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Recombinant human insulin-like growth factor-I stimulates in vitro matrix synthesis and cell proliferation in rabbit flexor tendon

Abrahamsson, S. O. LU ; Lundborg, G. LU and Lohmander, L. S. (1991) In Journal of Orthopaedic Research 9(4). p.495-502
Abstract

Flexor tendons have an intrinsic ability for repair, with a capacity to metabolize matrix components and to proliferate. To identify factors with the potential of affecting those abilities, the effects of recombinant human insulin-like growth factor (rhIGF-I), insulin and fetal calf serum (FCS) on the synthesis of proteoglycan, collagen, and non-collagen protein and cell proliferation were investigated in short-term explant cultures of the deep flexor tendon of the rabbit. Matrix synthesis and cell proliferation were stimulated dose dependently by rhIGF-I at doses between 10 and 250 and at 10-100 ng/ml, respectively, by insulin at 250-5,000 ng/ml, and by FCS at 2-15%. Estimated maximal stimulation (E(max)) of up to three times the... (More)

Flexor tendons have an intrinsic ability for repair, with a capacity to metabolize matrix components and to proliferate. To identify factors with the potential of affecting those abilities, the effects of recombinant human insulin-like growth factor (rhIGF-I), insulin and fetal calf serum (FCS) on the synthesis of proteoglycan, collagen, and non-collagen protein and cell proliferation were investigated in short-term explant cultures of the deep flexor tendon of the rabbit. Matrix synthesis and cell proliferation were stimulated dose dependently by rhIGF-I at doses between 10 and 250 and at 10-100 ng/ml, respectively, by insulin at 250-5,000 ng/ml, and by FCS at 2-15%. Estimated maximal stimulation (E(max)) of up to three times the control value was observed with rhIGF-I at 250 ng/ml. Maximal stimulation was observed at 5,000 ng/ml with insulin, and FCS at 15%. rhIGF-I was more potent than insulin in stimulating protein synthesis and cell proliferation. The E(max) of stimulation of proteoglycan and collagen synthesis by rhIGF-I were two times that of FCS, and the E(max) of cell proliferation by FCS was twice that of rhIGF-I. Growth factors thus have the ability to stimulate matrix synthesis and cell proliferation in rabbit flexor tendon. This provides a rationale for further studies on the role of growth factors in flexor tendon healing in humans.

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type
Contribution to journal
publication status
published
subject
in
Journal of Orthopaedic Research
volume
9
issue
4
pages
8 pages
publisher
John Wiley & Sons Inc.
external identifiers
  • pmid:2045976
  • scopus:0026198752
ISSN
0736-0266
language
English
LU publication?
no
id
29b86909-700a-46b1-8e80-13cafb6dba4b
date added to LUP
2016-05-04 18:15:44
date last changed
2024-02-18 17:07:47
@article{29b86909-700a-46b1-8e80-13cafb6dba4b,
  abstract     = {{<p>Flexor tendons have an intrinsic ability for repair, with a capacity to metabolize matrix components and to proliferate. To identify factors with the potential of affecting those abilities, the effects of recombinant human insulin-like growth factor (rhIGF-I), insulin and fetal calf serum (FCS) on the synthesis of proteoglycan, collagen, and non-collagen protein and cell proliferation were investigated in short-term explant cultures of the deep flexor tendon of the rabbit. Matrix synthesis and cell proliferation were stimulated dose dependently by rhIGF-I at doses between 10 and 250 and at 10-100 ng/ml, respectively, by insulin at 250-5,000 ng/ml, and by FCS at 2-15%. Estimated maximal stimulation (E(max)) of up to three times the control value was observed with rhIGF-I at 250 ng/ml. Maximal stimulation was observed at 5,000 ng/ml with insulin, and FCS at 15%. rhIGF-I was more potent than insulin in stimulating protein synthesis and cell proliferation. The E(max) of stimulation of proteoglycan and collagen synthesis by rhIGF-I were two times that of FCS, and the E(max) of cell proliferation by FCS was twice that of rhIGF-I. Growth factors thus have the ability to stimulate matrix synthesis and cell proliferation in rabbit flexor tendon. This provides a rationale for further studies on the role of growth factors in flexor tendon healing in humans.</p>}},
  author       = {{Abrahamsson, S. O. and Lundborg, G. and Lohmander, L. S.}},
  issn         = {{0736-0266}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{495--502}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Journal of Orthopaedic Research}},
  title        = {{Recombinant human insulin-like growth factor-I stimulates in vitro matrix synthesis and cell proliferation in rabbit flexor tendon}},
  volume       = {{9}},
  year         = {{1991}},
}