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HPV-16 L1 genes with inactivated negative RNA elements induce potent immune responses

Rollman, E; Arnheim, L; Collier, B; Oberg, D; Hall, H; Klingstrom, J; Dillner, Joakim LU ; Pastrana, DV; Buck, CB and Hinkula, J, et al. (2004) In Virology 322(1). p.182-189
Abstract
Introduction of point mutations in the 5' end of the human papillomavirus type 16 (HPV-16) L1 gene specifically inactivates negative regulatory RNA processing elements. DNA vaccination of C57B1/6 mice with the mutated L1 gene resulted in improved immunogenicity for both neutralizing antibodies as well as for broad cellular immune responses. Previous reports on the activation of L1 by codon optimization may be explained by inactivation of the regulatory RNA elements. The modified HPV-16 L1 DNA that induced anti-HPV-16 immunity may be seen as a complementary approach to protein subunit immunization against papillomavirus.
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
imiquimod, rGM-CSF, T lymphocytes, neutralization, tumor immunity, DNA vaccination, HPV-16
in
Virology
volume
322
issue
1
pages
182 - 189
publisher
Elsevier
external identifiers
  • pmid:15063127
  • wos:000220861100017
  • scopus:11144354313
ISSN
1096-0341
DOI
10.1016/j.virol.2004.02.002
language
English
LU publication?
yes
id
e845d1a2-0c55-4123-a9bf-2eb197e08b75 (old id 280565)
date added to LUP
2007-10-26 17:07:54
date last changed
2017-01-01 07:01:04
@article{e845d1a2-0c55-4123-a9bf-2eb197e08b75,
  abstract     = {Introduction of point mutations in the 5' end of the human papillomavirus type 16 (HPV-16) L1 gene specifically inactivates negative regulatory RNA processing elements. DNA vaccination of C57B1/6 mice with the mutated L1 gene resulted in improved immunogenicity for both neutralizing antibodies as well as for broad cellular immune responses. Previous reports on the activation of L1 by codon optimization may be explained by inactivation of the regulatory RNA elements. The modified HPV-16 L1 DNA that induced anti-HPV-16 immunity may be seen as a complementary approach to protein subunit immunization against papillomavirus.},
  author       = {Rollman, E and Arnheim, L and Collier, B and Oberg, D and Hall, H and Klingstrom, J and Dillner, Joakim and Pastrana, DV and Buck, CB and Hinkula, J and Wahren, B and Schwartz, S},
  issn         = {1096-0341},
  keyword      = {imiquimod,rGM-CSF,T lymphocytes,neutralization,tumor immunity,DNA vaccination,HPV-16},
  language     = {eng},
  number       = {1},
  pages        = {182--189},
  publisher    = {Elsevier},
  series       = {Virology},
  title        = {HPV-16 L1 genes with inactivated negative RNA elements induce potent immune responses},
  url          = {http://dx.doi.org/10.1016/j.virol.2004.02.002},
  volume       = {322},
  year         = {2004},
}