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Controlled-pH Tissue Cleanup Protocol for Signal Enhancement of Small Molecule Drugs Analyzed by MALDI-MS Imaging

Shariatgorji, Mohammadreza; Kallback, Patrik; Gustavsson, Lena LU ; Schintu, Nicoletta; Svenningsson, Per; Goodwin, Richard J. A. and Andren, Per E. (2012) In Analytical Chemistry 84(10). p.4603-4607
Abstract
The limit of detection of low-molecular weight compounds in tissue sections, analyzed by matrix assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI), was significantly improved by employing sample washing using a pH-controlled buffer solution. The pH of the washing solutions were set at values whereby the target analytes would have low solubility. Washing the tissue sections in the buffered solution resulted in removal of endogenous soluble ionization-suppressing compounds and salts, while the target compound remained in situ with minor or no delocalization during the buffered washing procedure. Two pharmaceutical compounds (cimetidine and imipramine) and one new protease inhibitor compound were successfully used to... (More)
The limit of detection of low-molecular weight compounds in tissue sections, analyzed by matrix assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI), was significantly improved by employing sample washing using a pH-controlled buffer solution. The pH of the washing solutions were set at values whereby the target analytes would have low solubility. Washing the tissue sections in the buffered solution resulted in removal of endogenous soluble ionization-suppressing compounds and salts, while the target compound remained in situ with minor or no delocalization during the buffered washing procedure. Two pharmaceutical compounds (cimetidine and imipramine) and one new protease inhibitor compound were successfully used to evaluate the feasibility of the pH-controlled tissue washing protocol for MALDI-MSI. Enhancement in signal-to-noise ratio was achieved by a factor of up to 10. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical Chemistry
volume
84
issue
10
pages
4603 - 4607
publisher
The American Chemical Society
external identifiers
  • wos:000303965500050
  • scopus:84861168071
ISSN
1520-6882
DOI
10.1021/ac203322q
language
English
LU publication?
yes
id
137c38e8-7f49-457b-a98d-25c18678ca96 (old id 2812957)
date added to LUP
2012-07-03 10:24:12
date last changed
2017-07-23 03:10:12
@article{137c38e8-7f49-457b-a98d-25c18678ca96,
  abstract     = {The limit of detection of low-molecular weight compounds in tissue sections, analyzed by matrix assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI), was significantly improved by employing sample washing using a pH-controlled buffer solution. The pH of the washing solutions were set at values whereby the target analytes would have low solubility. Washing the tissue sections in the buffered solution resulted in removal of endogenous soluble ionization-suppressing compounds and salts, while the target compound remained in situ with minor or no delocalization during the buffered washing procedure. Two pharmaceutical compounds (cimetidine and imipramine) and one new protease inhibitor compound were successfully used to evaluate the feasibility of the pH-controlled tissue washing protocol for MALDI-MSI. Enhancement in signal-to-noise ratio was achieved by a factor of up to 10.},
  author       = {Shariatgorji, Mohammadreza and Kallback, Patrik and Gustavsson, Lena and Schintu, Nicoletta and Svenningsson, Per and Goodwin, Richard J. A. and Andren, Per E.},
  issn         = {1520-6882},
  language     = {eng},
  number       = {10},
  pages        = {4603--4607},
  publisher    = {The American Chemical Society},
  series       = {Analytical Chemistry},
  title        = {Controlled-pH Tissue Cleanup Protocol for Signal Enhancement of Small Molecule Drugs Analyzed by MALDI-MS Imaging},
  url          = {http://dx.doi.org/10.1021/ac203322q},
  volume       = {84},
  year         = {2012},
}