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Heparin protects against skin flap necrosis: relationship to neutrophil recruitment and anti-coagulant activity

Torkvist, L; Lofberg, R; Raud, J and Thorlacius, Henrik LU (2004) In Inflammation Research1995-01-01+01:00 53(1). p.1-3
Abstract
Objective and design: Heparin has been shown to improve survival of surgical skin flaps. However, it is not known whether the protective effect of heparin is related to it's anticoagulative or anti-inflammatory effects. Methods: Surgical flaps were raised in the dorsal skin of Sprague-Dawley rats. Neutrophil recruitment was determined by measuring the tissue content of myeloperoxidase (MPO) and clotting time was estimated by assessment of activated partial thromboplastin time (APTT) in plasma. Results: Administration of heparin (150 U/kg) significantly increased skin flap survival from 44 % in vehicle-treated controls to 91 %. This heparin treatment increased APTT by 4.5 fold. However, administration of 150 U/kg of heparin had no effect on... (More)
Objective and design: Heparin has been shown to improve survival of surgical skin flaps. However, it is not known whether the protective effect of heparin is related to it's anticoagulative or anti-inflammatory effects. Methods: Surgical flaps were raised in the dorsal skin of Sprague-Dawley rats. Neutrophil recruitment was determined by measuring the tissue content of myeloperoxidase (MPO) and clotting time was estimated by assessment of activated partial thromboplastin time (APTT) in plasma. Results: Administration of heparin (150 U/kg) significantly increased skin flap survival from 44 % in vehicle-treated controls to 91 %. This heparin treatment increased APTT by 4.5 fold. However, administration of 150 U/kg of heparin had no effect on skin flap neutrophil recruitment. In contrast, we found that the polysaccharide fucoidan reduced MPO and also improved skin flap survival. Conclusions: In conclusion, we demonstrate that protective effect of clinically relevant doses of heparin correlates with its' ability to prolong clotting time and not to inhibition of neutrophil accumulation in the healing of skin flaps. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
leukocytes, activated partial thromboplastin time, heparin, neutrophils, surgical flaps
in
Inflammation Research1995-01-01+01:00
volume
53
issue
1
pages
1 - 3
publisher
Birkhaüser
external identifiers
  • wos:000188455500001
  • scopus:1642512307
ISSN
1420-908X
DOI
10.1007/s00011-003-1218-0
language
English
LU publication?
yes
id
e0d9ede0-cb55-4f16-9d7b-4ba7f0d53a5b (old id 289055)
date added to LUP
2007-10-26 09:18:04
date last changed
2017-01-01 04:34:01
@article{e0d9ede0-cb55-4f16-9d7b-4ba7f0d53a5b,
  abstract     = {Objective and design: Heparin has been shown to improve survival of surgical skin flaps. However, it is not known whether the protective effect of heparin is related to it's anticoagulative or anti-inflammatory effects. Methods: Surgical flaps were raised in the dorsal skin of Sprague-Dawley rats. Neutrophil recruitment was determined by measuring the tissue content of myeloperoxidase (MPO) and clotting time was estimated by assessment of activated partial thromboplastin time (APTT) in plasma. Results: Administration of heparin (150 U/kg) significantly increased skin flap survival from 44 % in vehicle-treated controls to 91 %. This heparin treatment increased APTT by 4.5 fold. However, administration of 150 U/kg of heparin had no effect on skin flap neutrophil recruitment. In contrast, we found that the polysaccharide fucoidan reduced MPO and also improved skin flap survival. Conclusions: In conclusion, we demonstrate that protective effect of clinically relevant doses of heparin correlates with its' ability to prolong clotting time and not to inhibition of neutrophil accumulation in the healing of skin flaps.},
  author       = {Torkvist, L and Lofberg, R and Raud, J and Thorlacius, Henrik},
  issn         = {1420-908X},
  keyword      = {leukocytes,activated partial thromboplastin time,heparin,neutrophils,surgical flaps},
  language     = {eng},
  number       = {1},
  pages        = {1--3},
  publisher    = {Birkhaüser},
  series       = {Inflammation Research1995-01-01+01:00},
  title        = {Heparin protects against skin flap necrosis: relationship to neutrophil recruitment and anti-coagulant activity},
  url          = {http://dx.doi.org/10.1007/s00011-003-1218-0},
  volume       = {53},
  year         = {2004},
}