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The constant proportion of grana and stroma lamellae in plant chloroplasts

Albertsson, Per-Åke LU and Andreasson, E (2004) In Physiologia Plantarum 121(2). p.334-342
Abstract
The relative proportion of stroma lamellae and grana end membranes was determined from electron micrographs of 58 chloroplasts from 21 different plant species. The percentage of grana end membranes varied between 1 and 21% of the total thylakoid membrane indicating a large variation in the size of grana stacks. By contrast the stroma lamellae account for 20.3 +/- 2.5 (SD)% of the total thylakoid membrane. A plot of percentage stroma lamellae against percentage of grana end membranes fits a straight line with a slope of zero showing that the proportion of stroma lamellae is independent of the size of the grana stacks. That stroma lamellae account for about 20% of the thylakoid membrane is in agreement with fragmentation and separation... (More)
The relative proportion of stroma lamellae and grana end membranes was determined from electron micrographs of 58 chloroplasts from 21 different plant species. The percentage of grana end membranes varied between 1 and 21% of the total thylakoid membrane indicating a large variation in the size of grana stacks. By contrast the stroma lamellae account for 20.3 +/- 2.5 (SD)% of the total thylakoid membrane. A plot of percentage stroma lamellae against percentage of grana end membranes fits a straight line with a slope of zero showing that the proportion of stroma lamellae is independent of the size of the grana stacks. That stroma lamellae account for about 20% of the thylakoid membrane is in agreement with fragmentation and separation analysis (Gadjieva et al. Biochim. Biophys. Acta 144: 92-100, 1999). Chloroplasts from spinach, grown under high or low light, were fragmented by sonication and separated by countercurrent distribution into two vesicle populations originating from grana and stroma lamellae plus end membranes, respectively. The separation diagrams were very similar lending independent support for the notion that the proportion of stroma lamellae is constant. The results are discussed in relation to the composition and function of the chloroplast in plants grown under different environmental conditions, and in relation to a recent quantitative model for the thylakoid (Albertsson, Trends Plant Sci. 6: 349-354, 2001). (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Physiologia Plantarum
volume
121
issue
2
pages
334 - 342
publisher
Wiley Online Library
external identifiers
  • wos:000221439000019
ISSN
0031-9317
DOI
10.1111/j.1399-3054.2004.00315.x
language
English
LU publication?
yes
id
aa135bad-de1f-42a7-9ee6-8b5b5a8d0011 (old id 277505)
date added to LUP
2007-09-03 10:41:28
date last changed
2016-04-15 19:05:59
@article{aa135bad-de1f-42a7-9ee6-8b5b5a8d0011,
  abstract     = {The relative proportion of stroma lamellae and grana end membranes was determined from electron micrographs of 58 chloroplasts from 21 different plant species. The percentage of grana end membranes varied between 1 and 21% of the total thylakoid membrane indicating a large variation in the size of grana stacks. By contrast the stroma lamellae account for 20.3 +/- 2.5 (SD)% of the total thylakoid membrane. A plot of percentage stroma lamellae against percentage of grana end membranes fits a straight line with a slope of zero showing that the proportion of stroma lamellae is independent of the size of the grana stacks. That stroma lamellae account for about 20% of the thylakoid membrane is in agreement with fragmentation and separation analysis (Gadjieva et al. Biochim. Biophys. Acta 144: 92-100, 1999). Chloroplasts from spinach, grown under high or low light, were fragmented by sonication and separated by countercurrent distribution into two vesicle populations originating from grana and stroma lamellae plus end membranes, respectively. The separation diagrams were very similar lending independent support for the notion that the proportion of stroma lamellae is constant. The results are discussed in relation to the composition and function of the chloroplast in plants grown under different environmental conditions, and in relation to a recent quantitative model for the thylakoid (Albertsson, Trends Plant Sci. 6: 349-354, 2001).},
  author       = {Albertsson, Per-Åke and Andreasson, E},
  issn         = {0031-9317},
  language     = {eng},
  number       = {2},
  pages        = {334--342},
  publisher    = {Wiley Online Library},
  series       = {Physiologia Plantarum},
  title        = {The constant proportion of grana and stroma lamellae in plant chloroplasts},
  url          = {http://dx.doi.org/10.1111/j.1399-3054.2004.00315.x},
  volume       = {121},
  year         = {2004},
}