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High-Flow-Rate Impinger for the Study of Concentration, Viability, Metabolic Activity, and Ice-Nucleation Activity of Airborne Bacteria

Santl-Temkiv, Tina LU ; Amato, Pierre; Gosewinkel Karlson, Ulrich; Thyrhaug, Runar; Charton, Anaïs; Chicot, Benjamin; Finster, Kai; Bratbak, Gunnar and Löndahl, Jakob LU (2017) In Environmental Science and Technology 51(19). p.11224-11234
Abstract

The study of airborne bacteria relies on a sampling strategy that preserves their integrity and in situ physiological state, e.g. viability, cultivability, metabolic activity, and ice-nucleation activity. Because ambient air harbors low concentrations of bacteria, an effective bioaerosol sampler should have a high sampling efficiency and a high airflow. We characterize a high-flow-rate impinger with respect to particle collection and retention efficiencies in the range 0.5-3.0 μm, and we investigated its ability to preserve the physiological state of selected bacterial species and seawater bacterial community in comparison with four commercial bioaerosol samplers. The collection efficiency increased with particle size and the cutoff... (More)

The study of airborne bacteria relies on a sampling strategy that preserves their integrity and in situ physiological state, e.g. viability, cultivability, metabolic activity, and ice-nucleation activity. Because ambient air harbors low concentrations of bacteria, an effective bioaerosol sampler should have a high sampling efficiency and a high airflow. We characterize a high-flow-rate impinger with respect to particle collection and retention efficiencies in the range 0.5-3.0 μm, and we investigated its ability to preserve the physiological state of selected bacterial species and seawater bacterial community in comparison with four commercial bioaerosol samplers. The collection efficiency increased with particle size and the cutoff diameter was between 0.5 and 1 μm. During sampling periods of 120-300 min, the impinger retained the cultivability, metabolic activity, viability, and ice-nucleation activity of investigated bacteria. Field studies in semiurban, high-altitude, and polar environments included periods of low bacterial air concentrations, thus demonstrating the benefits of the impinger's high flow rate. In conclusion, the impinger described here has many advantages compared with other bioaerosol samplers currently on the market: a potential for long sampling time, a high flow rate, a high sampling and retention efficiency, low costs, and applicability for diverse downstream microbiological and molecular analyses.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Environmental Science and Technology
volume
51
issue
19
pages
11 pages
publisher
The American Chemical Society
external identifiers
  • scopus:85030684317
  • wos:000412716500034
ISSN
0013-936X
DOI
10.1021/acs.est.7b01480
language
English
LU publication?
yes
id
2638e145-c1cd-49ed-ac9b-f2199bb0f322
date added to LUP
2017-10-16 11:50:12
date last changed
2018-05-20 04:38:08
@article{2638e145-c1cd-49ed-ac9b-f2199bb0f322,
  abstract     = {<p>The study of airborne bacteria relies on a sampling strategy that preserves their integrity and in situ physiological state, e.g. viability, cultivability, metabolic activity, and ice-nucleation activity. Because ambient air harbors low concentrations of bacteria, an effective bioaerosol sampler should have a high sampling efficiency and a high airflow. We characterize a high-flow-rate impinger with respect to particle collection and retention efficiencies in the range 0.5-3.0 μm, and we investigated its ability to preserve the physiological state of selected bacterial species and seawater bacterial community in comparison with four commercial bioaerosol samplers. The collection efficiency increased with particle size and the cutoff diameter was between 0.5 and 1 μm. During sampling periods of 120-300 min, the impinger retained the cultivability, metabolic activity, viability, and ice-nucleation activity of investigated bacteria. Field studies in semiurban, high-altitude, and polar environments included periods of low bacterial air concentrations, thus demonstrating the benefits of the impinger's high flow rate. In conclusion, the impinger described here has many advantages compared with other bioaerosol samplers currently on the market: a potential for long sampling time, a high flow rate, a high sampling and retention efficiency, low costs, and applicability for diverse downstream microbiological and molecular analyses.</p>},
  author       = {Santl-Temkiv, Tina and Amato, Pierre and Gosewinkel Karlson, Ulrich and Thyrhaug, Runar and Charton, Anaïs and Chicot, Benjamin and Finster, Kai and Bratbak, Gunnar and Löndahl, Jakob},
  issn         = {0013-936X},
  language     = {eng},
  month        = {10},
  number       = {19},
  pages        = {11224--11234},
  publisher    = {The American Chemical Society},
  series       = {Environmental Science and Technology},
  title        = {High-Flow-Rate Impinger for the Study of Concentration, Viability, Metabolic Activity, and Ice-Nucleation Activity of Airborne Bacteria},
  url          = {http://dx.doi.org/10.1021/acs.est.7b01480},
  volume       = {51},
  year         = {2017},
}