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Survival and long distance migration of brain-derived precursor cells transplanted to adult rat retina

Blixt Wojciechowski, Anita LU ; Englund Johansson, Ulrica LU ; Lundberg, Cecilia LU and Warfvinge, Karin LU (2004) In Stem Cells 22(1). p.27-38
Abstract
Neural precursor cells transplanted to adult retina can integrate into the host. This is especially true when the neural precursor rat cell line RN33B is used. This cell line carries the reporter genes LacZ and green fluorescent protein (GFP). In grafted rat eyes, RN33B cells are localized from one eccentricity to the other of the host retina. In the present study, whole-mounted retinas were analyzed to obtain a more appropriate evaluation of the amount of transgene-expressing cells and the migratory capacity of these cells 3 and 8 weeks post-transplantation. Quantification was made of the number of beta-galactosidase- and GFP-expressing cells with a semiautomatized stereological cell counting system. With the same system, delineation of... (More)
Neural precursor cells transplanted to adult retina can integrate into the host. This is especially true when the neural precursor rat cell line RN33B is used. This cell line carries the reporter genes LacZ and green fluorescent protein (GFP). In grafted rat eyes, RN33B cells are localized from one eccentricity to the other of the host retina. In the present study, whole-mounted retinas were analyzed to obtain a more appropriate evaluation of the amount of transgene-expressing cells and the migratory capacity of these cells 3 and 8 weeks post-transplantation. Quantification was made of the number of beta-galactosidase- and GFP-expressing cells with a semiautomatized stereological cell counting system. With the same system, delineation of the distribution area of the grafted cells was also performed. At 3 weeks, 68% of the grafted eyes contained marker-expressing cells, whereas at 8 weeks only 35% of the eyes contained such cells. Counting of marker-expressing cells demonstrated a lower number of transgene-expressing cells at 3 weeks compared with 8 weeks post-transplantation. The distribution pattern of marker gene-expressing cells revealed cells occupying up to 21% at 3 weeks and up to 68% at 8 weeks of the entire host retina post-grafting. The precursor cells survived well in the adult retina although the most striking feature of the RN33B cell line was its extraordinary migratory capacity. This capability could be useful if precursor cells are used to deliver necessary genes or gene products that need to be distributed over a large diseased area. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
markers, cell, subretinal transplantation, brain-derived precursor cells, RN33B, whole-mounted retina, migration
in
Stem Cells
volume
22
issue
1
pages
27 - 38
publisher
AlphaMed Press
external identifiers
  • wos:000187497200004
  • scopus:0347511693
ISSN
1549-4918
language
English
LU publication?
yes
id
32f2d2b3-2979-442b-a42f-7e66364b0c50 (old id 291271)
alternative location
http://stemcells.alphamedpress.org/cgi/content/abstract/22/1/27
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=14688389&dopt=Abstract
date added to LUP
2007-09-24 09:17:00
date last changed
2017-01-01 07:09:39
@article{32f2d2b3-2979-442b-a42f-7e66364b0c50,
  abstract     = {Neural precursor cells transplanted to adult retina can integrate into the host. This is especially true when the neural precursor rat cell line RN33B is used. This cell line carries the reporter genes LacZ and green fluorescent protein (GFP). In grafted rat eyes, RN33B cells are localized from one eccentricity to the other of the host retina. In the present study, whole-mounted retinas were analyzed to obtain a more appropriate evaluation of the amount of transgene-expressing cells and the migratory capacity of these cells 3 and 8 weeks post-transplantation. Quantification was made of the number of beta-galactosidase- and GFP-expressing cells with a semiautomatized stereological cell counting system. With the same system, delineation of the distribution area of the grafted cells was also performed. At 3 weeks, 68% of the grafted eyes contained marker-expressing cells, whereas at 8 weeks only 35% of the eyes contained such cells. Counting of marker-expressing cells demonstrated a lower number of transgene-expressing cells at 3 weeks compared with 8 weeks post-transplantation. The distribution pattern of marker gene-expressing cells revealed cells occupying up to 21% at 3 weeks and up to 68% at 8 weeks of the entire host retina post-grafting. The precursor cells survived well in the adult retina although the most striking feature of the RN33B cell line was its extraordinary migratory capacity. This capability could be useful if precursor cells are used to deliver necessary genes or gene products that need to be distributed over a large diseased area.},
  author       = {Blixt Wojciechowski, Anita and Englund Johansson, Ulrica and Lundberg, Cecilia and Warfvinge, Karin},
  issn         = {1549-4918},
  keyword      = {markers,cell,subretinal transplantation,brain-derived precursor cells,RN33B,whole-mounted retina,migration},
  language     = {eng},
  number       = {1},
  pages        = {27--38},
  publisher    = {AlphaMed Press},
  series       = {Stem Cells},
  title        = {Survival and long distance migration of brain-derived precursor cells transplanted to adult rat retina},
  volume       = {22},
  year         = {2004},
}