Controlled-pH Tissue Cleanup Protocol for Signal Enhancement of Small Molecule Drugs Analyzed by MALDI-MS Imaging
(2012) In Analytical Chemistry 84(10). p.4603-4607- Abstract
- The limit of detection of low-molecular weight compounds in tissue sections, analyzed by matrix assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI), was significantly improved by employing sample washing using a pH-controlled buffer solution. The pH of the washing solutions were set at values whereby the target analytes would have low solubility. Washing the tissue sections in the buffered solution resulted in removal of endogenous soluble ionization-suppressing compounds and salts, while the target compound remained in situ with minor or no delocalization during the buffered washing procedure. Two pharmaceutical compounds (cimetidine and imipramine) and one new protease inhibitor compound were successfully used to... (More)
- The limit of detection of low-molecular weight compounds in tissue sections, analyzed by matrix assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI), was significantly improved by employing sample washing using a pH-controlled buffer solution. The pH of the washing solutions were set at values whereby the target analytes would have low solubility. Washing the tissue sections in the buffered solution resulted in removal of endogenous soluble ionization-suppressing compounds and salts, while the target compound remained in situ with minor or no delocalization during the buffered washing procedure. Two pharmaceutical compounds (cimetidine and imipramine) and one new protease inhibitor compound were successfully used to evaluate the feasibility of the pH-controlled tissue washing protocol for MALDI-MSI. Enhancement in signal-to-noise ratio was achieved by a factor of up to 10. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2812957
- author
- Shariatgorji, Mohammadreza ; Kallback, Patrik ; Gustavsson, Lena LU ; Schintu, Nicoletta ; Svenningsson, Per ; Goodwin, Richard J. A. and Andren, Per E.
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Analytical Chemistry
- volume
- 84
- issue
- 10
- pages
- 4603 - 4607
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000303965500050
- scopus:84861168071
- pmid:22507246
- ISSN
- 1520-6882
- DOI
- 10.1021/ac203322q
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Tumour Cell Biology (013017530)
- id
- 137c38e8-7f49-457b-a98d-25c18678ca96 (old id 2812957)
- date added to LUP
- 2016-04-01 10:12:22
- date last changed
- 2022-04-19 23:46:39
@article{137c38e8-7f49-457b-a98d-25c18678ca96, abstract = {{The limit of detection of low-molecular weight compounds in tissue sections, analyzed by matrix assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI), was significantly improved by employing sample washing using a pH-controlled buffer solution. The pH of the washing solutions were set at values whereby the target analytes would have low solubility. Washing the tissue sections in the buffered solution resulted in removal of endogenous soluble ionization-suppressing compounds and salts, while the target compound remained in situ with minor or no delocalization during the buffered washing procedure. Two pharmaceutical compounds (cimetidine and imipramine) and one new protease inhibitor compound were successfully used to evaluate the feasibility of the pH-controlled tissue washing protocol for MALDI-MSI. Enhancement in signal-to-noise ratio was achieved by a factor of up to 10.}}, author = {{Shariatgorji, Mohammadreza and Kallback, Patrik and Gustavsson, Lena and Schintu, Nicoletta and Svenningsson, Per and Goodwin, Richard J. A. and Andren, Per E.}}, issn = {{1520-6882}}, language = {{eng}}, number = {{10}}, pages = {{4603--4607}}, publisher = {{The American Chemical Society (ACS)}}, series = {{Analytical Chemistry}}, title = {{Controlled-pH Tissue Cleanup Protocol for Signal Enhancement of Small Molecule Drugs Analyzed by MALDI-MS Imaging}}, url = {{http://dx.doi.org/10.1021/ac203322q}}, doi = {{10.1021/ac203322q}}, volume = {{84}}, year = {{2012}}, }