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Use of human amelogenin in molecular encapsulation for the design of pH responsive microparticles

Svensson Bonde, Johan LU and Bülow, Leif LU (2012) In BMC Biotechnology 12.
Abstract
Background: Proteins can be used in drug delivery systems to improve pharmacological properties of an active substance. Differences in pH between tissues can be utilized in order to achieve a targeted drug release at a specific location or tissue, such as a tumor. The enamel matrix protein amelogenin has a pH dependent solubility profile and self-assemble to form aggregates at neutral pH. This could make amelogenin useful in the design of pH responsive drug delivery systems. Results: In this study amelogenin was evaluated as a pH responsive component in drug delivery applications. This was achieved by testing the ability of amelogenin to entrap/release other proteins upon changes in pH, and by testing if amelogenin could confer pH... (More)
Background: Proteins can be used in drug delivery systems to improve pharmacological properties of an active substance. Differences in pH between tissues can be utilized in order to achieve a targeted drug release at a specific location or tissue, such as a tumor. The enamel matrix protein amelogenin has a pH dependent solubility profile and self-assemble to form aggregates at neutral pH. This could make amelogenin useful in the design of pH responsive drug delivery systems. Results: In this study amelogenin was evaluated as a pH responsive component in drug delivery applications. This was achieved by testing the ability of amelogenin to entrap/release other proteins upon changes in pH, and by testing if amelogenin could confer pH responsiveness to an existing and versatile drug delivery system, such as gelatin microparticles. Amelogenin was able to encapsulate bovine serum albumin and insulin, which were used as model target proteins. The composite aggregates of amelogenin and target protein were formed at neutral pH and could be reversibly solubilized at weakly acidic pH. Gelatin microparticles prepared in the presence of amelogenin, showed a modulated structure in response to pH change, when studied by scanning electron microscopy, compared to particles without amelogenin. At neutral pH amelogenin induced formation of pores in the particle surface, which were not present at acidic pH, or in particles lacking amelogenin. Conclusions: The results from this study demonstrate that amelogenin can be a useful component in drug delivery systems in order to achieve a pH dependent response. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
BMC Biotechnology
volume
12
publisher
BioMed Central
external identifiers
  • wos:000306660900001
  • scopus:84861367668
ISSN
1472-6750
DOI
10.1186/1472-6750-12-25
language
English
LU publication?
yes
id
28e0fff9-b69a-4c3f-b8b0-4a49fa83b9ca (old id 2991485)
date added to LUP
2012-08-22 15:32:31
date last changed
2017-11-19 03:59:06
@article{28e0fff9-b69a-4c3f-b8b0-4a49fa83b9ca,
  abstract     = {Background: Proteins can be used in drug delivery systems to improve pharmacological properties of an active substance. Differences in pH between tissues can be utilized in order to achieve a targeted drug release at a specific location or tissue, such as a tumor. The enamel matrix protein amelogenin has a pH dependent solubility profile and self-assemble to form aggregates at neutral pH. This could make amelogenin useful in the design of pH responsive drug delivery systems. Results: In this study amelogenin was evaluated as a pH responsive component in drug delivery applications. This was achieved by testing the ability of amelogenin to entrap/release other proteins upon changes in pH, and by testing if amelogenin could confer pH responsiveness to an existing and versatile drug delivery system, such as gelatin microparticles. Amelogenin was able to encapsulate bovine serum albumin and insulin, which were used as model target proteins. The composite aggregates of amelogenin and target protein were formed at neutral pH and could be reversibly solubilized at weakly acidic pH. Gelatin microparticles prepared in the presence of amelogenin, showed a modulated structure in response to pH change, when studied by scanning electron microscopy, compared to particles without amelogenin. At neutral pH amelogenin induced formation of pores in the particle surface, which were not present at acidic pH, or in particles lacking amelogenin. Conclusions: The results from this study demonstrate that amelogenin can be a useful component in drug delivery systems in order to achieve a pH dependent response.},
  author       = {Svensson Bonde, Johan and Bülow, Leif},
  issn         = {1472-6750},
  language     = {eng},
  publisher    = {BioMed Central},
  series       = {BMC Biotechnology},
  title        = {Use of human amelogenin in molecular encapsulation for the design of pH responsive microparticles},
  url          = {http://dx.doi.org/10.1186/1472-6750-12-25},
  volume       = {12},
  year         = {2012},
}