Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Structural basis for PoxtA-mediated resistance to phenicol and oxazolidinone antibiotics

Crowe-McAuliffe, Caillan ; Murina, Victoriia ; Turnbull, Kathryn Jane ; Huch, Susanne ; Kasari, Marje ; Takada, Hiraku ; Nersisyan, Lilit ; Sundsfjord, Arnfinn ; Hegstad, Kristin and Atkinson, Gemma C LU , et al. (2022) In Nature Communications 13.
Abstract

PoxtA and OptrA are ATP binding cassette (ABC) proteins of the F subtype (ABCF). They confer resistance to oxazolidinone and phenicol antibiotics, such as linezolid and chloramphenicol, which stall translating ribosomes when certain amino acids are present at a defined position in the nascent polypeptide chain. These proteins are often encoded on mobile genetic elements, facilitating their rapid spread amongst Gram-positive bacteria, and are thought to confer resistance by binding to the ribosome and dislodging the bound antibiotic. However, the mechanistic basis of this resistance remains unclear. Here we refine the PoxtA spectrum of action, demonstrate alleviation of linezolid-induced context-dependent translational stalling, and... (More)

PoxtA and OptrA are ATP binding cassette (ABC) proteins of the F subtype (ABCF). They confer resistance to oxazolidinone and phenicol antibiotics, such as linezolid and chloramphenicol, which stall translating ribosomes when certain amino acids are present at a defined position in the nascent polypeptide chain. These proteins are often encoded on mobile genetic elements, facilitating their rapid spread amongst Gram-positive bacteria, and are thought to confer resistance by binding to the ribosome and dislodging the bound antibiotic. However, the mechanistic basis of this resistance remains unclear. Here we refine the PoxtA spectrum of action, demonstrate alleviation of linezolid-induced context-dependent translational stalling, and present cryo-electron microscopy structures of PoxtA in complex with the Enterococcus faecalis 70S ribosome. PoxtA perturbs the CCA-end of the P-site tRNA, causing it to shift by ∼4 Å out of the ribosome, corresponding to a register shift of approximately one amino acid for an attached nascent polypeptide chain. We postulate that the perturbation of the P-site tRNA by PoxtA thereby alters the conformation of the attached nascent chain to disrupt the drug binding site.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; ; and , et al. (More)
; ; ; ; ; ; ; ; ; ; ; and (Less)
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Anti-Bacterial Agents/pharmacology, Cryoelectron Microscopy, Drug Resistance, Bacterial/genetics, Enterococcus faecalis/genetics, Linezolid/pharmacology, Oxazolidinones/pharmacology, RNA, Transfer/genetics
in
Nature Communications
volume
13
article number
1860
publisher
Nature Publishing Group
external identifiers
  • pmid:35387982
  • scopus:85127639432
ISSN
2041-1723
DOI
10.1038/s41467-022-29274-9
language
English
LU publication?
yes
additional info
© 2022. The Author(s).
id
27bb876f-5f6e-4264-8c21-d5e8d15af37b
date added to LUP
2022-04-12 07:29:33
date last changed
2024-06-28 01:38:25
@article{27bb876f-5f6e-4264-8c21-d5e8d15af37b,
  abstract     = {{<p>PoxtA and OptrA are ATP binding cassette (ABC) proteins of the F subtype (ABCF). They confer resistance to oxazolidinone and phenicol antibiotics, such as linezolid and chloramphenicol, which stall translating ribosomes when certain amino acids are present at a defined position in the nascent polypeptide chain. These proteins are often encoded on mobile genetic elements, facilitating their rapid spread amongst Gram-positive bacteria, and are thought to confer resistance by binding to the ribosome and dislodging the bound antibiotic. However, the mechanistic basis of this resistance remains unclear. Here we refine the PoxtA spectrum of action, demonstrate alleviation of linezolid-induced context-dependent translational stalling, and present cryo-electron microscopy structures of PoxtA in complex with the Enterococcus faecalis 70S ribosome. PoxtA perturbs the CCA-end of the P-site tRNA, causing it to shift by ∼4 Å out of the ribosome, corresponding to a register shift of approximately one amino acid for an attached nascent polypeptide chain. We postulate that the perturbation of the P-site tRNA by PoxtA thereby alters the conformation of the attached nascent chain to disrupt the drug binding site.</p>}},
  author       = {{Crowe-McAuliffe, Caillan and Murina, Victoriia and Turnbull, Kathryn Jane and Huch, Susanne and Kasari, Marje and Takada, Hiraku and Nersisyan, Lilit and Sundsfjord, Arnfinn and Hegstad, Kristin and Atkinson, Gemma C and Pelechano, Vicent and Wilson, Daniel N and Hauryliuk, Vasili}},
  issn         = {{2041-1723}},
  keywords     = {{Anti-Bacterial Agents/pharmacology; Cryoelectron Microscopy; Drug Resistance, Bacterial/genetics; Enterococcus faecalis/genetics; Linezolid/pharmacology; Oxazolidinones/pharmacology; RNA, Transfer/genetics}},
  language     = {{eng}},
  publisher    = {{Nature Publishing Group}},
  series       = {{Nature Communications}},
  title        = {{Structural basis for PoxtA-mediated resistance to phenicol and oxazolidinone antibiotics}},
  url          = {{http://dx.doi.org/10.1038/s41467-022-29274-9}},
  doi          = {{10.1038/s41467-022-29274-9}},
  volume       = {{13}},
  year         = {{2022}},
}