The G alpha q/11 Proteins Contribute to T Lymphocyte Migration by Promoting Turnover of Integrin LFA-1 through Recycling
(2012) In PLoS ONE 7(6).- Abstract
- The role of G alpha i proteins coupled to chemokine receptors in directed migration of immune cells is well understood. In this study we show that the separate class of G alpha q/11 proteins is required for the underlying ability of T cells to migrate both randomly and in a directed chemokine-dependent manner. Interfering with G alpha q or G alpha 11 using dominant negative cDNA constructs or siRNA for G alpha q causes accumulation of LFA-1 adhesions and stalled migration. G alpha q/11 has an impact on LFA-1 expression at plasma membrane level and also on its internalization. Additionally G alpha q co-localizes with LFA-1- and EEA1-expressing intracellular vesicles and partially with Rap1- but not Rab11-expressing vesicles. However the... (More)
- The role of G alpha i proteins coupled to chemokine receptors in directed migration of immune cells is well understood. In this study we show that the separate class of G alpha q/11 proteins is required for the underlying ability of T cells to migrate both randomly and in a directed chemokine-dependent manner. Interfering with G alpha q or G alpha 11 using dominant negative cDNA constructs or siRNA for G alpha q causes accumulation of LFA-1 adhesions and stalled migration. G alpha q/11 has an impact on LFA-1 expression at plasma membrane level and also on its internalization. Additionally G alpha q co-localizes with LFA-1- and EEA1-expressing intracellular vesicles and partially with Rap1- but not Rab11-expressing vesicles. However the influence of G alpha q is not confined to the vesicles that express it, as its reduction alters intracellular trafficking of other vesicles involved in recycling. In summary vesicle-associated G alpha q/11 is required for the turnover of LFA-1 adhesion that is necessary for migration. These G proteins participate directly in the initial phase of recycling and this has an impact on later stages of the endo-exocytic pathway. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2891287
- author
- Svensson, Lena M LU ; Stanley, Paula ; Willenbrock, Frances and Hogg, Nancy
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- in
- PLoS ONE
- volume
- 7
- issue
- 6
- publisher
- Public Library of Science (PLoS)
- external identifiers
-
- wos:000305337600028
- scopus:84862206100
- pmid:22701657
- ISSN
- 1932-6203
- DOI
- 10.1371/journal.pone.0038517
- language
- English
- LU publication?
- yes
- id
- 980682f4-87a2-4b84-afe5-87d3c03325ca (old id 2891287)
- date added to LUP
- 2016-04-01 14:30:39
- date last changed
- 2022-01-28 00:57:28
@article{980682f4-87a2-4b84-afe5-87d3c03325ca, abstract = {{The role of G alpha i proteins coupled to chemokine receptors in directed migration of immune cells is well understood. In this study we show that the separate class of G alpha q/11 proteins is required for the underlying ability of T cells to migrate both randomly and in a directed chemokine-dependent manner. Interfering with G alpha q or G alpha 11 using dominant negative cDNA constructs or siRNA for G alpha q causes accumulation of LFA-1 adhesions and stalled migration. G alpha q/11 has an impact on LFA-1 expression at plasma membrane level and also on its internalization. Additionally G alpha q co-localizes with LFA-1- and EEA1-expressing intracellular vesicles and partially with Rap1- but not Rab11-expressing vesicles. However the influence of G alpha q is not confined to the vesicles that express it, as its reduction alters intracellular trafficking of other vesicles involved in recycling. In summary vesicle-associated G alpha q/11 is required for the turnover of LFA-1 adhesion that is necessary for migration. These G proteins participate directly in the initial phase of recycling and this has an impact on later stages of the endo-exocytic pathway.}}, author = {{Svensson, Lena M and Stanley, Paula and Willenbrock, Frances and Hogg, Nancy}}, issn = {{1932-6203}}, language = {{eng}}, number = {{6}}, publisher = {{Public Library of Science (PLoS)}}, series = {{PLoS ONE}}, title = {{The G alpha q/11 Proteins Contribute to T Lymphocyte Migration by Promoting Turnover of Integrin LFA-1 through Recycling}}, url = {{https://lup.lub.lu.se/search/files/4013147/3052969.pdf}}, doi = {{10.1371/journal.pone.0038517}}, volume = {{7}}, year = {{2012}}, }