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The intracellular helical bundle of human glucose transporter GLUT4 is important for complex formation with ASPL

Huang, Peng LU ; Åbacka, Hannah LU orcid ; Varela, Daniel LU orcid ; Venskutonytė, Raminta LU ; Happonen, Lotta LU ; Bogan, Jonathan S. ; Gourdon, Pontus LU ; Amiry-Moghaddam, Mahmood R. ; André, Ingmar and Lindkvist-Petersson, Karin LU (2023) In FEBS Open Bio 13(11). p.2094-2107
Abstract

Glucose transporters (GLUTs) are responsible for transporting hexose molecules across cellular membranes. In adipocytes, insulin stimulates glucose uptake by redistributing GLUT4 to the plasma membrane. In unstimulated adipose-like mouse cell lines, GLUT4 is known to be retained intracellularly by binding to TUG protein, while upon insulin stimulation, GLUT4 dissociates from TUG. Here, we report that the TUG homolog in human, ASPL, exerts similar properties, i.e., forms a complex with GLUT4. We describe the structural details of complex formation by combining biochemical assays with cross-linking mass spectrometry and computational modeling. Combined, the data suggest that the intracellular domain of GLUT4 binds to the helical lariat of... (More)

Glucose transporters (GLUTs) are responsible for transporting hexose molecules across cellular membranes. In adipocytes, insulin stimulates glucose uptake by redistributing GLUT4 to the plasma membrane. In unstimulated adipose-like mouse cell lines, GLUT4 is known to be retained intracellularly by binding to TUG protein, while upon insulin stimulation, GLUT4 dissociates from TUG. Here, we report that the TUG homolog in human, ASPL, exerts similar properties, i.e., forms a complex with GLUT4. We describe the structural details of complex formation by combining biochemical assays with cross-linking mass spectrometry and computational modeling. Combined, the data suggest that the intracellular domain of GLUT4 binds to the helical lariat of ASPL and contributes to the regulation of GLUT4 trafficking by cooperative binding.

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author
; ; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
adipocyte, ASPL, glucose transporters, GLUT4, trafficking, TUG
in
FEBS Open Bio
volume
13
issue
11
pages
2094 - 2107
publisher
Wiley-Blackwell
external identifiers
  • pmid:37731227
  • scopus:85173974503
ISSN
2211-5463
DOI
10.1002/2211-5463.13709
language
English
LU publication?
yes
additional info
Publisher Copyright: © 2023 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.
id
262c2831-91d8-4d97-9f92-55d6e268bd85
date added to LUP
2023-10-31 05:52:32
date last changed
2024-04-19 03:05:55
@article{262c2831-91d8-4d97-9f92-55d6e268bd85,
  abstract     = {{<p>Glucose transporters (GLUTs) are responsible for transporting hexose molecules across cellular membranes. In adipocytes, insulin stimulates glucose uptake by redistributing GLUT4 to the plasma membrane. In unstimulated adipose-like mouse cell lines, GLUT4 is known to be retained intracellularly by binding to TUG protein, while upon insulin stimulation, GLUT4 dissociates from TUG. Here, we report that the TUG homolog in human, ASPL, exerts similar properties, i.e., forms a complex with GLUT4. We describe the structural details of complex formation by combining biochemical assays with cross-linking mass spectrometry and computational modeling. Combined, the data suggest that the intracellular domain of GLUT4 binds to the helical lariat of ASPL and contributes to the regulation of GLUT4 trafficking by cooperative binding.</p>}},
  author       = {{Huang, Peng and Åbacka, Hannah and Varela, Daniel and Venskutonytė, Raminta and Happonen, Lotta and Bogan, Jonathan S. and Gourdon, Pontus and Amiry-Moghaddam, Mahmood R. and André, Ingmar and Lindkvist-Petersson, Karin}},
  issn         = {{2211-5463}},
  keywords     = {{adipocyte; ASPL; glucose transporters; GLUT4; trafficking; TUG}},
  language     = {{eng}},
  number       = {{11}},
  pages        = {{2094--2107}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{FEBS Open Bio}},
  title        = {{The intracellular helical bundle of human glucose transporter GLUT4 is important for complex formation with ASPL}},
  url          = {{http://dx.doi.org/10.1002/2211-5463.13709}},
  doi          = {{10.1002/2211-5463.13709}},
  volume       = {{13}},
  year         = {{2023}},
}