Role of angiotensin II in ischemia/reperfusion-induced leukocyte-endothelium interactions in the colon
(2004) In FASEB Journal 18(3). p.881-881- Abstract
- The aims of the present study were to determine the effects and mechanisms of angiotensin II (Ang II) on leukocyte-endothelium interactions and the role of Ang II in a novel model of ischemia/reperfusion (I/R) in the mouse colon. Ang II dose-dependently increased leukocyte rolling and adhesion in colonic venules. Importantly, Ang II-induced leukocyte rolling was completely inhibited by immunoneutralization of P-selectin, and leukocyte adhesion was abolished in lymphocyte function antigen-1 (LFA-1)-deficient mice. The P-selectin-dependent rolling was found to be a precondition for the subsequent LFA-1-dependent leukocyte adhesion. Moreover, Ang II-induced leukocyte responses involved generation of reactive oxygen species and up-regulation... (More)
- The aims of the present study were to determine the effects and mechanisms of angiotensin II (Ang II) on leukocyte-endothelium interactions and the role of Ang II in a novel model of ischemia/reperfusion (I/R) in the mouse colon. Ang II dose-dependently increased leukocyte rolling and adhesion in colonic venules. Importantly, Ang II-induced leukocyte rolling was completely inhibited by immunoneutralization of P-selectin, and leukocyte adhesion was abolished in lymphocyte function antigen-1 (LFA-1)-deficient mice. The P-selectin-dependent rolling was found to be a precondition for the subsequent LFA-1-dependent leukocyte adhesion. Moreover, Ang II-induced leukocyte responses involved generation of reactive oxygen species and up-regulation of CXC chemokines. Notably, CXC chemokines, but not Ang II, stimulated leukocyte chemotaxis in vitro. I/R increased gene expression of angiotensin converting enzyme (ACE) in the colon and plasma concentrations of Ang II. Inhibition of ACE and the type 1 angiotensin (AT(1)) receptor significantly decreased the I/R-induced leukocyte adhesion. Taken together, these novel findings demonstrate that Ang II exerts potent pro-inflammatory effects in the colonic microcirculation and that inhibition of Ang II expression or function protects against I/R-induced leukocyte responses in the colon. Thus, it is suggested that Ang II is a major target to control pathological inflammation in the colon. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/283680
- author
- Riaz, AA ; Wang, Yusheng LU ; Schramm, R ; Sato, T ; Menger, MD ; Jeppsson, Bengt LU and Thorlacius, Henrik LU
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- adhesion, inflammation, microcirculation, P-selectin
- in
- FASEB Journal
- volume
- 18
- issue
- 3
- pages
- 881 - 881
- publisher
- Wiley
- external identifiers
-
- wos:000220522800026
- scopus:4644363433
- ISSN
- 1530-6860
- DOI
- 10.1096/fj.03-0502fje
- language
- English
- LU publication?
- yes
- id
- df7d981f-fd8f-46dd-9278-84dd7bd85016 (old id 283680)
- date added to LUP
- 2016-04-01 15:36:34
- date last changed
- 2023-09-04 04:11:18
@article{df7d981f-fd8f-46dd-9278-84dd7bd85016, abstract = {{The aims of the present study were to determine the effects and mechanisms of angiotensin II (Ang II) on leukocyte-endothelium interactions and the role of Ang II in a novel model of ischemia/reperfusion (I/R) in the mouse colon. Ang II dose-dependently increased leukocyte rolling and adhesion in colonic venules. Importantly, Ang II-induced leukocyte rolling was completely inhibited by immunoneutralization of P-selectin, and leukocyte adhesion was abolished in lymphocyte function antigen-1 (LFA-1)-deficient mice. The P-selectin-dependent rolling was found to be a precondition for the subsequent LFA-1-dependent leukocyte adhesion. Moreover, Ang II-induced leukocyte responses involved generation of reactive oxygen species and up-regulation of CXC chemokines. Notably, CXC chemokines, but not Ang II, stimulated leukocyte chemotaxis in vitro. I/R increased gene expression of angiotensin converting enzyme (ACE) in the colon and plasma concentrations of Ang II. Inhibition of ACE and the type 1 angiotensin (AT(1)) receptor significantly decreased the I/R-induced leukocyte adhesion. Taken together, these novel findings demonstrate that Ang II exerts potent pro-inflammatory effects in the colonic microcirculation and that inhibition of Ang II expression or function protects against I/R-induced leukocyte responses in the colon. Thus, it is suggested that Ang II is a major target to control pathological inflammation in the colon.}}, author = {{Riaz, AA and Wang, Yusheng and Schramm, R and Sato, T and Menger, MD and Jeppsson, Bengt and Thorlacius, Henrik}}, issn = {{1530-6860}}, keywords = {{adhesion; inflammation; microcirculation; P-selectin}}, language = {{eng}}, number = {{3}}, pages = {{881--881}}, publisher = {{Wiley}}, series = {{FASEB Journal}}, title = {{Role of angiotensin II in ischemia/reperfusion-induced leukocyte-endothelium interactions in the colon}}, url = {{http://dx.doi.org/10.1096/fj.03-0502fje}}, doi = {{10.1096/fj.03-0502fje}}, volume = {{18}}, year = {{2004}}, }