Advanced

Histamine and histidine decarboxylase are hallmark features of ECL cells but not G cells in rat stomach

Zhao, CM; Chen, D; de la Cour, Charlotta LU ; Lindqvist, A; Persson, L and Håkanson, Rolf LU (2004) In Regulatory Peptides 118(1-2). p.61-66
Abstract
The oxyntic mucosa of the rat stomach is rich in ECL cells which produce and secrete histamine in response to gastrin. Histamine and the histamine-forming enzyme histidine decarboxylase (HDC) have been claimed to occur also in the gastrin-secreting G cells in the antrum. In the present study, we used a panel of five HDC antisera and one histamine antiserum to investigate whether histamine and HDC are exclusive to the ECL cells. By immunocytochemistry, we could show that the ECL cells were stained with the histamine antiserum and all five HDC antisera. The G cells, however, were not stained with the histamine antiserum, but with three of the five HDC antisera. Thus, histamine and HDC coexist in the ECL cells (oxymic mucosa) but not in G... (More)
The oxyntic mucosa of the rat stomach is rich in ECL cells which produce and secrete histamine in response to gastrin. Histamine and the histamine-forming enzyme histidine decarboxylase (HDC) have been claimed to occur also in the gastrin-secreting G cells in the antrum. In the present study, we used a panel of five HDC antisera and one histamine antiserum to investigate whether histamine and HDC are exclusive to the ECL cells. By immunocytochemistry, we could show that the ECL cells were stained with the histamine antiserum and all five HDC antisera. The G cells, however, were not stained with the histamine antiserum, but with three of the five HDC antisera. Thus, histamine and HDC coexist in the ECL cells (oxymic mucosa) but not in G cells (antral mucosa). Western blot analysis revealed a typical pattern of HDC-immunoreactive bands (74, 63 and 54 kDa) in oxymic mucosa extracts with all five antisera. In antral extracts, immunoreactive bands were detected with three of the five HDC antisera (same as above); the pattern of immunoreactivity differed from that in oxymic mucosa. Food intake or treatment with the proton pump inhibitor omeprazole raised the HDC activity and the HDC protein content of the oxyntic mucosa but not of the antral mucosa; the HDC activity in the antrum was barely detectable. We suggest that the HDC-like immunoreactivity in the antrum represents a cross-reaction with non-HDC proteins and conclude that histamine and HDC are hallmark features of ECL cells but not of G cells. (C) 2003 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
histidine decarboxylase (HDC), histamine, ECL cells, G cells
in
Regulatory Peptides
volume
118
issue
1-2
pages
61 - 66
publisher
Elsevier
external identifiers
  • pmid:14759558
  • wos:000189088600009
  • scopus:0842303259
ISSN
1873-1686
DOI
10.1016/j.regpep.2003.10.031
language
English
LU publication?
yes
id
12d1cb2d-2e5d-4165-8491-b60e853a0fc5 (old id 286920)
date added to LUP
2007-11-07 10:18:58
date last changed
2017-01-01 05:01:08
@article{12d1cb2d-2e5d-4165-8491-b60e853a0fc5,
  abstract     = {The oxyntic mucosa of the rat stomach is rich in ECL cells which produce and secrete histamine in response to gastrin. Histamine and the histamine-forming enzyme histidine decarboxylase (HDC) have been claimed to occur also in the gastrin-secreting G cells in the antrum. In the present study, we used a panel of five HDC antisera and one histamine antiserum to investigate whether histamine and HDC are exclusive to the ECL cells. By immunocytochemistry, we could show that the ECL cells were stained with the histamine antiserum and all five HDC antisera. The G cells, however, were not stained with the histamine antiserum, but with three of the five HDC antisera. Thus, histamine and HDC coexist in the ECL cells (oxymic mucosa) but not in G cells (antral mucosa). Western blot analysis revealed a typical pattern of HDC-immunoreactive bands (74, 63 and 54 kDa) in oxymic mucosa extracts with all five antisera. In antral extracts, immunoreactive bands were detected with three of the five HDC antisera (same as above); the pattern of immunoreactivity differed from that in oxymic mucosa. Food intake or treatment with the proton pump inhibitor omeprazole raised the HDC activity and the HDC protein content of the oxyntic mucosa but not of the antral mucosa; the HDC activity in the antrum was barely detectable. We suggest that the HDC-like immunoreactivity in the antrum represents a cross-reaction with non-HDC proteins and conclude that histamine and HDC are hallmark features of ECL cells but not of G cells. (C) 2003 Elsevier B.V. All rights reserved.},
  author       = {Zhao, CM and Chen, D and de la Cour, Charlotta and Lindqvist, A and Persson, L and Håkanson, Rolf},
  issn         = {1873-1686},
  keyword      = {histidine decarboxylase (HDC),histamine,ECL cells,G cells},
  language     = {eng},
  number       = {1-2},
  pages        = {61--66},
  publisher    = {Elsevier},
  series       = {Regulatory Peptides},
  title        = {Histamine and histidine decarboxylase are hallmark features of ECL cells but not G cells in rat stomach},
  url          = {http://dx.doi.org/10.1016/j.regpep.2003.10.031},
  volume       = {118},
  year         = {2004},
}