Identification and use of personalized genomic markers for monitoring circulating tumor DNA
Chen, Yilun LU ; George, Anthony M. LU ; Olsson, Eleonor LU and Saal, Lao H. LU
(2018)
In Methods in Molecular Biology
1768.
p.303-322
- Abstract
Digital PCR techniques are ideally suited for accurately quantifying trace amounts of target DNA sequences, such as tumor-derived mutant DNA that is present in the blood circulation of patients with cancer. Here, we describe an approach marrying low-coverage whole-genome sequencing of tumor tissues, to enumerate chromosomal rearrangement breakpoints, together with droplet digital PCR (ddPCR)-based personalized rearrangement assays to cost-effectively monitor circulating tumor DNA levels at multiple time-points during the clinical course. The method is generally applicable to essentially any cancer patient, as all cancers harbor unstable genomes, and may have uses for measuring minimal residual disease, response to therapy, and early... (More)
Digital PCR techniques are ideally suited for accurately quantifying trace amounts of target DNA sequences, such as tumor-derived mutant DNA that is present in the blood circulation of patients with cancer. Here, we describe an approach marrying low-coverage whole-genome sequencing of tumor tissues, to enumerate chromosomal rearrangement breakpoints, together with droplet digital PCR (ddPCR)-based personalized rearrangement assays to cost-effectively monitor circulating tumor DNA levels at multiple time-points during the clinical course. The method is generally applicable to essentially any cancer patient, as all cancers harbor unstable genomes, and may have uses for measuring minimal residual disease, response to therapy, and early detection of metastasis.
(Less)
- author
- Chen, Yilun
LU
; George, Anthony M.
LU
; Olsson, Eleonor
LU
and Saal, Lao H.
LU
- organization
- publishing date
- 2018-01-01
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- Cell-free circulating tumor DNA, Droplet digital PCR, Liquid biopsy, Noninvasive diagnosis, Personalized medicine, Whole-genome sequencing
- host publication
- Methods in Molecular Biology
- series title
- Methods in Molecular Biology
- volume
- 1768
- pages
- 20 pages
- publisher
- Humana Press
- external identifiers
-
- scopus:85046362164
- pmid:29717450
- ISSN
- 1064-3745
- DOI
- 10.1007/978-1-4939-7778-9_17
- project
- Translational development and clinical applications of circulating tumor DNA for patient stratification, therapy guidance, and disease monitoring
- language
- English
- LU publication?
- yes
- id
- 292b7066-a4e0-4712-8a3e-8381c13467b0
- date added to LUP
- 2018-05-17 14:34:24
- date last changed
- 2024-01-29 16:10:43
@inbook{292b7066-a4e0-4712-8a3e-8381c13467b0,
abstract = {{<p>Digital PCR techniques are ideally suited for accurately quantifying trace amounts of target DNA sequences, such as tumor-derived mutant DNA that is present in the blood circulation of patients with cancer. Here, we describe an approach marrying low-coverage whole-genome sequencing of tumor tissues, to enumerate chromosomal rearrangement breakpoints, together with droplet digital PCR (ddPCR)-based personalized rearrangement assays to cost-effectively monitor circulating tumor DNA levels at multiple time-points during the clinical course. The method is generally applicable to essentially any cancer patient, as all cancers harbor unstable genomes, and may have uses for measuring minimal residual disease, response to therapy, and early detection of metastasis.</p>}},
author = {{Chen, Yilun and George, Anthony M. and Olsson, Eleonor and Saal, Lao H.}},
booktitle = {{Methods in Molecular Biology}},
issn = {{1064-3745}},
keywords = {{Cell-free circulating tumor DNA; Droplet digital PCR; Liquid biopsy; Noninvasive diagnosis; Personalized medicine; Whole-genome sequencing}},
language = {{eng}},
month = {{01}},
pages = {{303--322}},
publisher = {{Humana Press}},
series = {{Methods in Molecular Biology}},
title = {{Identification and use of personalized genomic markers for monitoring circulating tumor DNA}},
url = {{http://dx.doi.org/10.1007/978-1-4939-7778-9_17}},
doi = {{10.1007/978-1-4939-7778-9_17}},
volume = {{1768}},
year = {{2018}},
}