Penetration of aluminium salts through pig skin in vitro
(2012) 11th Congress of the European Society of Contact Dermatitis (ESCD) In Contact Dermatitis 66(s2). p.52-52- Abstract
- Background: Recently it was suggested to use aluminium chloride hexahydrate in petrolatum at 10% to detect aluminium contact allergy. Patch testing with aluminium chloride hexahydrate at 20% did not demonstrate a higher number of positive test reactions. It was speculated that the constricting effect of aluminium salts might impair skin penetration. Objectives: The purpose of the present study is to estimate the concentration of aluminium in various skin layers and in the recipient phase using different aluminium test preparations on pig skin in an in vitro diffusion cell system. Methods: Full-thickness (7sim; porcine ear skin was mounted in Franz-type diffusion cells. The diffusional area was 0.64 cm2 and recipient volume 4 ml. The dermal... (More)
- Background: Recently it was suggested to use aluminium chloride hexahydrate in petrolatum at 10% to detect aluminium contact allergy. Patch testing with aluminium chloride hexahydrate at 20% did not demonstrate a higher number of positive test reactions. It was speculated that the constricting effect of aluminium salts might impair skin penetration. Objectives: The purpose of the present study is to estimate the concentration of aluminium in various skin layers and in the recipient phase using different aluminium test preparations on pig skin in an in vitro diffusion cell system. Methods: Full-thickness (7sim; porcine ear skin was mounted in Franz-type diffusion cells. The diffusional area was 0.64 cm2 and recipient volume 4 ml. The dermal side was exposed to a recipient solution consisting of PBS (phosphate buffer saline, pH 7.4). Aluminium chloride hexahydrate preparations in petrolatum and in water at 10% were applied to the donor compartment facing the epidermis for 24 hr. All samples were analysed for total aluminium by atomic absorption spectrometry. The limit of detection was 0.01 μg/ml. Results: No aluminium could be detected in the recipient phase in the six diffusion cells. Conclusions: Neither aluminium in petrolatum nor in water gave detectable amounts of penetrating aluminium in 24 h. The lipophilicity of the vehicle was not decisive. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2717cc84-b048-4695-8950-55e17f192f04
- author
- Siemund, I. LU ; Bergendorff, O. LU ; Zimerson, E. LU ; Persson, Christina LU ; Hindsén, M. LU and Bruze, M. LU
- organization
- publishing date
- 2012-06-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- aluminum salt, aluminum, petrolatum, aluminum chloride, water, sodium chloride, phosphate, pig, skin, in vitro study, society, contact dermatitis, recipient, diffusion, lipophilicity, atomic absorption spectrometry, epidermis, skin penetration, donor, pH, limit of detection, ear, thickness, contact allergy
- in
- Contact Dermatitis
- volume
- 66
- issue
- s2
- pages
- 52 - 52
- publisher
- Wiley-Blackwell
- conference name
- 11th Congress of the European Society of Contact Dermatitis (ESCD)
- conference location
- Malmö, Sweden
- conference dates
- 2012-06-13 - 2012-06-16
- ISSN
- 0105-1873
- DOI
- 10.1111/j.1600-0536.2012.02111.x
- language
- English
- LU publication?
- yes
- id
- 2717cc84-b048-4695-8950-55e17f192f04
- date added to LUP
- 2017-06-15 14:18:06
- date last changed
- 2018-11-21 21:32:49
@misc{2717cc84-b048-4695-8950-55e17f192f04,
abstract = {{Background: Recently it was suggested to use aluminium chloride hexahydrate in petrolatum at 10% to detect aluminium contact allergy. Patch testing with aluminium chloride hexahydrate at 20% did not demonstrate a higher number of positive test reactions. It was speculated that the constricting effect of aluminium salts might impair skin penetration. Objectives: The purpose of the present study is to estimate the concentration of aluminium in various skin layers and in the recipient phase using different aluminium test preparations on pig skin in an in vitro diffusion cell system. Methods: Full-thickness (7sim; porcine ear skin was mounted in Franz-type diffusion cells. The diffusional area was 0.64 cm2 and recipient volume 4 ml. The dermal side was exposed to a recipient solution consisting of PBS (phosphate buffer saline, pH 7.4). Aluminium chloride hexahydrate preparations in petrolatum and in water at 10% were applied to the donor compartment facing the epidermis for 24 hr. All samples were analysed for total aluminium by atomic absorption spectrometry. The limit of detection was 0.01 μg/ml. Results: No aluminium could be detected in the recipient phase in the six diffusion cells. Conclusions: Neither aluminium in petrolatum nor in water gave detectable amounts of penetrating aluminium in 24 h. The lipophilicity of the vehicle was not decisive.}},
author = {{Siemund, I. and Bergendorff, O. and Zimerson, E. and Persson, Christina and Hindsén, M. and Bruze, M.}},
issn = {{0105-1873}},
keywords = {{aluminum salt; aluminum; petrolatum; aluminum chloride; water; sodium chloride; phosphate; pig; skin; in vitro study; society; contact dermatitis; recipient; diffusion; lipophilicity; atomic absorption spectrometry; epidermis; skin penetration; donor; pH; limit of detection; ear; thickness; contact allergy}},
language = {{eng}},
month = {{06}},
note = {{Conference Abstract}},
number = {{s2}},
pages = {{52--52}},
publisher = {{Wiley-Blackwell}},
series = {{Contact Dermatitis}},
title = {{Penetration of aluminium salts through pig skin in vitro}},
url = {{http://dx.doi.org/10.1111/j.1600-0536.2012.02111.x}},
doi = {{10.1111/j.1600-0536.2012.02111.x}},
volume = {{66}},
year = {{2012}},
}