Extraction of natural moisturizing factor from the stratum corneum and its implication on skin molecular mobility
(2021) In Journal of Colloid and Interface Science 604. p.480-491- Abstract
The natural moisturizing factor (NMF) is a mixture of small water-soluble compounds present in the upper layer of the skin, stratum corneum (SC). Soaking of SC in water leads to extraction of the NMF molecules, which may influence the SC molecular properties and lead to brittle and dry skin. In this study, we investigate how the molecular dynamics in SC lipid and protein components are affected by the removal of the NMF compounds. We then explore whether the changes in SC components caused by NMF removal can be reversed by a subsequent addition of one single NMF component: urea, pyrrolidone carboxylic acid (PCA) or potassium lactate. Samples of intact SC were investigated using NMR, X-ray diffraction, infrared spectroscopy and sorption... (More)
The natural moisturizing factor (NMF) is a mixture of small water-soluble compounds present in the upper layer of the skin, stratum corneum (SC). Soaking of SC in water leads to extraction of the NMF molecules, which may influence the SC molecular properties and lead to brittle and dry skin. In this study, we investigate how the molecular dynamics in SC lipid and protein components are affected by the removal of the NMF compounds. We then explore whether the changes in SC components caused by NMF removal can be reversed by a subsequent addition of one single NMF component: urea, pyrrolidone carboxylic acid (PCA) or potassium lactate. Samples of intact SC were investigated using NMR, X-ray diffraction, infrared spectroscopy and sorption microbalance. It is shown that the removal of NMF leads to reduced molecular mobility in keratin filaments and SC lipids compared to untreated SC. When the complex NMF mixture is replaced by one single NMF component, the molecular mobility in both keratin filaments and lipids is regained. From this we propose a general relation between the molecular mobility in SC and the amount of polar solutes which does not appear specific to the precise chemical identify of the NMF compounds.
(Less)
- author
- Gunnarsson, Maria LU ; Mojumdar, Enamul Haque LU ; Topgaard, Daniel LU and Sparr, Emma LU
- organization
- publishing date
- 2021-12
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- ATR-FTIR, Natural moisturizing factor, NMF, SAXD, Solid-state NMR, Stratum corneum, WAXD
- in
- Journal of Colloid and Interface Science
- volume
- 604
- pages
- 12 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:34273783
- scopus:85110022240
- ISSN
- 0021-9797
- DOI
- 10.1016/j.jcis.2021.07.012
- language
- English
- LU publication?
- yes
- id
- 2611dd94-bc35-4588-b993-f9798aa26510
- date added to LUP
- 2021-12-23 11:01:08
- date last changed
- 2024-04-20 19:18:06
@article{2611dd94-bc35-4588-b993-f9798aa26510, abstract = {{<p>The natural moisturizing factor (NMF) is a mixture of small water-soluble compounds present in the upper layer of the skin, stratum corneum (SC). Soaking of SC in water leads to extraction of the NMF molecules, which may influence the SC molecular properties and lead to brittle and dry skin. In this study, we investigate how the molecular dynamics in SC lipid and protein components are affected by the removal of the NMF compounds. We then explore whether the changes in SC components caused by NMF removal can be reversed by a subsequent addition of one single NMF component: urea, pyrrolidone carboxylic acid (PCA) or potassium lactate. Samples of intact SC were investigated using NMR, X-ray diffraction, infrared spectroscopy and sorption microbalance. It is shown that the removal of NMF leads to reduced molecular mobility in keratin filaments and SC lipids compared to untreated SC. When the complex NMF mixture is replaced by one single NMF component, the molecular mobility in both keratin filaments and lipids is regained. From this we propose a general relation between the molecular mobility in SC and the amount of polar solutes which does not appear specific to the precise chemical identify of the NMF compounds.</p>}}, author = {{Gunnarsson, Maria and Mojumdar, Enamul Haque and Topgaard, Daniel and Sparr, Emma}}, issn = {{0021-9797}}, keywords = {{ATR-FTIR; Natural moisturizing factor; NMF; SAXD; Solid-state NMR; Stratum corneum; WAXD}}, language = {{eng}}, pages = {{480--491}}, publisher = {{Elsevier}}, series = {{Journal of Colloid and Interface Science}}, title = {{Extraction of natural moisturizing factor from the stratum corneum and its implication on skin molecular mobility}}, url = {{http://dx.doi.org/10.1016/j.jcis.2021.07.012}}, doi = {{10.1016/j.jcis.2021.07.012}}, volume = {{604}}, year = {{2021}}, }