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Stimulation of distal airspace fluid clearance in guinea pigs involves bumetanide-sensitive ion transport

Ye, X; Norlin, A and Folkesson, Hans LU (2004) In American Journal of Obstetrics and Gynecology 191(1). p.340-345
Abstract
Objective: This study was undertaken to test the hypothesis that beta-adrenoceptor stimulation of fetal lung fluid absorption in near-term guinea pig fetuses involves bumetanide-sensitive ion transport. Study design: Fetuses were obtained from timed-pregnant guinea pigs at 61 to 69 days' gestation with and without oxytocin-induced preterm labor. The fetuses were placed on continuous positive airway pressure oxygenation, and an isosmolar 5% albumin solution was instilled into the lungs. Distal airspace fluid clearance was measured over 1 hour from the increase in distal airspace protein concentration as fluid was reabsorbed with and without the Cl- transport inhibitor bumetanide. Results: Fetal lungs began to absorb distal airspace fluid at... (More)
Objective: This study was undertaken to test the hypothesis that beta-adrenoceptor stimulation of fetal lung fluid absorption in near-term guinea pig fetuses involves bumetanide-sensitive ion transport. Study design: Fetuses were obtained from timed-pregnant guinea pigs at 61 to 69 days' gestation with and without oxytocin-induced preterm labor. The fetuses were placed on continuous positive airway pressure oxygenation, and an isosmolar 5% albumin solution was instilled into the lungs. Distal airspace fluid clearance was measured over 1 hour from the increase in distal airspace protein concentration as fluid was reabsorbed with and without the Cl- transport inhibitor bumetanide. Results: Fetal lungs began to absorb distal airspace fluid at 64 to 66 days' gestation, and at birth, distal airspace fluid clearance rapidly quadrupled. Labor induction by oxytocin stimulated distal airspace fluid clearance. Distal airspace fluid clearance, when present, was sensitive to propranolol-inhibition and depended on beta-adrenoceptor stimulation. Fluid secretion at 61 days' gestation was reduced by bumetanide instillation. Bumetanide addition was only inhibitory when distal airspace fluid clearance was propranolol-sensitive. Conclusion: beta-adrenoceptor stimulation from endogenous fetal epinephrine increased fetal distal airspace fluid clearance and involved bumetanide-sensitive ion transport. (C) 2004 Elsevier Inc. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cl- transport, bumetanide, alveolar epithelium, beta-adrenoceptors, epinephrine perinatal development
in
American Journal of Obstetrics and Gynecology
volume
191
issue
1
pages
340 - 345
publisher
Elsevier
external identifiers
  • pmid:15295389
  • wos:000223139100055
  • scopus:4043123374
ISSN
1097-6868
DOI
10.1016/j.ajog.2003.09.074
language
English
LU publication?
yes
id
9928d242-86b9-4a92-b5c1-8fb26911eb5c (old id 271036)
date added to LUP
2007-11-07 08:18:44
date last changed
2017-01-01 04:19:49
@article{9928d242-86b9-4a92-b5c1-8fb26911eb5c,
  abstract     = {Objective: This study was undertaken to test the hypothesis that beta-adrenoceptor stimulation of fetal lung fluid absorption in near-term guinea pig fetuses involves bumetanide-sensitive ion transport. Study design: Fetuses were obtained from timed-pregnant guinea pigs at 61 to 69 days' gestation with and without oxytocin-induced preterm labor. The fetuses were placed on continuous positive airway pressure oxygenation, and an isosmolar 5% albumin solution was instilled into the lungs. Distal airspace fluid clearance was measured over 1 hour from the increase in distal airspace protein concentration as fluid was reabsorbed with and without the Cl- transport inhibitor bumetanide. Results: Fetal lungs began to absorb distal airspace fluid at 64 to 66 days' gestation, and at birth, distal airspace fluid clearance rapidly quadrupled. Labor induction by oxytocin stimulated distal airspace fluid clearance. Distal airspace fluid clearance, when present, was sensitive to propranolol-inhibition and depended on beta-adrenoceptor stimulation. Fluid secretion at 61 days' gestation was reduced by bumetanide instillation. Bumetanide addition was only inhibitory when distal airspace fluid clearance was propranolol-sensitive. Conclusion: beta-adrenoceptor stimulation from endogenous fetal epinephrine increased fetal distal airspace fluid clearance and involved bumetanide-sensitive ion transport. (C) 2004 Elsevier Inc. All rights reserved.},
  author       = {Ye, X and Norlin, A and Folkesson, Hans},
  issn         = {1097-6868},
  keyword      = {Cl- transport,bumetanide,alveolar epithelium,beta-adrenoceptors,epinephrine perinatal development},
  language     = {eng},
  number       = {1},
  pages        = {340--345},
  publisher    = {Elsevier},
  series       = {American Journal of Obstetrics and Gynecology},
  title        = {Stimulation of distal airspace fluid clearance in guinea pigs involves bumetanide-sensitive ion transport},
  url          = {http://dx.doi.org/10.1016/j.ajog.2003.09.074},
  volume       = {191},
  year         = {2004},
}