Regulation of Drosophila Jun by phosphorylation and the ubiquitin system during eye development
(1997)- Abstract
- The goal of these studies has been to elucidate the biological relevance of post-translational modifications in the context of a living organism. The model system used is photoreceptor differentiation during development of the compound eye in the fruit fly Drosophila melanogaster. Important signal transduction pathways in this process consist of receptor tyrosine kinases (RTKs) coupled to a mitogen activated protein kinase cascade. In the nucleus several transcription factors have been implicated as mediators of the pathway.
The first part of the work presented here focuses on the role of signal-dependent phosporylation of D-Jun in photoreceptor differentiation. It appears that Rolled is the only kinase phosphorylating... (More) - The goal of these studies has been to elucidate the biological relevance of post-translational modifications in the context of a living organism. The model system used is photoreceptor differentiation during development of the compound eye in the fruit fly Drosophila melanogaster. Important signal transduction pathways in this process consist of receptor tyrosine kinases (RTKs) coupled to a mitogen activated protein kinase cascade. In the nucleus several transcription factors have been implicated as mediators of the pathway.
The first part of the work presented here focuses on the role of signal-dependent phosporylation of D-Jun in photoreceptor differentiation. It appears that Rolled is the only kinase phosphorylating D-Jun during eye development. Interestingly, Rolled is a kinase of the extracellular signal reulated (ERK) sub-type of kinases, in contrast to the c-Jun N-terminal kiases (JNKs), which phosphorylate c-Jun in many cell culture systems. Phosphorylation by Rolled appears to be important for D-Jun function, since a D-Jun protein with the phosphorylation sites mutated to the non-phosphorylatable amino acid alanine can suppress formation of ectopic photoreceptors.
Another study addresses the role of ubiquitin- dependent proteolysis in photoreceptor differentiation. A loss-of-function mutation in a gene involved in ubuquitin-dependent proteolysis (fat facets) causes a zygotic phenotype with 1-2 ectopic photoreceptors per unit eye. Genetic interactions with components of the neurogenic signal transduction pathway suggest that the pathway is hyperactivated in the faf mutants. This idea is consistent with the observed increased level and prolonged expression of D-Jun protein in faf eye imaginal discs. Since reducing D-Jun activity suppresses the formation of ectopic photoreceptors caused by faf, it is likely that the increased expression of D-Jun contributes to the phenotype. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/29525
- author
- Isaksson, Anders LU
- supervisor
- opponent
-
- Dr Freeman, Matthew, Cambridge, UK
- organization
- publishing date
- 1997
- type
- Thesis
- publication status
- published
- subject
- keywords
- Genetics, eye development, ubiquitin, D-Jun, phosphorylation, cytogenetics, Genetik, cytogenetik
- pages
- 100 pages
- publisher
- Department of Genetics, Lund University
- defense location
- Sölvegatan 29
- defense date
- 1997-10-15 10:00:00
- external identifiers
-
- other:ISRN: LUNBDS/NBGE 1028/001-100
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Genetics (Closed 2011) (011005100)
- id
- 691c501c-93e8-4a02-b032-e1295ed2bc8a (old id 29525)
- date added to LUP
- 2016-04-04 11:57:50
- date last changed
- 2018-11-21 21:08:13
@phdthesis{691c501c-93e8-4a02-b032-e1295ed2bc8a,
abstract = {{The goal of these studies has been to elucidate the biological relevance of post-translational modifications in the context of a living organism. The model system used is photoreceptor differentiation during development of the compound eye in the fruit fly Drosophila melanogaster. Important signal transduction pathways in this process consist of receptor tyrosine kinases (RTKs) coupled to a mitogen activated protein kinase cascade. In the nucleus several transcription factors have been implicated as mediators of the pathway.<br/><br>
<br/><br>
The first part of the work presented here focuses on the role of signal-dependent phosporylation of D-Jun in photoreceptor differentiation. It appears that Rolled is the only kinase phosphorylating D-Jun during eye development. Interestingly, Rolled is a kinase of the extracellular signal reulated (ERK) sub-type of kinases, in contrast to the c-Jun N-terminal kiases (JNKs), which phosphorylate c-Jun in many cell culture systems. Phosphorylation by Rolled appears to be important for D-Jun function, since a D-Jun protein with the phosphorylation sites mutated to the non-phosphorylatable amino acid alanine can suppress formation of ectopic photoreceptors.<br/><br>
<br/><br>
Another study addresses the role of ubiquitin- dependent proteolysis in photoreceptor differentiation. A loss-of-function mutation in a gene involved in ubuquitin-dependent proteolysis (fat facets) causes a zygotic phenotype with 1-2 ectopic photoreceptors per unit eye. Genetic interactions with components of the neurogenic signal transduction pathway suggest that the pathway is hyperactivated in the faf mutants. This idea is consistent with the observed increased level and prolonged expression of D-Jun protein in faf eye imaginal discs. Since reducing D-Jun activity suppresses the formation of ectopic photoreceptors caused by faf, it is likely that the increased expression of D-Jun contributes to the phenotype.}},
author = {{Isaksson, Anders}},
keywords = {{Genetics; eye development; ubiquitin; D-Jun; phosphorylation; cytogenetics; Genetik; cytogenetik}},
language = {{eng}},
publisher = {{Department of Genetics, Lund University}},
school = {{Lund University}},
title = {{Regulation of Drosophila Jun by phosphorylation and the ubiquitin system during eye development}},
year = {{1997}},
}