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Phase behavior and rheological properties of DNA-cationic polysaccharide mixtures.

Santos, Salome LU ; Piculell, Lennart LU ; Medronho, Bruno LU ; Miguel, Maria LU and Lindman, Björn LU (2012) In Journal of Colloid and Interface Science 383(1). p.63-74
Abstract
Associative aqueous mixtures over a range of concentrations of double- (ds) or single- (ss) stranded DNA with dilute or semidilute solutions of two cationic derivatives of hydroxyethyl cellulose (cat-HEC and cat-HMHEC,(1) the latter carrying grafted hydrophobic groups), were studied. The phase behavior showed an interesting asymmetry: Phase separation occurred immediately when small (sub-stoichiometric) amounts of cationic polyelectrolyte were added to the DNA solution, but redissolution into a single cat-(HM)HEC/DNA/H(2)O phase occurred already with a modest charge excess of the cationic polyelectrolyte, at a charge ratio approximately independent of the overall polyelectrolyte concentration. Cat-HEC/dsDNA/H(2)O and cat-HEC/ssDNA/H(2)O... (More)
Associative aqueous mixtures over a range of concentrations of double- (ds) or single- (ss) stranded DNA with dilute or semidilute solutions of two cationic derivatives of hydroxyethyl cellulose (cat-HEC and cat-HMHEC,(1) the latter carrying grafted hydrophobic groups), were studied. The phase behavior showed an interesting asymmetry: Phase separation occurred immediately when small (sub-stoichiometric) amounts of cationic polyelectrolyte were added to the DNA solution, but redissolution into a single cat-(HM)HEC/DNA/H(2)O phase occurred already with a modest charge excess of the cationic polyelectrolyte, at a charge ratio approximately independent of the overall polyelectrolyte concentration. Cat-HEC/dsDNA/H(2)O and cat-HEC/ssDNA/H(2)O systems presented a considerable difference in the extension of the phase separation region. The one-phase samples with excess cationic polyelectrolyte were studied by rheology. The presence of DNA strengthened the viscoelastic behavior of the solutions of the cationic polyelectrolytes, reflected in an increase in storage modulus and viscosity. Differences in phase behavior and rheology were observed, particularly between systems containing cat-HEC or cat-HMHEC, but also between dsDNA and ssDNA. Thus, these systems allow for the preparation of DNA formulations with widely variable rheology and water uptake. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Colloid and Interface Science
volume
383
issue
1
pages
63 - 74
publisher
Elsevier
external identifiers
  • wos:000307207300009
  • pmid:22795045
  • scopus:84864103513
ISSN
1095-7103
DOI
10.1016/j.jcis.2012.06.011
language
English
LU publication?
yes
id
c60359bb-30fb-4a32-8663-6c294963e88d (old id 2967168)
date added to LUP
2012-09-05 09:38:02
date last changed
2017-10-22 03:17:53
@article{c60359bb-30fb-4a32-8663-6c294963e88d,
  abstract     = {Associative aqueous mixtures over a range of concentrations of double- (ds) or single- (ss) stranded DNA with dilute or semidilute solutions of two cationic derivatives of hydroxyethyl cellulose (cat-HEC and cat-HMHEC,(1) the latter carrying grafted hydrophobic groups), were studied. The phase behavior showed an interesting asymmetry: Phase separation occurred immediately when small (sub-stoichiometric) amounts of cationic polyelectrolyte were added to the DNA solution, but redissolution into a single cat-(HM)HEC/DNA/H(2)O phase occurred already with a modest charge excess of the cationic polyelectrolyte, at a charge ratio approximately independent of the overall polyelectrolyte concentration. Cat-HEC/dsDNA/H(2)O and cat-HEC/ssDNA/H(2)O systems presented a considerable difference in the extension of the phase separation region. The one-phase samples with excess cationic polyelectrolyte were studied by rheology. The presence of DNA strengthened the viscoelastic behavior of the solutions of the cationic polyelectrolytes, reflected in an increase in storage modulus and viscosity. Differences in phase behavior and rheology were observed, particularly between systems containing cat-HEC or cat-HMHEC, but also between dsDNA and ssDNA. Thus, these systems allow for the preparation of DNA formulations with widely variable rheology and water uptake.},
  author       = {Santos, Salome and Piculell, Lennart and Medronho, Bruno and Miguel, Maria and Lindman, Björn},
  issn         = {1095-7103},
  language     = {eng},
  number       = {1},
  pages        = {63--74},
  publisher    = {Elsevier},
  series       = {Journal of Colloid and Interface Science},
  title        = {Phase behavior and rheological properties of DNA-cationic polysaccharide mixtures.},
  url          = {http://dx.doi.org/10.1016/j.jcis.2012.06.011},
  volume       = {383},
  year         = {2012},
}