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Cell-Free Hemoglobin Concentration in Blood Prime Solution Is a Major Determinant of Cell-Free Hemoglobin Exposure during Cardiopulmonary Bypass Circulation in the Newborn

Jungner, Åsa LU ; Vallius, Suvi LU ; Gram, Magnus LU orcid and Ley, David LU (2022) In Journal of Clinical Medicine 11(14).
Abstract

Exposure to circulating cell-free hemoglobin is a ubiquitous feature of open-heart surgery on cardiopulmonary bypass circulation. This study aims to determine the origins and dynamics of circulating cell-free hemoglobin and its major scavenger proteins haptoglobin and hemopexin during neonatal cardiopulmonary bypass. Forty neonates with an isolated critical congenital heart defect were included in a single-center prospective observational study. Blood samples were obtained preoperatively, hourly during bypass circulation, after bypass separation, at admission to the pediatric intensive care unit, and at postoperative days 1–3. Concentrations of cell-free hemoglobin, haptoglobin and hemopexin were determined using ELISA. Neonates were... (More)

Exposure to circulating cell-free hemoglobin is a ubiquitous feature of open-heart surgery on cardiopulmonary bypass circulation. This study aims to determine the origins and dynamics of circulating cell-free hemoglobin and its major scavenger proteins haptoglobin and hemopexin during neonatal cardiopulmonary bypass. Forty neonates with an isolated critical congenital heart defect were included in a single-center prospective observational study. Blood samples were obtained preoperatively, hourly during bypass circulation, after bypass separation, at admission to the pediatric intensive care unit, and at postoperative days 1–3. Concentrations of cell-free hemoglobin, haptoglobin and hemopexin were determined using ELISA. Neonates were exposed to significantly elevated plasma concentrations of cell-free hemoglobin and a concomitant depletion of scavenger protein supplies during open-heart surgery. The main predictor of cell-free hemoglobin exposure was the concentration of cell-free hemoglobin in blood prime solution. Concentrations of haptoglobin and hemopexin in prime solution were important determinants for intra- and postoperative circulating scavenger protein resources.

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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
cardiopulmonary bypass, cell-free hemoglobin, congenital heart defect, haptoglobin, hemopexin, neonate
in
Journal of Clinical Medicine
volume
11
issue
14
article number
4071
publisher
MDPI AG
external identifiers
  • scopus:85137187069
  • pmid:35887835
ISSN
2077-0383
DOI
10.3390/jcm11144071
language
English
LU publication?
yes
id
264ee26b-882c-4971-af89-683988642bb5
date added to LUP
2022-11-11 16:10:08
date last changed
2024-05-02 18:24:46
@article{264ee26b-882c-4971-af89-683988642bb5,
  abstract     = {{<p>Exposure to circulating cell-free hemoglobin is a ubiquitous feature of open-heart surgery on cardiopulmonary bypass circulation. This study aims to determine the origins and dynamics of circulating cell-free hemoglobin and its major scavenger proteins haptoglobin and hemopexin during neonatal cardiopulmonary bypass. Forty neonates with an isolated critical congenital heart defect were included in a single-center prospective observational study. Blood samples were obtained preoperatively, hourly during bypass circulation, after bypass separation, at admission to the pediatric intensive care unit, and at postoperative days 1–3. Concentrations of cell-free hemoglobin, haptoglobin and hemopexin were determined using ELISA. Neonates were exposed to significantly elevated plasma concentrations of cell-free hemoglobin and a concomitant depletion of scavenger protein supplies during open-heart surgery. The main predictor of cell-free hemoglobin exposure was the concentration of cell-free hemoglobin in blood prime solution. Concentrations of haptoglobin and hemopexin in prime solution were important determinants for intra- and postoperative circulating scavenger protein resources.</p>}},
  author       = {{Jungner, Åsa and Vallius, Suvi and Gram, Magnus and Ley, David}},
  issn         = {{2077-0383}},
  keywords     = {{cardiopulmonary bypass; cell-free hemoglobin; congenital heart defect; haptoglobin; hemopexin; neonate}},
  language     = {{eng}},
  number       = {{14}},
  publisher    = {{MDPI AG}},
  series       = {{Journal of Clinical Medicine}},
  title        = {{Cell-Free Hemoglobin Concentration in Blood Prime Solution Is a Major Determinant of Cell-Free Hemoglobin Exposure during Cardiopulmonary Bypass Circulation in the Newborn}},
  url          = {{http://dx.doi.org/10.3390/jcm11144071}},
  doi          = {{10.3390/jcm11144071}},
  volume       = {{11}},
  year         = {{2022}},
}