Ultrasonic-trap-enhanced selectivity in capillary electrophoresis.
(2003) In Ultrasonics 41(4). p.329-333- Abstract
- We combine ultrasonic trapping and capillary electrophoresis (CE) with the goal to detect ultra-low concentrations of proteins via size-selective separation and enrichment of antibody-coated latex spheres. An 8.5 MHz standing ultrasonic wave is longitudinally coupled into the sub-100-small mu, Greekm diam capillary of the CE system. Competition between acoustic and viscous forces result in in-flow separation of small mu, Greekm-diam spheres according to their size. Experiments separating 2.8- and 2.1-small mu, Greekm-diam fluorescent latex particles, which model a protein-specific immunocomplex/free particle mixture, indicate a potential improvement of the concentration limit of detection of 104 compared to current CE systems. Theoretical... (More)
- We combine ultrasonic trapping and capillary electrophoresis (CE) with the goal to detect ultra-low concentrations of proteins via size-selective separation and enrichment of antibody-coated latex spheres. An 8.5 MHz standing ultrasonic wave is longitudinally coupled into the sub-100-small mu, Greekm diam capillary of the CE system. Competition between acoustic and viscous forces result in in-flow separation of small mu, Greekm-diam spheres according to their size. Experiments separating 2.8- and 2.1-small mu, Greekm-diam fluorescent latex particles, which model a protein-specific immunocomplex/free particle mixture, indicate a potential improvement of the concentration limit of detection of 104 compared to current CE systems. Theoretical calculations show room for further improvement. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/132765
- author
- Wiklund, M ; Spégel, Peter LU ; Nilsson, Staffan LU and Hertz, H M
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Ultrasonic trapping, Capillary electrophoresis, Particle separation, Concentration limit of detection
- in
- Ultrasonics
- volume
- 41
- issue
- 4
- pages
- 329 - 333
- publisher
- Elsevier
- external identifiers
-
- wos:000183679100015
- scopus:0038182656
- ISSN
- 0041-624X
- DOI
- 10.1016/S0041-624X(02)00460-2
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
- id
- 27369816-d6b4-4e79-8f22-c33ad4503d35 (old id 132765)
- date added to LUP
- 2016-04-01 16:50:25
- date last changed
- 2022-01-28 22:33:55
@article{27369816-d6b4-4e79-8f22-c33ad4503d35,
abstract = {{We combine ultrasonic trapping and capillary electrophoresis (CE) with the goal to detect ultra-low concentrations of proteins via size-selective separation and enrichment of antibody-coated latex spheres. An 8.5 MHz standing ultrasonic wave is longitudinally coupled into the sub-100-small mu, Greekm diam capillary of the CE system. Competition between acoustic and viscous forces result in in-flow separation of small mu, Greekm-diam spheres according to their size. Experiments separating 2.8- and 2.1-small mu, Greekm-diam fluorescent latex particles, which model a protein-specific immunocomplex/free particle mixture, indicate a potential improvement of the concentration limit of detection of 104 compared to current CE systems. Theoretical calculations show room for further improvement.}},
author = {{Wiklund, M and Spégel, Peter and Nilsson, Staffan and Hertz, H M}},
issn = {{0041-624X}},
keywords = {{Ultrasonic trapping; Capillary electrophoresis; Particle separation; Concentration limit of detection}},
language = {{eng}},
number = {{4}},
pages = {{329--333}},
publisher = {{Elsevier}},
series = {{Ultrasonics}},
title = {{Ultrasonic-trap-enhanced selectivity in capillary electrophoresis.}},
url = {{http://dx.doi.org/10.1016/S0041-624X(02)00460-2}},
doi = {{10.1016/S0041-624X(02)00460-2}},
volume = {{41}},
year = {{2003}},
}