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Design of recombinant antibody microarrays for urinary proteomics.

Kristensson, Malin; Olsson, Karolina; Carlson, Joyce LU ; Wullt, Björn LU ; Sturfelt, Gunnar LU ; Borrebaeck, Carl A K and Wingren, Christer (2012) In Proteomics Clinical Applications 6(5-6). p.291-296
Abstract
PURPOSE:

Urinary proteomics has become a key discipline within clinical proteomics for noninvasive diagnosis and monitoring of disease, and biomarker discovery. In order to decipher complex proteomes, high demands will, however, be placed upon the methodology applied. The purpose of this study was to develop a recombinant antibody microarray platform for urinary proteomics.



EXPERIMENTAL DESIGN:

We adopted our previously in-house developed recombinant antibody microarray set-up and redesigned the platform for urinary proteomics. In this process, the key antibody array assay parameters, such as sample handling, sample labeling protocol, and assay conditions, etc, reflecting the unique properties of urine... (More)
PURPOSE:

Urinary proteomics has become a key discipline within clinical proteomics for noninvasive diagnosis and monitoring of disease, and biomarker discovery. In order to decipher complex proteomes, high demands will, however, be placed upon the methodology applied. The purpose of this study was to develop a recombinant antibody microarray platform for urinary proteomics.



EXPERIMENTAL DESIGN:

We adopted our previously in-house developed recombinant antibody microarray set-up and redesigned the platform for urinary proteomics. In this process, the key antibody array assay parameters, such as sample handling, sample labeling protocol, and assay conditions, etc, reflecting the unique properties of urine as sample format, were addressed and reoptimized in a step-by-step procedure.



RESULTS:

In this proof-of-concept study, we have designed the first generation of a recombinant antibody microarray technology platform for urinary proteomics. The results showed that multiplexed, sensitive (pg/mL range), and reproducible urine protein expression profiling could be performed targeting directly labeled, nonfractionated urine.



CONCLUSION AND CLINICAL RELEVANCE:

We have demonstrated that crude, directly labeled urine samples could be profiled in a rapid, reproducible, sensitive, and multiplexed manner after minimal sample prehandling. These findings could potentially pave the way for enhanced urinary proteomics and understanding of renal physiology with implications in both health and disease. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Proteomics Clinical Applications
volume
6
issue
5-6
pages
291 - 296
publisher
John Wiley & Sons
external identifiers
  • wos:000305823200006
  • pmid:22744810
  • scopus:84863227739
ISSN
1862-8354
DOI
10.1002/prca.201100055
project
CREATE Health
language
English
LU publication?
yes
id
af669e0d-4110-404f-beb6-dd5a8f0ebfb4 (old id 2858850)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/22744810?dopt=Abstract
date added to LUP
2012-07-04 20:44:41
date last changed
2017-04-09 04:28:17
@article{af669e0d-4110-404f-beb6-dd5a8f0ebfb4,
  abstract     = {PURPOSE:<br/><br>
Urinary proteomics has become a key discipline within clinical proteomics for noninvasive diagnosis and monitoring of disease, and biomarker discovery. In order to decipher complex proteomes, high demands will, however, be placed upon the methodology applied. The purpose of this study was to develop a recombinant antibody microarray platform for urinary proteomics.<br/><br>
<br/><br>
EXPERIMENTAL DESIGN:<br/><br>
We adopted our previously in-house developed recombinant antibody microarray set-up and redesigned the platform for urinary proteomics. In this process, the key antibody array assay parameters, such as sample handling, sample labeling protocol, and assay conditions, etc, reflecting the unique properties of urine as sample format, were addressed and reoptimized in a step-by-step procedure.<br/><br>
<br/><br>
RESULTS:<br/><br>
In this proof-of-concept study, we have designed the first generation of a recombinant antibody microarray technology platform for urinary proteomics. The results showed that multiplexed, sensitive (pg/mL range), and reproducible urine protein expression profiling could be performed targeting directly labeled, nonfractionated urine.<br/><br>
<br/><br>
CONCLUSION AND CLINICAL RELEVANCE:<br/><br>
We have demonstrated that crude, directly labeled urine samples could be profiled in a rapid, reproducible, sensitive, and multiplexed manner after minimal sample prehandling. These findings could potentially pave the way for enhanced urinary proteomics and understanding of renal physiology with implications in both health and disease.},
  author       = {Kristensson, Malin and Olsson, Karolina and Carlson, Joyce and Wullt, Björn and Sturfelt, Gunnar and Borrebaeck, Carl A K and Wingren, Christer},
  issn         = {1862-8354},
  language     = {eng},
  number       = {5-6},
  pages        = {291--296},
  publisher    = {John Wiley & Sons},
  series       = {Proteomics Clinical Applications},
  title        = {Design of recombinant antibody microarrays for urinary proteomics.},
  url          = {http://dx.doi.org/10.1002/prca.201100055},
  volume       = {6},
  year         = {2012},
}