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Leukocyte adhesion in aorta and femoral artery in vivo is mediated by LFA-1

Schramm, R; Menger, M D; Schaefers, H J and Thorlacius, Henrik LU (2004) In Inflammation Research1995-01-01+01:00 53(10). p.523-527
Abstract
Objective: Cytokine-induced recruitment of leukocytes is an early feature during arterial injury and atherosclerotic plaque formation. The aim of this study was to analyze the role of the beta(2)-integrin lymphocyte function-associated antigen-1 (LFA- 1; CD11a/CD18) in cytokine-triggered firm leukocyte adhesion to arterial endothelium in vivo. Material and Methods: Intravital fluorescence microscopy was used to study leukocyte firm adhesion in the mouse aorta and femoral artery in response to combined local challenge with TNF-alpha and IL-1beta. Results: In wild-type (WT) mice, cytokine stimulation resulted in firm adhesion of 14.6 +/- 2.8 and 11.3 +/- 1.3 leukocytes/mm along the endothelium in the aorta and femoral artery (P < 0.05 vs.... (More)
Objective: Cytokine-induced recruitment of leukocytes is an early feature during arterial injury and atherosclerotic plaque formation. The aim of this study was to analyze the role of the beta(2)-integrin lymphocyte function-associated antigen-1 (LFA- 1; CD11a/CD18) in cytokine-triggered firm leukocyte adhesion to arterial endothelium in vivo. Material and Methods: Intravital fluorescence microscopy was used to study leukocyte firm adhesion in the mouse aorta and femoral artery in response to combined local challenge with TNF-alpha and IL-1beta. Results: In wild-type (WT) mice, cytokine stimulation resulted in firm adhesion of 14.6 +/- 2.8 and 11.3 +/- 1.3 leukocytes/mm along the endothelium in the aorta and femoral artery (P < 0.05 vs. PBS-treated controls, n = 5-6). Notably, the number of firmly adherent leukocytes in aorta and femoral artery of cytokine-stimulated LFA-1-deficient animals was reduced by 54% and 92% indicating an important role of LFA-1 in leukocyte adhesion to arterial endothelium (P < 0.05 vs. controls, n = 5-6). In addition, pretreatment of WT mice with a monoclonal antibody (mAb) directed against murine LFA-1 attenuated the leukocyte adhesive response by 60% and 86% in aorta and femoral artery, respectively (P < 0.05 vs. control mAb-treated WT, n = 5-12). Conclusion: These novel data demonstrate that cytokine-induced firm leukocyte adhesion in the mouse aorta and femoral artery is LFA-1-dependent in vivo, which may implicate an important role for this β(2)-integrin leukocyte extravasation. in arterial injury and atherogenesis. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
leukocyte recruitment, beta(2)-integrins, inflammation, atherosclerosis, cytokines
in
Inflammation Research1995-01-01+01:00
volume
53
issue
10
pages
523 - 527
publisher
Birkhaüser
external identifiers
  • wos:000224915700004
  • pmid:15597146
  • scopus:12144262055
ISSN
1420-908X
DOI
10.1007/s00011-004-1285-x
language
English
LU publication?
yes
id
7327e833-b02a-4284-8b24-5b38e822d822 (old id 262442)
date added to LUP
2007-10-26 13:43:53
date last changed
2017-01-01 05:18:13
@article{7327e833-b02a-4284-8b24-5b38e822d822,
  abstract     = {Objective: Cytokine-induced recruitment of leukocytes is an early feature during arterial injury and atherosclerotic plaque formation. The aim of this study was to analyze the role of the beta(2)-integrin lymphocyte function-associated antigen-1 (LFA- 1; CD11a/CD18) in cytokine-triggered firm leukocyte adhesion to arterial endothelium in vivo. Material and Methods: Intravital fluorescence microscopy was used to study leukocyte firm adhesion in the mouse aorta and femoral artery in response to combined local challenge with TNF-alpha and IL-1beta. Results: In wild-type (WT) mice, cytokine stimulation resulted in firm adhesion of 14.6 +/- 2.8 and 11.3 +/- 1.3 leukocytes/mm along the endothelium in the aorta and femoral artery (P &lt; 0.05 vs. PBS-treated controls, n = 5-6). Notably, the number of firmly adherent leukocytes in aorta and femoral artery of cytokine-stimulated LFA-1-deficient animals was reduced by 54% and 92% indicating an important role of LFA-1 in leukocyte adhesion to arterial endothelium (P &lt; 0.05 vs. controls, n = 5-6). In addition, pretreatment of WT mice with a monoclonal antibody (mAb) directed against murine LFA-1 attenuated the leukocyte adhesive response by 60% and 86% in aorta and femoral artery, respectively (P &lt; 0.05 vs. control mAb-treated WT, n = 5-12). Conclusion: These novel data demonstrate that cytokine-induced firm leukocyte adhesion in the mouse aorta and femoral artery is LFA-1-dependent in vivo, which may implicate an important role for this β(2)-integrin leukocyte extravasation. in arterial injury and atherogenesis.},
  author       = {Schramm, R and Menger, M D and Schaefers, H J and Thorlacius, Henrik},
  issn         = {1420-908X},
  keyword      = {leukocyte recruitment,beta(2)-integrins,inflammation,atherosclerosis,cytokines},
  language     = {eng},
  number       = {10},
  pages        = {523--527},
  publisher    = {Birkhaüser},
  series       = {Inflammation Research1995-01-01+01:00},
  title        = {Leukocyte adhesion in aorta and femoral artery in vivo is mediated by LFA-1},
  url          = {http://dx.doi.org/10.1007/s00011-004-1285-x},
  volume       = {53},
  year         = {2004},
}