Different Requirements for Early and Late Phases of Azurophilic Granule-Phagosome Fusion.
Nordenfelt, Pontus LU
; Winberg, Martin E
; Lönnbro, Per
LU
; Rasmusson, Birgitta
and Tapper, Hans
LU
(2009)
In Traffic: the International Journal of Intracellular Transport
10.
p.1881-1893
- Abstract
- Abstract Phagocytosis and killing of microorganisms are complex processes that involve tightly regulated membrane traffic events. Because many signaling molecules associate with membrane rafts and because these structures can be found on azurophilic granules, we decided to investigate raft recruitment and the signaling requirements for azurophilic granule secretion during phagosome maturation. At the site of phagocytosis of immunoglobulin G-opsonized prey in human neutrophils, we found that early secretion of azurophilic granules was both raft- and calcium-dependent. Subsequently, rafts at the phagocytic site were internalized with the prey. At the fully formed phagosome, the fusion of azurophilic granules was no longer dependent on rafts... (More)
- Abstract Phagocytosis and killing of microorganisms are complex processes that involve tightly regulated membrane traffic events. Because many signaling molecules associate with membrane rafts and because these structures can be found on azurophilic granules, we decided to investigate raft recruitment and the signaling requirements for azurophilic granule secretion during phagosome maturation. At the site of phagocytosis of immunoglobulin G-opsonized prey in human neutrophils, we found that early secretion of azurophilic granules was both raft- and calcium-dependent. Subsequently, rafts at the phagocytic site were internalized with the prey. At the fully formed phagosome, the fusion of azurophilic granules was no longer dependent on rafts or calcium. These findings were found to be true also when using Streptococcus pyogenes bacteria as prey, and depletion of calcium affected the kinetics of bacterial intracellular survival. These findings suggest that the mechanisms for delivery of azurophilic content to nascent and sealed phagosomes, respectively, differ in their dependence on calcium and membrane rafts. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1500664
- author
- Nordenfelt, Pontus
LU
; Winberg, Martin E
; Lönnbro, Per
LU
; Rasmusson, Birgitta
and Tapper, Hans
LU
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Traffic: the International Journal of Intracellular Transport
- volume
- 10
- pages
- 1881 - 1893
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000271714300014
- pmid:19804565
- scopus:70649099317
- pmid:19804565
- ISSN
- 1398-9219
- DOI
- 10.1111/j.1600-0854.2009.00986.x
- language
- English
- LU publication?
- yes
- id
- 27dd3dec-8a5a-4a30-be40-8cac7344c8cf (old id 1500664)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/19804565?dopt=Abstract
- date added to LUP
- 2016-04-04 07:17:31
- date last changed
- 2023-03-20 19:00:36
@article{27dd3dec-8a5a-4a30-be40-8cac7344c8cf,
abstract = {{Abstract Phagocytosis and killing of microorganisms are complex processes that involve tightly regulated membrane traffic events. Because many signaling molecules associate with membrane rafts and because these structures can be found on azurophilic granules, we decided to investigate raft recruitment and the signaling requirements for azurophilic granule secretion during phagosome maturation. At the site of phagocytosis of immunoglobulin G-opsonized prey in human neutrophils, we found that early secretion of azurophilic granules was both raft- and calcium-dependent. Subsequently, rafts at the phagocytic site were internalized with the prey. At the fully formed phagosome, the fusion of azurophilic granules was no longer dependent on rafts or calcium. These findings were found to be true also when using Streptococcus pyogenes bacteria as prey, and depletion of calcium affected the kinetics of bacterial intracellular survival. These findings suggest that the mechanisms for delivery of azurophilic content to nascent and sealed phagosomes, respectively, differ in their dependence on calcium and membrane rafts.}},
author = {{Nordenfelt, Pontus and Winberg, Martin E and Lönnbro, Per and Rasmusson, Birgitta and Tapper, Hans}},
issn = {{1398-9219}},
language = {{eng}},
pages = {{1881--1893}},
publisher = {{Wiley-Blackwell}},
series = {{Traffic: the International Journal of Intracellular Transport}},
title = {{Different Requirements for Early and Late Phases of Azurophilic Granule-Phagosome Fusion.}},
url = {{http://dx.doi.org/10.1111/j.1600-0854.2009.00986.x}},
doi = {{10.1111/j.1600-0854.2009.00986.x}},
volume = {{10}},
year = {{2009}},
}