Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Generation of human iPS cell line CTL07-II from human fibroblasts, under defined and xeno-free conditions

Kele, Malin ; Day, Kelly ; Rönnholm, Harriet ; Schuster, Jens ; Dahl, Niklas and Falk, Anna LU (2016) In Stem Cell Research 17(3). p.474-478
Abstract

CTL07-II is a healthy feeder-free and characterized human induced pluripotent stem (iPS) cell line. Cultured under xeno-free and defined conditions. The line is generated from healthy human fibroblasts with non-integrating Sendai virus vectors encoding the four Yamanaka factors, OCT4, SOX2, KLF4 and cMYC. The generated iPS cells are free from reprogramming vectors and their purity, karyotypic stability and pluripotent capacity is confirmed.

Please use this url to cite or link to this publication:
author
; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cell Differentiation, Cell Line, Cellular Reprogramming, Culture Media/chemistry, Embryoid Bodies/cytology, Fibroblasts/cytology, Genetic Vectors/genetics, Humans, Induced Pluripotent Stem Cells/cytology, Karyotype, Male, Microscopy, Fluorescence, Sendai virus/genetics, Transcription Factors/genetics
in
Stem Cell Research
volume
17
issue
3
pages
5 pages
publisher
Elsevier
external identifiers
  • pmid:27789397
  • scopus:85024376878
ISSN
1876-7753
DOI
10.1016/j.scr.2016.09.028
language
English
LU publication?
no
id
2727a117-88b8-40f1-a6d0-51d54cefd528
date added to LUP
2021-08-10 13:01:04
date last changed
2024-09-22 22:39:17
@article{2727a117-88b8-40f1-a6d0-51d54cefd528,
  abstract     = {{<p>CTL07-II is a healthy feeder-free and characterized human induced pluripotent stem (iPS) cell line. Cultured under xeno-free and defined conditions. The line is generated from healthy human fibroblasts with non-integrating Sendai virus vectors encoding the four Yamanaka factors, OCT4, SOX2, KLF4 and cMYC. The generated iPS cells are free from reprogramming vectors and their purity, karyotypic stability and pluripotent capacity is confirmed.</p>}},
  author       = {{Kele, Malin and Day, Kelly and Rönnholm, Harriet and Schuster, Jens and Dahl, Niklas and Falk, Anna}},
  issn         = {{1876-7753}},
  keywords     = {{Cell Differentiation; Cell Line; Cellular Reprogramming; Culture Media/chemistry; Embryoid Bodies/cytology; Fibroblasts/cytology; Genetic Vectors/genetics; Humans; Induced Pluripotent Stem Cells/cytology; Karyotype; Male; Microscopy, Fluorescence; Sendai virus/genetics; Transcription Factors/genetics}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{474--478}},
  publisher    = {{Elsevier}},
  series       = {{Stem Cell Research}},
  title        = {{Generation of human iPS cell line CTL07-II from human fibroblasts, under defined and xeno-free conditions}},
  url          = {{https://lup.lub.lu.se/search/files/101075400/Generation_of_human_iPS_cell_line.pdf}},
  doi          = {{10.1016/j.scr.2016.09.028}},
  volume       = {{17}},
  year         = {{2016}},
}