Escherichia coli RNase E and RNase G cleave a Bacillus subtilis transcript at the same site in a structure-dependent manner
(2004) In Archives of Microbiology 181(2). p.137-143- Abstract
- The decay of Bacillus subtilis aprE leader-lacZ mRNA was examined in Escherichia coli wild-type and in mutants deficient in RNase E, RNase G, or both. Two versions of the mRNA were studied: the wild-type mRNA, which has a stem-loop at the 5' end, and a mutant mRNA, with a single-stranded 5' end. The half-life of both transcripts was determined by RNase E, the half-life of the mutant transcript being one-third of that of the wild-type transcript. RNase G cleaved both transcripts at a site within an AU-rich sequence in the stem-loop region, but cleavage was much more efficient when the stem-loop was destabilized. RNase E cleaved at the same site, but less efficiently and only in the mutant transcript.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/289577
- author
- Hambraeus, Gustav LU and Rutberg, Blanka LU
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- mRNA degradation, RNase E, RNase G
- in
- Archives of Microbiology
- volume
- 181
- issue
- 2
- pages
- 137 - 143
- publisher
- Springer
- external identifiers
-
- wos:000188457000007
- pmid:14685649
- scopus:1242337413
- ISSN
- 0302-8933
- DOI
- 10.1007/s00203-003-0637-1
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Department of Cell and Organism Biology (Closed 2011.) (011002100)
- id
- 152e0a3f-f54d-43cc-97ef-2ba28411a9ca (old id 289577)
- date added to LUP
- 2016-04-01 16:46:23
- date last changed
- 2022-01-28 22:01:24
@article{152e0a3f-f54d-43cc-97ef-2ba28411a9ca, abstract = {{The decay of Bacillus subtilis aprE leader-lacZ mRNA was examined in Escherichia coli wild-type and in mutants deficient in RNase E, RNase G, or both. Two versions of the mRNA were studied: the wild-type mRNA, which has a stem-loop at the 5' end, and a mutant mRNA, with a single-stranded 5' end. The half-life of both transcripts was determined by RNase E, the half-life of the mutant transcript being one-third of that of the wild-type transcript. RNase G cleaved both transcripts at a site within an AU-rich sequence in the stem-loop region, but cleavage was much more efficient when the stem-loop was destabilized. RNase E cleaved at the same site, but less efficiently and only in the mutant transcript.}}, author = {{Hambraeus, Gustav and Rutberg, Blanka}}, issn = {{0302-8933}}, keywords = {{mRNA degradation; RNase E; RNase G}}, language = {{eng}}, number = {{2}}, pages = {{137--143}}, publisher = {{Springer}}, series = {{Archives of Microbiology}}, title = {{Escherichia coli RNase E and RNase G cleave a Bacillus subtilis transcript at the same site in a structure-dependent manner}}, url = {{http://dx.doi.org/10.1007/s00203-003-0637-1}}, doi = {{10.1007/s00203-003-0637-1}}, volume = {{181}}, year = {{2004}}, }