi = {{10.1093/pcp/pcy020}}, volume = {{59}}, year = {{2018}}, }