Divergence of Hoxc8 early enhancer parallels diverged axial morphologies between mammals and fishes
(2003) In Proceedings of the National Academy of Sciences 100(26). p.15666-15669- Abstract
- There is considerable interest in understanding how cis-regulatory modifications drive morphological changes across species. Because developmental regulatory genes, including Hox genes, are remarkably conserved, their noncoding regulatory regions are likely sources for variations. Modifications of Hox cis-regulatory elements have potential to alter Hox gene expression and, hence, axial morphologies. In vertebrates, differences in the axial levels of Hox gene expression correlate with differences in the number and relative position of thoracic vertebrae. Variation in cis-regulatory elements of Hox genes can be identified by comparative sequence and reporter gene analyses in transgenic mouse embryos. Using these approaches, we show a... (More)
- There is considerable interest in understanding how cis-regulatory modifications drive morphological changes across species. Because developmental regulatory genes, including Hox genes, are remarkably conserved, their noncoding regulatory regions are likely sources for variations. Modifications of Hox cis-regulatory elements have potential to alter Hox gene expression and, hence, axial morphologies. In vertebrates, differences in the axial levels of Hox gene expression correlate with differences in the number and relative position of thoracic vertebrae. Variation in cis-regulatory elements of Hox genes can be identified by comparative sequence and reporter gene analyses in transgenic mouse embryos. Using these approaches, we show a remarkable divergence of the Hoxc8 early enhancers between mammals and fishes representing diverse axial morphologies. Extensive restructuring of the Hoxc8 early enhancer including nucleotide substitutions, inversion, and divergence result in distinct patterns of reporter gene expression along the embryonic axis. Our results provide an evolutionary perspective on how the enhancer elements are engineered and support the hypothesis that remodeling of Hox regulatory elements in different species has played a significant role in generating morphological diversity. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/291276
- author
- Anand, S ; Wang, WCH ; Powell, DR ; Bolanowski, SA ; Zhang, J ; Ledje, Christina LU ; Pawashe, AB ; Amemiya, CT and Shashikant, CS
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Proceedings of the National Academy of Sciences
- volume
- 100
- issue
- 26
- pages
- 15666 - 15669
- publisher
- National Academy of Sciences
- external identifiers
-
- wos:000187554600069
- scopus:0346734128
- ISSN
- 1091-6490
- DOI
- 10.1073/pnas.2535667100
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Genetics (Closed 2011) (011005100)
- id
- 6022702e-991d-4eff-ba5a-3c6f5fa227b6 (old id 291276)
- date added to LUP
- 2016-04-01 12:26:56
- date last changed
- 2022-01-27 03:55:06
@article{6022702e-991d-4eff-ba5a-3c6f5fa227b6,
abstract = {{There is considerable interest in understanding how cis-regulatory modifications drive morphological changes across species. Because developmental regulatory genes, including Hox genes, are remarkably conserved, their noncoding regulatory regions are likely sources for variations. Modifications of Hox cis-regulatory elements have potential to alter Hox gene expression and, hence, axial morphologies. In vertebrates, differences in the axial levels of Hox gene expression correlate with differences in the number and relative position of thoracic vertebrae. Variation in cis-regulatory elements of Hox genes can be identified by comparative sequence and reporter gene analyses in transgenic mouse embryos. Using these approaches, we show a remarkable divergence of the Hoxc8 early enhancers between mammals and fishes representing diverse axial morphologies. Extensive restructuring of the Hoxc8 early enhancer including nucleotide substitutions, inversion, and divergence result in distinct patterns of reporter gene expression along the embryonic axis. Our results provide an evolutionary perspective on how the enhancer elements are engineered and support the hypothesis that remodeling of Hox regulatory elements in different species has played a significant role in generating morphological diversity.}},
author = {{Anand, S and Wang, WCH and Powell, DR and Bolanowski, SA and Zhang, J and Ledje, Christina and Pawashe, AB and Amemiya, CT and Shashikant, CS}},
issn = {{1091-6490}},
language = {{eng}},
number = {{26}},
pages = {{15666--15669}},
publisher = {{National Academy of Sciences}},
series = {{Proceedings of the National Academy of Sciences}},
title = {{Divergence of Hoxc8 early enhancer parallels diverged axial morphologies between mammals and fishes}},
url = {{http://dx.doi.org/10.1073/pnas.2535667100}},
doi = {{10.1073/pnas.2535667100}},
volume = {{100}},
year = {{2003}},
}