Advanced

The G alpha q/11 Proteins Contribute to T Lymphocyte Migration by Promoting Turnover of Integrin LFA-1 through Recycling

Svensson, Lena M LU ; Stanley, Paula; Willenbrock, Frances and Hogg, Nancy (2012) In PLoS ONE 7(6).
Abstract
The role of G alpha i proteins coupled to chemokine receptors in directed migration of immune cells is well understood. In this study we show that the separate class of G alpha q/11 proteins is required for the underlying ability of T cells to migrate both randomly and in a directed chemokine-dependent manner. Interfering with G alpha q or G alpha 11 using dominant negative cDNA constructs or siRNA for G alpha q causes accumulation of LFA-1 adhesions and stalled migration. G alpha q/11 has an impact on LFA-1 expression at plasma membrane level and also on its internalization. Additionally G alpha q co-localizes with LFA-1- and EEA1-expressing intracellular vesicles and partially with Rap1- but not Rab11-expressing vesicles. However the... (More)
The role of G alpha i proteins coupled to chemokine receptors in directed migration of immune cells is well understood. In this study we show that the separate class of G alpha q/11 proteins is required for the underlying ability of T cells to migrate both randomly and in a directed chemokine-dependent manner. Interfering with G alpha q or G alpha 11 using dominant negative cDNA constructs or siRNA for G alpha q causes accumulation of LFA-1 adhesions and stalled migration. G alpha q/11 has an impact on LFA-1 expression at plasma membrane level and also on its internalization. Additionally G alpha q co-localizes with LFA-1- and EEA1-expressing intracellular vesicles and partially with Rap1- but not Rab11-expressing vesicles. However the influence of G alpha q is not confined to the vesicles that express it, as its reduction alters intracellular trafficking of other vesicles involved in recycling. In summary vesicle-associated G alpha q/11 is required for the turnover of LFA-1 adhesion that is necessary for migration. These G proteins participate directly in the initial phase of recycling and this has an impact on later stages of the endo-exocytic pathway. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
PLoS ONE
volume
7
issue
6
publisher
Public Library of Science
external identifiers
  • wos:000305337600028
  • scopus:84862206100
ISSN
1932-6203
DOI
10.1371/journal.pone.0038517
language
English
LU publication?
yes
id
980682f4-87a2-4b84-afe5-87d3c03325ca (old id 2891287)
date added to LUP
2012-07-25 17:28:32
date last changed
2017-11-19 03:57:01
@article{980682f4-87a2-4b84-afe5-87d3c03325ca,
  abstract     = {The role of G alpha i proteins coupled to chemokine receptors in directed migration of immune cells is well understood. In this study we show that the separate class of G alpha q/11 proteins is required for the underlying ability of T cells to migrate both randomly and in a directed chemokine-dependent manner. Interfering with G alpha q or G alpha 11 using dominant negative cDNA constructs or siRNA for G alpha q causes accumulation of LFA-1 adhesions and stalled migration. G alpha q/11 has an impact on LFA-1 expression at plasma membrane level and also on its internalization. Additionally G alpha q co-localizes with LFA-1- and EEA1-expressing intracellular vesicles and partially with Rap1- but not Rab11-expressing vesicles. However the influence of G alpha q is not confined to the vesicles that express it, as its reduction alters intracellular trafficking of other vesicles involved in recycling. In summary vesicle-associated G alpha q/11 is required for the turnover of LFA-1 adhesion that is necessary for migration. These G proteins participate directly in the initial phase of recycling and this has an impact on later stages of the endo-exocytic pathway.},
  author       = {Svensson, Lena M and Stanley, Paula and Willenbrock, Frances and Hogg, Nancy},
  issn         = {1932-6203},
  language     = {eng},
  number       = {6},
  publisher    = {Public Library of Science},
  series       = {PLoS ONE},
  title        = {The G alpha q/11 Proteins Contribute to T Lymphocyte Migration by Promoting Turnover of Integrin LFA-1 through Recycling},
  url          = {http://dx.doi.org/10.1371/journal.pone.0038517},
  volume       = {7},
  year         = {2012},
}