Advanced

Separation of soluble selenium compounds in muscle from seven animal species using size exclusion chromatography and inductively coupled plasma mass spectrometry

Daun, Charlotte LU ; Lundh, Thomas LU ; Önning, Gunilla LU and Åkesson, Björn LU (2004) In Journal of Analytical Atomic Spectrometry 19(1). p.129-134
Abstract
To study the role of selenium compounds for the quality and nutritional value of meat, speciation of selenium compounds was performed in two muscles from each of seven animal species (chicken, turkey, duck, ostrich, lamb, cattle and pig). Soluble selenium compounds were separated by size-exclusion chromatography (SEC) and detected by inductively coupled plasma-mass spectrometry (ICP-MS). Four selenium peaks were found in the muscle extracts by SEC, with partition coefficients (K-av) of 0.02, 0.44, 0.74 and 0.86, respectively. The second and third peaks had a chromatographic mobility approximately corresponding to that of tetrameric glutathione peroxidase and selenoprotein W, respectively, and they contained 68-100% of the recovered... (More)
To study the role of selenium compounds for the quality and nutritional value of meat, speciation of selenium compounds was performed in two muscles from each of seven animal species (chicken, turkey, duck, ostrich, lamb, cattle and pig). Soluble selenium compounds were separated by size-exclusion chromatography (SEC) and detected by inductively coupled plasma-mass spectrometry (ICP-MS). Four selenium peaks were found in the muscle extracts by SEC, with partition coefficients (K-av) of 0.02, 0.44, 0.74 and 0.86, respectively. The second and third peaks had a chromatographic mobility approximately corresponding to that of tetrameric glutathione peroxidase and selenoprotein W, respectively, and they contained 68-100% of the recovered selenium in different muscles. The distribution of selenium among the four peaks varied considerably in muscles from different species, the second peak accounting for 28-71% of the recovered selenium and the third peak for 17-72%. These differences in selenium distribution among animal species are discussed in relation to meat quality and nutritional value. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Analytical Atomic Spectrometry
volume
19
issue
1
pages
129 - 134
publisher
Royal Society of Chemistry
external identifiers
  • wos:000220253500020
  • scopus:1342327351
ISSN
1364-5544
DOI
10.1039/b306916h
language
English
LU publication?
yes
id
6837e99d-986e-423e-b619-65f36c793f2e (old id 284859)
date added to LUP
2007-10-23 09:51:20
date last changed
2017-06-18 03:50:31
@article{6837e99d-986e-423e-b619-65f36c793f2e,
  abstract     = {To study the role of selenium compounds for the quality and nutritional value of meat, speciation of selenium compounds was performed in two muscles from each of seven animal species (chicken, turkey, duck, ostrich, lamb, cattle and pig). Soluble selenium compounds were separated by size-exclusion chromatography (SEC) and detected by inductively coupled plasma-mass spectrometry (ICP-MS). Four selenium peaks were found in the muscle extracts by SEC, with partition coefficients (K-av) of 0.02, 0.44, 0.74 and 0.86, respectively. The second and third peaks had a chromatographic mobility approximately corresponding to that of tetrameric glutathione peroxidase and selenoprotein W, respectively, and they contained 68-100% of the recovered selenium in different muscles. The distribution of selenium among the four peaks varied considerably in muscles from different species, the second peak accounting for 28-71% of the recovered selenium and the third peak for 17-72%. These differences in selenium distribution among animal species are discussed in relation to meat quality and nutritional value.},
  author       = {Daun, Charlotte and Lundh, Thomas and Önning, Gunilla and Åkesson, Björn},
  issn         = {1364-5544},
  language     = {eng},
  number       = {1},
  pages        = {129--134},
  publisher    = {Royal Society of Chemistry},
  series       = {Journal of Analytical Atomic Spectrometry},
  title        = {Separation of soluble selenium compounds in muscle from seven animal species using size exclusion chromatography and inductively coupled plasma mass spectrometry},
  url          = {http://dx.doi.org/10.1039/b306916h},
  volume       = {19},
  year         = {2004},
}