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Levels of DNA damage (Micronuclei) in patients suffering from chronic kidney disease. Role of GST polymorphisms

Pastor, Susana; Rodríguez-Ribera, Lara; Corredor, Zuray; da Silva Filho, Miguel Inácio LU ; Hemminki, Kari LU ; Coll, Elisabeth; Försti, Asta LU and Marcos, Ricard (2018) In Mutation Research - Genetic Toxicology and Environmental Mutagenesis 836. p.41-46
Abstract

Chronic kidney disease (CKD) patients are characterized by the presence of high levels of DNA damage, and a poor response to ionizing radiation. In this study, we proposed that variants in GST genes could explain this fact. One-hundred twenty seven CKD patients and one-hundred forty five controls constituted the studied groups. Micronuclei (MN) frequency was determined in peripheral blood lymphocytes at both basal level, and after challenging the cells with 0.5 Gy of ionizing radiation. The following polymorphisms: GSTP1 (rs749174), GSTO1 (rs2164624), and GSTO2 (rs156697) were evaluated in the two groups. Results indicate that gene variants were distributed differentially between CKD patients and controls. Although GSTO1 and GSTO2... (More)

Chronic kidney disease (CKD) patients are characterized by the presence of high levels of DNA damage, and a poor response to ionizing radiation. In this study, we proposed that variants in GST genes could explain this fact. One-hundred twenty seven CKD patients and one-hundred forty five controls constituted the studied groups. Micronuclei (MN) frequency was determined in peripheral blood lymphocytes at both basal level, and after challenging the cells with 0.5 Gy of ionizing radiation. The following polymorphisms: GSTP1 (rs749174), GSTO1 (rs2164624), and GSTO2 (rs156697) were evaluated in the two groups. Results indicate that gene variants were distributed differentially between CKD patients and controls. Although GSTO1 and GSTO2 variants were associated with lower levels of MN, this was observed in both CKD patients and controls. When net MN values were determined after irradiation, GSTO1 and GSTO2 variants were also associated with lower MN-frequencies. On the contrary, individuals with the GSTP1 variant showed higher values of induced MN. In conclusion, we have demonstrate that the selected GST polymorphism play a role in the incidence of CKD, and affects the levels of MN. Interestingly, the modulating effects observed on both, the basal and induced levels of DNA damage, are characteristic of the overall population, not only of the CKD patients.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
CKD patients, GST polymorphisms, Micronuclei, Radiosensitivity, Single nucleotide polymorphisms
in
Mutation Research - Genetic Toxicology and Environmental Mutagenesis
volume
836
pages
41 - 46
publisher
Elsevier
external identifiers
  • scopus:85046830581
ISSN
1383-5718
DOI
10.1016/j.mrgentox.2018.05.008
language
English
LU publication?
yes
id
292840e1-2dcf-4f3e-8206-a3461ee547a0
date added to LUP
2018-05-25 13:56:38
date last changed
2019-03-19 03:55:21
@article{292840e1-2dcf-4f3e-8206-a3461ee547a0,
  abstract     = {<p>Chronic kidney disease (CKD) patients are characterized by the presence of high levels of DNA damage, and a poor response to ionizing radiation. In this study, we proposed that variants in GST genes could explain this fact. One-hundred twenty seven CKD patients and one-hundred forty five controls constituted the studied groups. Micronuclei (MN) frequency was determined in peripheral blood lymphocytes at both basal level, and after challenging the cells with 0.5 Gy of ionizing radiation. The following polymorphisms: GSTP1 (rs749174), GSTO1 (rs2164624), and GSTO2 (rs156697) were evaluated in the two groups. Results indicate that gene variants were distributed differentially between CKD patients and controls. Although GSTO1 and GSTO2 variants were associated with lower levels of MN, this was observed in both CKD patients and controls. When net MN values were determined after irradiation, GSTO1 and GSTO2 variants were also associated with lower MN-frequencies. On the contrary, individuals with the GSTP1 variant showed higher values of induced MN. In conclusion, we have demonstrate that the selected GST polymorphism play a role in the incidence of CKD, and affects the levels of MN. Interestingly, the modulating effects observed on both, the basal and induced levels of DNA damage, are characteristic of the overall population, not only of the CKD patients.</p>},
  author       = {Pastor, Susana and Rodríguez-Ribera, Lara and Corredor, Zuray and da Silva Filho, Miguel Inácio and Hemminki, Kari and Coll, Elisabeth and Försti, Asta and Marcos, Ricard},
  issn         = {1383-5718},
  keyword      = {CKD patients,GST polymorphisms,Micronuclei,Radiosensitivity,Single nucleotide polymorphisms},
  language     = {eng},
  month        = {05},
  pages        = {41--46},
  publisher    = {Elsevier},
  series       = {Mutation Research - Genetic Toxicology and Environmental Mutagenesis},
  title        = {Levels of DNA damage (Micronuclei) in patients suffering from chronic kidney disease. Role of GST polymorphisms},
  url          = {http://dx.doi.org/10.1016/j.mrgentox.2018.05.008},
  volume       = {836},
  year         = {2018},
}