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The GDP-GTP exchange factor collybistin: An essential determinant of neuronal gephyrin clustering

Harvey, K; Duguid, IC; Alldred, MJ; Beatty, SE; Ward, H; Keep, NH; Lingenfelter, SE; Pearce, BR; Lundgren, Johan LU and Owen, MJ, et al. (2004) In Journal of Neuroscience 24(25). p.5816-5826
Abstract
Glycine receptors (GlyRs) and specific subtypes of GABA(A) receptors are clustered at synapses by the multidomain protein gephyrin, which in turn is translocated to the cell membrane by the GDP-GTP exchange factor collybistin. We report the characterization of several new variants of collybistin, which are created by alternative splicing of exons encoding an N-terminal src homology 3 (SH3) domain and three alternate C termini (CB1, CB2, and CB3). The presence of the SH3 domain negatively regulates the ability of collybistin to translocate gephyrin to submembrane microaggregates in transfected mammalian cells. Because the majority of native collybistin isoforms appear to harbor the SH3 domain, this suggests that collybistin activity may be... (More)
Glycine receptors (GlyRs) and specific subtypes of GABA(A) receptors are clustered at synapses by the multidomain protein gephyrin, which in turn is translocated to the cell membrane by the GDP-GTP exchange factor collybistin. We report the characterization of several new variants of collybistin, which are created by alternative splicing of exons encoding an N-terminal src homology 3 (SH3) domain and three alternate C termini (CB1, CB2, and CB3). The presence of the SH3 domain negatively regulates the ability of collybistin to translocate gephyrin to submembrane microaggregates in transfected mammalian cells. Because the majority of native collybistin isoforms appear to harbor the SH3 domain, this suggests that collybistin activity may be regulated by protein-protein interactions at the SH3 domain. We localized the binding sites for collybistin and the GlyR beta subunit to the C-terminal MoeA homology domain of gephyrin and show that multimerization of this domain is required for collybistin-gephyrin and GlyR-gephyrin interactions. We also demonstrate that gephyrin clustering in recombinant systems and cultured neurons requires both collybistin-gephyrin interactions and an intact collybistin pleckstrin homology domain. The vital importance of collybistin for inhibitory synaptogenesis is underlined by the discovery of a mutation (G55A) in exon 2 of the human collybistin gene (ARHGEF9) in a patient with clinical symptoms of both hyperekplexia and epilepsy. The clinical manifestation of this collybistin missense mutation may result, at least in part, from mislocalization of gephyrin and a major GABA(A) receptor subtype. (Less)
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keywords
hyperekplexia, glycine receptor, GABA(A) receptor, dendritic transport, epilepsy, trafficking
in
Journal of Neuroscience
volume
24
issue
25
pages
5816 - 5826
publisher
Society for Neuroscience
external identifiers
  • pmid:15215304
  • wos:000222237900018
  • scopus:3042774411
ISSN
1529-2401
DOI
10.1523/JNEUROSCI.1184-04.2004
language
English
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yes
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90a15f20-330c-4f69-8e20-8fa0b50390dc (old id 273787)
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http://www.ncbi.nlm.nih.gov/pubmed/15215304
http://www.jneurosci.org/content/24/25/5816.long
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2007-10-22 14:56:03
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2017-10-22 04:46:15
@article{90a15f20-330c-4f69-8e20-8fa0b50390dc,
  abstract     = {Glycine receptors (GlyRs) and specific subtypes of GABA(A) receptors are clustered at synapses by the multidomain protein gephyrin, which in turn is translocated to the cell membrane by the GDP-GTP exchange factor collybistin. We report the characterization of several new variants of collybistin, which are created by alternative splicing of exons encoding an N-terminal src homology 3 (SH3) domain and three alternate C termini (CB1, CB2, and CB3). The presence of the SH3 domain negatively regulates the ability of collybistin to translocate gephyrin to submembrane microaggregates in transfected mammalian cells. Because the majority of native collybistin isoforms appear to harbor the SH3 domain, this suggests that collybistin activity may be regulated by protein-protein interactions at the SH3 domain. We localized the binding sites for collybistin and the GlyR beta subunit to the C-terminal MoeA homology domain of gephyrin and show that multimerization of this domain is required for collybistin-gephyrin and GlyR-gephyrin interactions. We also demonstrate that gephyrin clustering in recombinant systems and cultured neurons requires both collybistin-gephyrin interactions and an intact collybistin pleckstrin homology domain. The vital importance of collybistin for inhibitory synaptogenesis is underlined by the discovery of a mutation (G55A) in exon 2 of the human collybistin gene (ARHGEF9) in a patient with clinical symptoms of both hyperekplexia and epilepsy. The clinical manifestation of this collybistin missense mutation may result, at least in part, from mislocalization of gephyrin and a major GABA(A) receptor subtype.},
  author       = {Harvey, K and Duguid, IC and Alldred, MJ and Beatty, SE and Ward, H and Keep, NH and Lingenfelter, SE and Pearce, BR and Lundgren, Johan and Owen, MJ and Smart, TG and Luscher, B and Rees, MI and Harvey, RJ},
  issn         = {1529-2401},
  keyword      = {hyperekplexia,glycine receptor,GABA(A) receptor,dendritic transport,epilepsy,trafficking},
  language     = {eng},
  number       = {25},
  pages        = {5816--5826},
  publisher    = {Society for Neuroscience},
  series       = {Journal of Neuroscience},
  title        = {The GDP-GTP exchange factor collybistin: An essential determinant of neuronal gephyrin clustering},
  url          = {http://dx.doi.org/10.1523/JNEUROSCI.1184-04.2004},
  volume       = {24},
  year         = {2004},
}