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Effect of vitamin E on autolysis and sporulation of Aspergillus nidulans

Emri, T; Molnar, Z; Pusztahelyi, T; Rosén, Stefan LU and Pocsi, I (2004) In Applied Biochemistry and Biotechnology 118(1-3). p.337-348
Abstract
The morphologic and physiologic effects of vitamin E, a powerful antioxidant, on the autolysis and sporulation of Aspergillus nidulans FGSC26 were studied. In carbon-depleted submerged cultures, reactive oxygen species (ROS) accumulated in the cells and, concomitantly, progressing autolysis was observed, which was characterized by decreasing dry cell masses and pellet diameters as well as by increasing extracellular chitinase activities. Vitamin E supplemented at a concentration of 1g/L hindered effectively the intracellular accumulation of ROS, the autolytic loss of biomass, the disintegration of pellets, and the release of chitinase activities. In surface cultures, vitamin E inhibited autolysis of both A. nidulans FGSC26 and a... (More)
The morphologic and physiologic effects of vitamin E, a powerful antioxidant, on the autolysis and sporulation of Aspergillus nidulans FGSC26 were studied. In carbon-depleted submerged cultures, reactive oxygen species (ROS) accumulated in the cells and, concomitantly, progressing autolysis was observed, which was characterized by decreasing dry cell masses and pellet diameters as well as by increasing extracellular chitinase activities. Vitamin E supplemented at a concentration of 1g/L hindered effectively the intracellular accumulation of ROS, the autolytic loss of biomass, the disintegration of pellets, and the release of chitinase activities. In surface cultures, vitamin E inhibited autolysis of both A. nidulans FGSC26 and a loss-of-function FlbA autolytic phenotype mutant. In addition, supplementation of the culture medium with this antioxidant also had a negative effect on the sporulation of strain FGSC26 and the FadA(G203R) hypersporulating phenotype mutant. These results suggest that accumulation of ROS was involved in the initiation of both sporulation and autolysis in this filamentous fungus, but that FadA/FlbA signaling was not involved in this vitamin E-dependent regulation. Vitamin E can be recommended as a supplement in fermentations in which the disintegration of pellets and gross autolysis should be avoided. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
flbA, fadA, autolysis, sporulation, menadione, Aspergillus nidulans, vitamin E
in
Applied Biochemistry and Biotechnology
volume
118
issue
1-3
pages
337 - 348
publisher
Humana Press
external identifiers
  • wos:000223278500029
  • scopus:4344693860
ISSN
1559-0291
language
English
LU publication?
yes
id
936c579e-c8e7-4300-827d-5cd5e5257abb (old id 270997)
date added to LUP
2007-10-22 08:26:58
date last changed
2017-01-01 05:14:27
@article{936c579e-c8e7-4300-827d-5cd5e5257abb,
  abstract     = {The morphologic and physiologic effects of vitamin E, a powerful antioxidant, on the autolysis and sporulation of Aspergillus nidulans FGSC26 were studied. In carbon-depleted submerged cultures, reactive oxygen species (ROS) accumulated in the cells and, concomitantly, progressing autolysis was observed, which was characterized by decreasing dry cell masses and pellet diameters as well as by increasing extracellular chitinase activities. Vitamin E supplemented at a concentration of 1g/L hindered effectively the intracellular accumulation of ROS, the autolytic loss of biomass, the disintegration of pellets, and the release of chitinase activities. In surface cultures, vitamin E inhibited autolysis of both A. nidulans FGSC26 and a loss-of-function FlbA autolytic phenotype mutant. In addition, supplementation of the culture medium with this antioxidant also had a negative effect on the sporulation of strain FGSC26 and the FadA(G203R) hypersporulating phenotype mutant. These results suggest that accumulation of ROS was involved in the initiation of both sporulation and autolysis in this filamentous fungus, but that FadA/FlbA signaling was not involved in this vitamin E-dependent regulation. Vitamin E can be recommended as a supplement in fermentations in which the disintegration of pellets and gross autolysis should be avoided.},
  author       = {Emri, T and Molnar, Z and Pusztahelyi, T and Rosén, Stefan and Pocsi, I},
  issn         = {1559-0291},
  keyword      = {flbA,fadA,autolysis,sporulation,menadione,Aspergillus nidulans,vitamin E},
  language     = {eng},
  number       = {1-3},
  pages        = {337--348},
  publisher    = {Humana Press},
  series       = {Applied Biochemistry and Biotechnology},
  title        = {Effect of vitamin E on autolysis and sporulation of Aspergillus nidulans},
  volume       = {118},
  year         = {2004},
}