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Determination of carotenoids in microalgae using supercritical fluid extraction and chromatography.

Abrahamsson, Victor LU ; Rodriguez Meizoso, Irene LU and Turner, Charlotta LU (2012) In Journal of chromatography. A 1250. p.63-68
Abstract
A method was developed based on supercritical fluid chromatography for quantitative determination of carotenoids in extracts of Scenedesmus sp. By utilizing the low backpressure in supercritical fluid chromatography, a C18 column and a 2-ethyl pyridine column were coupled in series. It was concluded that even minor changes in temperature had a substantial effect on selectivity. A standard mixture of 8 carotenoids and microalgae extracts obtained through supercritical fluid extraction with and without 10% ethanol as a co-solvent were successfully separated. All of the carotenoids were separated within 10min, while the total analysis time was 20min. The method was validated and the carotenoids of microalgae extracts were quantified.... (More)
A method was developed based on supercritical fluid chromatography for quantitative determination of carotenoids in extracts of Scenedesmus sp. By utilizing the low backpressure in supercritical fluid chromatography, a C18 column and a 2-ethyl pyridine column were coupled in series. It was concluded that even minor changes in temperature had a substantial effect on selectivity. A standard mixture of 8 carotenoids and microalgae extracts obtained through supercritical fluid extraction with and without 10% ethanol as a co-solvent were successfully separated. All of the carotenoids were separated within 10min, while the total analysis time was 20min. The method was validated and the carotenoids of microalgae extracts were quantified. Furthermore, the method should be seen as a more rapid and environmentally sustainable alternative to traditional high-performance liquid chromatography methods utilizing organic solvents. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of chromatography. A
volume
1250
pages
63 - 68
publisher
Elsevier
external identifiers
  • wos:000307089500007
  • pmid:22695695
  • scopus:84863864962
ISSN
1873-3778
DOI
10.1016/j.chroma.2012.05.069
language
English
LU publication?
yes
id
65105aba-d22d-4f51-af3b-df18f882e8d2 (old id 2859467)
date added to LUP
2012-07-06 15:41:00
date last changed
2017-07-02 04:07:13
@article{65105aba-d22d-4f51-af3b-df18f882e8d2,
  abstract     = {A method was developed based on supercritical fluid chromatography for quantitative determination of carotenoids in extracts of Scenedesmus sp. By utilizing the low backpressure in supercritical fluid chromatography, a C18 column and a 2-ethyl pyridine column were coupled in series. It was concluded that even minor changes in temperature had a substantial effect on selectivity. A standard mixture of 8 carotenoids and microalgae extracts obtained through supercritical fluid extraction with and without 10% ethanol as a co-solvent were successfully separated. All of the carotenoids were separated within 10min, while the total analysis time was 20min. The method was validated and the carotenoids of microalgae extracts were quantified. Furthermore, the method should be seen as a more rapid and environmentally sustainable alternative to traditional high-performance liquid chromatography methods utilizing organic solvents.},
  author       = {Abrahamsson, Victor and Rodriguez Meizoso, Irene and Turner, Charlotta},
  issn         = {1873-3778},
  language     = {eng},
  pages        = {63--68},
  publisher    = {Elsevier},
  series       = {Journal of chromatography. A},
  title        = {Determination of carotenoids in microalgae using supercritical fluid extraction and chromatography.},
  url          = {http://dx.doi.org/10.1016/j.chroma.2012.05.069},
  volume       = {1250},
  year         = {2012},
}