Structural basis for PoxtA-mediated resistance to phenicol and oxazolidinone antibiotics
(2022) In Nature Communications 13.- Abstract
PoxtA and OptrA are ATP binding cassette (ABC) proteins of the F subtype (ABCF). They confer resistance to oxazolidinone and phenicol antibiotics, such as linezolid and chloramphenicol, which stall translating ribosomes when certain amino acids are present at a defined position in the nascent polypeptide chain. These proteins are often encoded on mobile genetic elements, facilitating their rapid spread amongst Gram-positive bacteria, and are thought to confer resistance by binding to the ribosome and dislodging the bound antibiotic. However, the mechanistic basis of this resistance remains unclear. Here we refine the PoxtA spectrum of action, demonstrate alleviation of linezolid-induced context-dependent translational stalling, and... (More)
PoxtA and OptrA are ATP binding cassette (ABC) proteins of the F subtype (ABCF). They confer resistance to oxazolidinone and phenicol antibiotics, such as linezolid and chloramphenicol, which stall translating ribosomes when certain amino acids are present at a defined position in the nascent polypeptide chain. These proteins are often encoded on mobile genetic elements, facilitating their rapid spread amongst Gram-positive bacteria, and are thought to confer resistance by binding to the ribosome and dislodging the bound antibiotic. However, the mechanistic basis of this resistance remains unclear. Here we refine the PoxtA spectrum of action, demonstrate alleviation of linezolid-induced context-dependent translational stalling, and present cryo-electron microscopy structures of PoxtA in complex with the Enterococcus faecalis 70S ribosome. PoxtA perturbs the CCA-end of the P-site tRNA, causing it to shift by ∼4 Å out of the ribosome, corresponding to a register shift of approximately one amino acid for an attached nascent polypeptide chain. We postulate that the perturbation of the P-site tRNA by PoxtA thereby alters the conformation of the attached nascent chain to disrupt the drug binding site.
(Less)
- author
- organization
- publishing date
- 2022
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Anti-Bacterial Agents/pharmacology, Cryoelectron Microscopy, Drug Resistance, Bacterial/genetics, Enterococcus faecalis/genetics, Linezolid/pharmacology, Oxazolidinones/pharmacology, RNA, Transfer/genetics
- in
- Nature Communications
- volume
- 13
- article number
- 1860
- publisher
- Nature Publishing Group
- external identifiers
-
- pmid:35387982
- scopus:85127639432
- ISSN
- 2041-1723
- DOI
- 10.1038/s41467-022-29274-9
- language
- English
- LU publication?
- yes
- additional info
- © 2022. The Author(s).
- id
- 27bb876f-5f6e-4264-8c21-d5e8d15af37b
- date added to LUP
- 2022-04-12 07:29:33
- date last changed
- 2024-06-12 21:37:28
@article{27bb876f-5f6e-4264-8c21-d5e8d15af37b, abstract = {{<p>PoxtA and OptrA are ATP binding cassette (ABC) proteins of the F subtype (ABCF). They confer resistance to oxazolidinone and phenicol antibiotics, such as linezolid and chloramphenicol, which stall translating ribosomes when certain amino acids are present at a defined position in the nascent polypeptide chain. These proteins are often encoded on mobile genetic elements, facilitating their rapid spread amongst Gram-positive bacteria, and are thought to confer resistance by binding to the ribosome and dislodging the bound antibiotic. However, the mechanistic basis of this resistance remains unclear. Here we refine the PoxtA spectrum of action, demonstrate alleviation of linezolid-induced context-dependent translational stalling, and present cryo-electron microscopy structures of PoxtA in complex with the Enterococcus faecalis 70S ribosome. PoxtA perturbs the CCA-end of the P-site tRNA, causing it to shift by ∼4 Å out of the ribosome, corresponding to a register shift of approximately one amino acid for an attached nascent polypeptide chain. We postulate that the perturbation of the P-site tRNA by PoxtA thereby alters the conformation of the attached nascent chain to disrupt the drug binding site.</p>}}, author = {{Crowe-McAuliffe, Caillan and Murina, Victoriia and Turnbull, Kathryn Jane and Huch, Susanne and Kasari, Marje and Takada, Hiraku and Nersisyan, Lilit and Sundsfjord, Arnfinn and Hegstad, Kristin and Atkinson, Gemma C and Pelechano, Vicent and Wilson, Daniel N and Hauryliuk, Vasili}}, issn = {{2041-1723}}, keywords = {{Anti-Bacterial Agents/pharmacology; Cryoelectron Microscopy; Drug Resistance, Bacterial/genetics; Enterococcus faecalis/genetics; Linezolid/pharmacology; Oxazolidinones/pharmacology; RNA, Transfer/genetics}}, language = {{eng}}, publisher = {{Nature Publishing Group}}, series = {{Nature Communications}}, title = {{Structural basis for PoxtA-mediated resistance to phenicol and oxazolidinone antibiotics}}, url = {{http://dx.doi.org/10.1038/s41467-022-29274-9}}, doi = {{10.1038/s41467-022-29274-9}}, volume = {{13}}, year = {{2022}}, }