Cell-Free Hemoglobin Concentration in Blood Prime Solution Is a Major Determinant of Cell-Free Hemoglobin Exposure during Cardiopulmonary Bypass Circulation in the Newborn
Jungner, Åsa LU ; Vallius, Suvi LU ; Gram, Magnus LU
and Ley, David
LU
(2022)
In Journal of Clinical Medicine
11(14).
- Abstract
Exposure to circulating cell-free hemoglobin is a ubiquitous feature of open-heart surgery on cardiopulmonary bypass circulation. This study aims to determine the origins and dynamics of circulating cell-free hemoglobin and its major scavenger proteins haptoglobin and hemopexin during neonatal cardiopulmonary bypass. Forty neonates with an isolated critical congenital heart defect were included in a single-center prospective observational study. Blood samples were obtained preoperatively, hourly during bypass circulation, after bypass separation, at admission to the pediatric intensive care unit, and at postoperative days 1–3. Concentrations of cell-free hemoglobin, haptoglobin and hemopexin were determined using ELISA. Neonates were... (More)
Exposure to circulating cell-free hemoglobin is a ubiquitous feature of open-heart surgery on cardiopulmonary bypass circulation. This study aims to determine the origins and dynamics of circulating cell-free hemoglobin and its major scavenger proteins haptoglobin and hemopexin during neonatal cardiopulmonary bypass. Forty neonates with an isolated critical congenital heart defect were included in a single-center prospective observational study. Blood samples were obtained preoperatively, hourly during bypass circulation, after bypass separation, at admission to the pediatric intensive care unit, and at postoperative days 1–3. Concentrations of cell-free hemoglobin, haptoglobin and hemopexin were determined using ELISA. Neonates were exposed to significantly elevated plasma concentrations of cell-free hemoglobin and a concomitant depletion of scavenger protein supplies during open-heart surgery. The main predictor of cell-free hemoglobin exposure was the concentration of cell-free hemoglobin in blood prime solution. Concentrations of haptoglobin and hemopexin in prime solution were important determinants for intra- and postoperative circulating scavenger protein resources.
(Less)
- author
- Jungner, Åsa
LU
; Vallius, Suvi
LU
; Gram, Magnus
LU
and Ley, David
LU
- organization
- publishing date
- 2022
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- cardiopulmonary bypass, cell-free hemoglobin, congenital heart defect, haptoglobin, hemopexin, neonate
- in
- Journal of Clinical Medicine
- volume
- 11
- issue
- 14
- article number
- 4071
- publisher
- MDPI AG
- external identifiers
-
- scopus:85137187069
- pmid:35887835
- ISSN
- 2077-0383
- DOI
- 10.3390/jcm11144071
- language
- English
- LU publication?
- yes
- id
- 264ee26b-882c-4971-af89-683988642bb5
- date added to LUP
- 2022-11-11 16:10:08
- date last changed
- 2024-05-30 21:30:34
@article{264ee26b-882c-4971-af89-683988642bb5,
abstract = {{<p>Exposure to circulating cell-free hemoglobin is a ubiquitous feature of open-heart surgery on cardiopulmonary bypass circulation. This study aims to determine the origins and dynamics of circulating cell-free hemoglobin and its major scavenger proteins haptoglobin and hemopexin during neonatal cardiopulmonary bypass. Forty neonates with an isolated critical congenital heart defect were included in a single-center prospective observational study. Blood samples were obtained preoperatively, hourly during bypass circulation, after bypass separation, at admission to the pediatric intensive care unit, and at postoperative days 1–3. Concentrations of cell-free hemoglobin, haptoglobin and hemopexin were determined using ELISA. Neonates were exposed to significantly elevated plasma concentrations of cell-free hemoglobin and a concomitant depletion of scavenger protein supplies during open-heart surgery. The main predictor of cell-free hemoglobin exposure was the concentration of cell-free hemoglobin in blood prime solution. Concentrations of haptoglobin and hemopexin in prime solution were important determinants for intra- and postoperative circulating scavenger protein resources.</p>}},
author = {{Jungner, Åsa and Vallius, Suvi and Gram, Magnus and Ley, David}},
issn = {{2077-0383}},
keywords = {{cardiopulmonary bypass; cell-free hemoglobin; congenital heart defect; haptoglobin; hemopexin; neonate}},
language = {{eng}},
number = {{14}},
publisher = {{MDPI AG}},
series = {{Journal of Clinical Medicine}},
title = {{Cell-Free Hemoglobin Concentration in Blood Prime Solution Is a Major Determinant of Cell-Free Hemoglobin Exposure during Cardiopulmonary Bypass Circulation in the Newborn}},
url = {{http://dx.doi.org/10.3390/jcm11144071}},
doi = {{10.3390/jcm11144071}},
volume = {{11}},
year = {{2022}},
}