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Divergence of Hoxc8 early enhancer parallels diverged axial morphologies between mammals and fishes

Anand, S; Wang, WCH; Powell, DR; Bolanowski, SA; Zhang, J; Ledje, Christina LU ; Pawashe, AB; Amemiya, CT and Shashikant, CS (2003) In Proceedings of the National Academy of Sciences 100(26). p.15666-15669
Abstract
There is considerable interest in understanding how cis-regulatory modifications drive morphological changes across species. Because developmental regulatory genes, including Hox genes, are remarkably conserved, their noncoding regulatory regions are likely sources for variations. Modifications of Hox cis-regulatory elements have potential to alter Hox gene expression and, hence, axial morphologies. In vertebrates, differences in the axial levels of Hox gene expression correlate with differences in the number and relative position of thoracic vertebrae. Variation in cis-regulatory elements of Hox genes can be identified by comparative sequence and reporter gene analyses in transgenic mouse embryos. Using these approaches, we show a... (More)
There is considerable interest in understanding how cis-regulatory modifications drive morphological changes across species. Because developmental regulatory genes, including Hox genes, are remarkably conserved, their noncoding regulatory regions are likely sources for variations. Modifications of Hox cis-regulatory elements have potential to alter Hox gene expression and, hence, axial morphologies. In vertebrates, differences in the axial levels of Hox gene expression correlate with differences in the number and relative position of thoracic vertebrae. Variation in cis-regulatory elements of Hox genes can be identified by comparative sequence and reporter gene analyses in transgenic mouse embryos. Using these approaches, we show a remarkable divergence of the Hoxc8 early enhancers between mammals and fishes representing diverse axial morphologies. Extensive restructuring of the Hoxc8 early enhancer including nucleotide substitutions, inversion, and divergence result in distinct patterns of reporter gene expression along the embryonic axis. Our results provide an evolutionary perspective on how the enhancer elements are engineered and support the hypothesis that remodeling of Hox regulatory elements in different species has played a significant role in generating morphological diversity. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Proceedings of the National Academy of Sciences
volume
100
issue
26
pages
15666 - 15669
publisher
National Acad Sciences
external identifiers
  • wos:000187554600069
  • scopus:0346734128
ISSN
1091-6490
DOI
10.1073/pnas.2535667100
language
English
LU publication?
yes
id
6022702e-991d-4eff-ba5a-3c6f5fa227b6 (old id 291276)
date added to LUP
2007-08-22 10:44:18
date last changed
2018-05-29 12:26:16
@article{6022702e-991d-4eff-ba5a-3c6f5fa227b6,
  abstract     = {There is considerable interest in understanding how cis-regulatory modifications drive morphological changes across species. Because developmental regulatory genes, including Hox genes, are remarkably conserved, their noncoding regulatory regions are likely sources for variations. Modifications of Hox cis-regulatory elements have potential to alter Hox gene expression and, hence, axial morphologies. In vertebrates, differences in the axial levels of Hox gene expression correlate with differences in the number and relative position of thoracic vertebrae. Variation in cis-regulatory elements of Hox genes can be identified by comparative sequence and reporter gene analyses in transgenic mouse embryos. Using these approaches, we show a remarkable divergence of the Hoxc8 early enhancers between mammals and fishes representing diverse axial morphologies. Extensive restructuring of the Hoxc8 early enhancer including nucleotide substitutions, inversion, and divergence result in distinct patterns of reporter gene expression along the embryonic axis. Our results provide an evolutionary perspective on how the enhancer elements are engineered and support the hypothesis that remodeling of Hox regulatory elements in different species has played a significant role in generating morphological diversity.},
  author       = {Anand, S and Wang, WCH and Powell, DR and Bolanowski, SA and Zhang, J and Ledje, Christina and Pawashe, AB and Amemiya, CT and Shashikant, CS},
  issn         = {1091-6490},
  language     = {eng},
  number       = {26},
  pages        = {15666--15669},
  publisher    = {National Acad Sciences},
  series       = {Proceedings of the National Academy of Sciences},
  title        = {Divergence of Hoxc8 early enhancer parallels diverged axial morphologies between mammals and fishes},
  url          = {http://dx.doi.org/10.1073/pnas.2535667100},
  volume       = {100},
  year         = {2003},
}