HPV-16 L1 genes with inactivated negative RNA elements induce potent immune responses
(2004) In Virology 322(1). p.182-189- Abstract
- Introduction of point mutations in the 5' end of the human papillomavirus type 16 (HPV-16) L1 gene specifically inactivates negative regulatory RNA processing elements. DNA vaccination of C57B1/6 mice with the mutated L1 gene resulted in improved immunogenicity for both neutralizing antibodies as well as for broad cellular immune responses. Previous reports on the activation of L1 by codon optimization may be explained by inactivation of the regulatory RNA elements. The modified HPV-16 L1 DNA that induced anti-HPV-16 immunity may be seen as a complementary approach to protein subunit immunization against papillomavirus.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/280565
- author
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- imiquimod, rGM-CSF, T lymphocytes, neutralization, tumor immunity, DNA vaccination, HPV-16
- in
- Virology
- volume
- 322
- issue
- 1
- pages
- 182 - 189
- publisher
- Elsevier
- external identifiers
-
- pmid:15063127
- wos:000220861100017
- scopus:11144354313
- pmid:15063127
- ISSN
- 1096-0341
- DOI
- 10.1016/j.virol.2004.02.002
- language
- English
- LU publication?
- yes
- id
- e845d1a2-0c55-4123-a9bf-2eb197e08b75 (old id 280565)
- date added to LUP
- 2016-04-01 16:17:12
- date last changed
- 2022-03-14 23:24:33
@article{e845d1a2-0c55-4123-a9bf-2eb197e08b75, abstract = {{Introduction of point mutations in the 5' end of the human papillomavirus type 16 (HPV-16) L1 gene specifically inactivates negative regulatory RNA processing elements. DNA vaccination of C57B1/6 mice with the mutated L1 gene resulted in improved immunogenicity for both neutralizing antibodies as well as for broad cellular immune responses. Previous reports on the activation of L1 by codon optimization may be explained by inactivation of the regulatory RNA elements. The modified HPV-16 L1 DNA that induced anti-HPV-16 immunity may be seen as a complementary approach to protein subunit immunization against papillomavirus.}}, author = {{Rollman, E and Arnheim, L and Collier, B and Oberg, D and Hall, H and Klingstrom, J and Dillner, Joakim and Pastrana, DV and Buck, CB and Hinkula, J and Wahren, B and Schwartz, S}}, issn = {{1096-0341}}, keywords = {{imiquimod; rGM-CSF; T lymphocytes; neutralization; tumor immunity; DNA vaccination; HPV-16}}, language = {{eng}}, number = {{1}}, pages = {{182--189}}, publisher = {{Elsevier}}, series = {{Virology}}, title = {{HPV-16 L1 genes with inactivated negative RNA elements induce potent immune responses}}, url = {{http://dx.doi.org/10.1016/j.virol.2004.02.002}}, doi = {{10.1016/j.virol.2004.02.002}}, volume = {{322}}, year = {{2004}}, }