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Escherichia coli RNase E and RNase G cleave a Bacillus subtilis transcript at the same site in a structure-dependent manner

Hambraeus, Gustav LU and Rutberg, Blanka LU (2004) In Archives of Microbiology 181(2). p.137-143
Abstract
The decay of Bacillus subtilis aprE leader-lacZ mRNA was examined in Escherichia coli wild-type and in mutants deficient in RNase E, RNase G, or both. Two versions of the mRNA were studied: the wild-type mRNA, which has a stem-loop at the 5' end, and a mutant mRNA, with a single-stranded 5' end. The half-life of both transcripts was determined by RNase E, the half-life of the mutant transcript being one-third of that of the wild-type transcript. RNase G cleaved both transcripts at a site within an AU-rich sequence in the stem-loop region, but cleavage was much more efficient when the stem-loop was destabilized. RNase E cleaved at the same site, but less efficiently and only in the mutant transcript.
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
mRNA degradation, RNase E, RNase G
in
Archives of Microbiology
volume
181
issue
2
pages
137 - 143
publisher
Springer
external identifiers
  • wos:000188457000007
  • pmid:14685649
  • scopus:1242337413
ISSN
0302-8933
DOI
10.1007/s00203-003-0637-1
language
English
LU publication?
yes
id
152e0a3f-f54d-43cc-97ef-2ba28411a9ca (old id 289577)
date added to LUP
2007-10-29 11:06:58
date last changed
2017-09-03 04:40:37
@article{152e0a3f-f54d-43cc-97ef-2ba28411a9ca,
  abstract     = {The decay of Bacillus subtilis aprE leader-lacZ mRNA was examined in Escherichia coli wild-type and in mutants deficient in RNase E, RNase G, or both. Two versions of the mRNA were studied: the wild-type mRNA, which has a stem-loop at the 5' end, and a mutant mRNA, with a single-stranded 5' end. The half-life of both transcripts was determined by RNase E, the half-life of the mutant transcript being one-third of that of the wild-type transcript. RNase G cleaved both transcripts at a site within an AU-rich sequence in the stem-loop region, but cleavage was much more efficient when the stem-loop was destabilized. RNase E cleaved at the same site, but less efficiently and only in the mutant transcript.},
  author       = {Hambraeus, Gustav and Rutberg, Blanka},
  issn         = {0302-8933},
  keyword      = {mRNA degradation,RNase E,RNase G},
  language     = {eng},
  number       = {2},
  pages        = {137--143},
  publisher    = {Springer},
  series       = {Archives of Microbiology},
  title        = {Escherichia coli RNase E and RNase G cleave a Bacillus subtilis transcript at the same site in a structure-dependent manner},
  url          = {http://dx.doi.org/10.1007/s00203-003-0637-1},
  volume       = {181},
  year         = {2004},
}