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Influence of fadA(G203R) and Delta flba mutations on morphology and physiology of submerged Aspergillus nidulans cultures

Molnar, Z; Meszaros, E; Szilagyi, Z; Rosén, Stefan LU ; Emri, T and Pocsi, I (2004) In Applied Biochemistry and Biotechnology 118(1-3). p.349-360
Abstract
Morphologic and physiologic changes taking place in carbon-limited submerged cultures of Aspergillus nidulans DeltaflbA and fadA(G203R) strains were studied. Loss-of-function mutation of the flbA gene resulted in an altered germination with unusually thick germination tubes, "fluffy" pellet morphology, as well as a reduced fragmentation rate of hyphae during autolysis. In the fadA(G203R) mutant strain, conidiophores formed in the stationary phase of growth, and the size of pellets shrank considerably. There were no significant differences in the generation of reactive oxygen species (ROS) and in the specific catalase and superoxide dismutase activities by the tested mutants and the appropriate parental strains. Therefore, the participation... (More)
Morphologic and physiologic changes taking place in carbon-limited submerged cultures of Aspergillus nidulans DeltaflbA and fadA(G203R) strains were studied. Loss-of-function mutation of the flbA gene resulted in an altered germination with unusually thick germination tubes, "fluffy" pellet morphology, as well as a reduced fragmentation rate of hyphae during autolysis. In the fadA(G203R) mutant strain, conidiophores formed in the stationary phase of growth, and the size of pellets shrank considerably. There were no significant differences in the generation of reactive oxygen species (ROS) and in the specific catalase and superoxide dismutase activities by the tested mutants and the appropriate parental strains. Therefore, the participation of ROS or antioxidative enzymes in FadA/FlbA signaling pathways seems to be unlikely in submerged cultures. On the other hand, earlier increases in the extracellular-protease and ammonia production were recorded with the DeltaflbA strain, whereas the protease and ammonia production of the fadA(G203R) mutant lagged behind those of the wild-type strains. Similar changes in the time courses of the induction of gamma-glutamyltranspeptidase and the degradation of glutathione were observed. These results suggest that FadA/FlbA signaling may be involved in the mobilization of protein and peptide reserves as energy sources during carbon starvation. (Less)
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author
organization
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Contribution to journal
publication status
published
subject
keywords
autolysis, flbA, Aspergillus nidulans, fadA, chitinase, glutathione
in
Applied Biochemistry and Biotechnology
volume
118
issue
1-3
pages
349 - 360
publisher
Humana Press
external identifiers
  • wos:000223278500030
  • scopus:4344716671
ISSN
1559-0291
language
English
LU publication?
yes
id
dfa5bbce-6ed9-48fd-b21d-ab2a6d42513d (old id 271003)
alternative location
http://www.ingentaconnect.com/content/hum/abab/2004/00000118/F0030001/art00029
date added to LUP
2007-10-28 15:35:30
date last changed
2017-03-12 03:40:16
@article{dfa5bbce-6ed9-48fd-b21d-ab2a6d42513d,
  abstract     = {Morphologic and physiologic changes taking place in carbon-limited submerged cultures of Aspergillus nidulans DeltaflbA and fadA(G203R) strains were studied. Loss-of-function mutation of the flbA gene resulted in an altered germination with unusually thick germination tubes, "fluffy" pellet morphology, as well as a reduced fragmentation rate of hyphae during autolysis. In the fadA(G203R) mutant strain, conidiophores formed in the stationary phase of growth, and the size of pellets shrank considerably. There were no significant differences in the generation of reactive oxygen species (ROS) and in the specific catalase and superoxide dismutase activities by the tested mutants and the appropriate parental strains. Therefore, the participation of ROS or antioxidative enzymes in FadA/FlbA signaling pathways seems to be unlikely in submerged cultures. On the other hand, earlier increases in the extracellular-protease and ammonia production were recorded with the DeltaflbA strain, whereas the protease and ammonia production of the fadA(G203R) mutant lagged behind those of the wild-type strains. Similar changes in the time courses of the induction of gamma-glutamyltranspeptidase and the degradation of glutathione were observed. These results suggest that FadA/FlbA signaling may be involved in the mobilization of protein and peptide reserves as energy sources during carbon starvation.},
  author       = {Molnar, Z and Meszaros, E and Szilagyi, Z and Rosén, Stefan and Emri, T and Pocsi, I},
  issn         = {1559-0291},
  keyword      = {autolysis,flbA,Aspergillus nidulans,fadA,chitinase,glutathione},
  language     = {eng},
  number       = {1-3},
  pages        = {349--360},
  publisher    = {Humana Press},
  series       = {Applied Biochemistry and Biotechnology},
  title        = {Influence of fadA(G203R) and Delta flba mutations on morphology and physiology of submerged Aspergillus nidulans cultures},
  volume       = {118},
  year         = {2004},
}