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RNAi screen identifies MAPK14 as a druggable suppressor of human hematopoietic stem cell expansion.

Baudet, Aurelie LU ; Karlsson, Christine LU ; Safaee Talkhoncheh, Mehrnaz LU ; Galeev, Roman LU ; Magnusson, Mattias LU and Larsson, Jonas LU (2012) In Blood 119(26). p.6255-6258
Abstract
We report on a forward RNAi screen in primary human hematopoietic stem and progenitor cells, using pooled lentiviral shRNA libraries deconvoluted by next generation sequencing. We identify MAPK14/p38α as a modulator of ex vivo stem cell proliferation and show that pharmacological inhibition of p38 dramatically enhances the stem cell activity of cultured umbilical cord blood derived hematopoietic cells. p38 inhibitors should thus be considered in strategies aiming at expanding stem cells for clinical benefit.
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Blood
volume
119
issue
26
pages
6255 - 6258
publisher
American Society of Hematology
external identifiers
  • wos:000307400700016
  • pmid:22555972
  • scopus:84863520113
ISSN
1528-0020
DOI
10.1182/blood-2012-01-403949
language
English
LU publication?
yes
id
9617e44f-9812-407c-a5df-bb3fd8241d2b (old id 2609154)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/22555972?dopt=Abstract
date added to LUP
2012-06-02 16:08:15
date last changed
2017-10-22 04:54:05
@article{9617e44f-9812-407c-a5df-bb3fd8241d2b,
  abstract     = {We report on a forward RNAi screen in primary human hematopoietic stem and progenitor cells, using pooled lentiviral shRNA libraries deconvoluted by next generation sequencing. We identify MAPK14/p38α as a modulator of ex vivo stem cell proliferation and show that pharmacological inhibition of p38 dramatically enhances the stem cell activity of cultured umbilical cord blood derived hematopoietic cells. p38 inhibitors should thus be considered in strategies aiming at expanding stem cells for clinical benefit.},
  author       = {Baudet, Aurelie and Karlsson, Christine and Safaee Talkhoncheh, Mehrnaz and Galeev, Roman and Magnusson, Mattias and Larsson, Jonas},
  issn         = {1528-0020},
  language     = {eng},
  number       = {26},
  pages        = {6255--6258},
  publisher    = {American Society of Hematology},
  series       = {Blood},
  title        = {RNAi screen identifies MAPK14 as a druggable suppressor of human hematopoietic stem cell expansion.},
  url          = {http://dx.doi.org/10.1182/blood-2012-01-403949},
  volume       = {119},
  year         = {2012},
}