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Exogenous matrix precursors promote functional nerve regeneration across a 15-mm gap within a silicone chamber in the rat

Williams, L R LU ; Danielsen, N LU ; Müller, H and Varon, S (1987) In Journal of Comparative Neurology 264(2). p.90-284
Abstract

When silicone regeneration chambers are implanted empty, axonal regeneration fails if the interstump gap length is greater than 10 mm. Previous experiments using the 10-mm gap model demonstrated that regeneration success correlated with the dimension and/or consistency of the naturally formed acellular fibrin matrix. Both spatial and temporal parameters of regeneration could be stimulated through modifications of the fibrin matrix by prefilling the chambers at the time of implantation either with phosphate-buffered saline or plasma dialyzed against phosphate-buffered saline. In the present experiments, similar modification of matrix formation was found to promote successful regeneration across 15-mm and 20-mm interstump gap lengths. In... (More)

When silicone regeneration chambers are implanted empty, axonal regeneration fails if the interstump gap length is greater than 10 mm. Previous experiments using the 10-mm gap model demonstrated that regeneration success correlated with the dimension and/or consistency of the naturally formed acellular fibrin matrix. Both spatial and temporal parameters of regeneration could be stimulated through modifications of the fibrin matrix by prefilling the chambers at the time of implantation either with phosphate-buffered saline or plasma dialyzed against phosphate-buffered saline. In the present experiments, similar modification of matrix formation was found to promote successful regeneration across 15-mm and 20-mm interstump gap lengths. In addition, prefilling 15-mm-gap chambers with dialyzed plasma resulted in a 3.5-fold increase in the incidence of functional restitution detected at 8 weeks after implantation over the outcome with chambers prefilled with phosphate-buffered saline.

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author
publishing date
type
Contribution to journal
publication status
published
keywords
Animals, Axons, Buffers, Female, Nerve Regeneration, Phosphates, Plasma, Rats, Rats, Inbred Strains, Sciatic Nerve, Sodium Chloride, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.
in
Journal of Comparative Neurology
volume
264
issue
2
pages
90 - 284
publisher
John Wiley & Sons
external identifiers
  • scopus:0023522672
ISSN
0021-9967
DOI
10.1002/cne.902640211
language
English
LU publication?
no
id
2647d91d-240b-42cf-ad37-6383bf7bf508
date added to LUP
2017-10-13 13:46:06
date last changed
2017-11-06 16:06:31
@article{2647d91d-240b-42cf-ad37-6383bf7bf508,
  abstract     = {<p>When silicone regeneration chambers are implanted empty, axonal regeneration fails if the interstump gap length is greater than 10 mm. Previous experiments using the 10-mm gap model demonstrated that regeneration success correlated with the dimension and/or consistency of the naturally formed acellular fibrin matrix. Both spatial and temporal parameters of regeneration could be stimulated through modifications of the fibrin matrix by prefilling the chambers at the time of implantation either with phosphate-buffered saline or plasma dialyzed against phosphate-buffered saline. In the present experiments, similar modification of matrix formation was found to promote successful regeneration across 15-mm and 20-mm interstump gap lengths. In addition, prefilling 15-mm-gap chambers with dialyzed plasma resulted in a 3.5-fold increase in the incidence of functional restitution detected at 8 weeks after implantation over the outcome with chambers prefilled with phosphate-buffered saline.</p>},
  author       = {Williams, L R and Danielsen, N and Müller, H and Varon, S},
  issn         = {0021-9967},
  keyword      = {Animals,Axons,Buffers,Female,Nerve Regeneration,Phosphates,Plasma,Rats,Rats, Inbred Strains,Sciatic Nerve,Sodium Chloride,Journal Article,Research Support, Non-U.S. Gov't,Research Support, U.S. Gov't, Non-P.H.S.},
  language     = {eng},
  month        = {10},
  number       = {2},
  pages        = {90--284},
  publisher    = {John Wiley & Sons},
  series       = {Journal of Comparative Neurology},
  title        = {Exogenous matrix precursors promote functional nerve regeneration across a 15-mm gap within a silicone chamber in the rat},
  url          = {http://dx.doi.org/10.1002/cne.902640211},
  volume       = {264},
  year         = {1987},
}