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Methods and Models for Metabolic Assessment in Mice

Pacini, G. ; Omar, Bilal LU and Ahrén, Bo LU (2013) In Journal of Diabetes Research
Abstract
The development of new therapies for the treatment of type 2 diabetes requires robust, reproducible and well validated in vivo experimental systems. Mice provide the most ideal animal model for studies of potential therapies. Unlike larger animals, mice have a short gestational period, are genetically similar, often give birth to many offspring at once and can be housed as multiple groups in a single cage. The mouse model has been extensively metabolically characterized using different tests. This report summarizes how these tests can be executed and how arising data are analyzed to confidently determine changes in insulin resistance and insulin secretion with high reproducibility. The main tests for metabolic assessment in the mouse... (More)
The development of new therapies for the treatment of type 2 diabetes requires robust, reproducible and well validated in vivo experimental systems. Mice provide the most ideal animal model for studies of potential therapies. Unlike larger animals, mice have a short gestational period, are genetically similar, often give birth to many offspring at once and can be housed as multiple groups in a single cage. The mouse model has been extensively metabolically characterized using different tests. This report summarizes how these tests can be executed and how arising data are analyzed to confidently determine changes in insulin resistance and insulin secretion with high reproducibility. The main tests for metabolic assessment in the mouse reviewed here are the glucose clamp, the intravenous and the oral glucose tolerance tests. For all these experiments, including some commonly adopted variants, we describe: (i) their performance; (ii) their advantages and limitations; (iii) the empirical formulas and mathematical models implemented for the analysis of the data arising from the experimental procedures to obtain reliable measurements of peripheral insulin sensitivity and beta cell function. Finally, a list of previous applications of these methods and analytical techniques is provided to better comprehend their use and the evidences that these studies yielded. (Less)
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author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Diabetes Research
article number
986906
publisher
Hindawi Limited
external identifiers
  • wos:000319926700001
  • scopus:84878695632
  • pmid:23762879
ISSN
2314-6753
DOI
10.1155/2013/986906
language
English
LU publication?
yes
id
26105b9b-273e-4987-87fb-99f4802ddee2 (old id 3930452)
date added to LUP
2016-04-01 10:09:47
date last changed
2024-01-21 06:59:14
@article{26105b9b-273e-4987-87fb-99f4802ddee2,
  abstract     = {{The development of new therapies for the treatment of type 2 diabetes requires robust, reproducible and well validated in vivo experimental systems. Mice provide the most ideal animal model for studies of potential therapies. Unlike larger animals, mice have a short gestational period, are genetically similar, often give birth to many offspring at once and can be housed as multiple groups in a single cage. The mouse model has been extensively metabolically characterized using different tests. This report summarizes how these tests can be executed and how arising data are analyzed to confidently determine changes in insulin resistance and insulin secretion with high reproducibility. The main tests for metabolic assessment in the mouse reviewed here are the glucose clamp, the intravenous and the oral glucose tolerance tests. For all these experiments, including some commonly adopted variants, we describe: (i) their performance; (ii) their advantages and limitations; (iii) the empirical formulas and mathematical models implemented for the analysis of the data arising from the experimental procedures to obtain reliable measurements of peripheral insulin sensitivity and beta cell function. Finally, a list of previous applications of these methods and analytical techniques is provided to better comprehend their use and the evidences that these studies yielded.}},
  author       = {{Pacini, G. and Omar, Bilal and Ahrén, Bo}},
  issn         = {{2314-6753}},
  language     = {{eng}},
  publisher    = {{Hindawi Limited}},
  series       = {{Journal of Diabetes Research}},
  title        = {{Methods and Models for Metabolic Assessment in Mice}},
  url          = {{https://lup.lub.lu.se/search/files/1616565/4145670.pdf}},
  doi          = {{10.1155/2013/986906}},
  year         = {{2013}},
}