RNAi screen identifies MAPK14 as a druggable suppressor of human hematopoietic stem cell expansion.
(2012) In Blood 119(26). p.6255-6258- Abstract
- We report on a forward RNAi screen in primary human hematopoietic stem and progenitor cells, using pooled lentiviral shRNA libraries deconvoluted by next generation sequencing. We identify MAPK14/p38α as a modulator of ex vivo stem cell proliferation and show that pharmacological inhibition of p38 dramatically enhances the stem cell activity of cultured umbilical cord blood derived hematopoietic cells. p38 inhibitors should thus be considered in strategies aiming at expanding stem cells for clinical benefit.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2609154
- author
- Baudet, Aurelie LU ; Karlsson, Christine LU ; Safaee Talkhoncheh, Mehrnaz LU ; Galeev, Roman LU ; Magnusson, Mattias LU and Larsson, Jonas LU
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Blood
- volume
- 119
- issue
- 26
- pages
- 6255 - 6258
- publisher
- American Society of Hematology
- external identifiers
-
- wos:000307400700016
- pmid:22555972
- scopus:84863520113
- pmid:22555972
- ISSN
- 1528-0020
- DOI
- 10.1182/blood-2012-01-403949
- language
- English
- LU publication?
- yes
- id
- 9617e44f-9812-407c-a5df-bb3fd8241d2b (old id 2609154)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/22555972?dopt=Abstract
- date added to LUP
- 2016-04-04 08:21:52
- date last changed
- 2024-01-27 01:57:26
@article{9617e44f-9812-407c-a5df-bb3fd8241d2b, abstract = {{We report on a forward RNAi screen in primary human hematopoietic stem and progenitor cells, using pooled lentiviral shRNA libraries deconvoluted by next generation sequencing. We identify MAPK14/p38α as a modulator of ex vivo stem cell proliferation and show that pharmacological inhibition of p38 dramatically enhances the stem cell activity of cultured umbilical cord blood derived hematopoietic cells. p38 inhibitors should thus be considered in strategies aiming at expanding stem cells for clinical benefit.}}, author = {{Baudet, Aurelie and Karlsson, Christine and Safaee Talkhoncheh, Mehrnaz and Galeev, Roman and Magnusson, Mattias and Larsson, Jonas}}, issn = {{1528-0020}}, language = {{eng}}, number = {{26}}, pages = {{6255--6258}}, publisher = {{American Society of Hematology}}, series = {{Blood}}, title = {{RNAi screen identifies MAPK14 as a druggable suppressor of human hematopoietic stem cell expansion.}}, url = {{http://dx.doi.org/10.1182/blood-2012-01-403949}}, doi = {{10.1182/blood-2012-01-403949}}, volume = {{119}}, year = {{2012}}, }