Recombinant human insulin-like growth factor-I stimulates in vitro matrix synthesis and cell proliferation in rabbit flexor tendon
(1991) In Journal of Orthopaedic Research 9(4). p.495-502- Abstract
Flexor tendons have an intrinsic ability for repair, with a capacity to metabolize matrix components and to proliferate. To identify factors with the potential of affecting those abilities, the effects of recombinant human insulin-like growth factor (rhIGF-I), insulin and fetal calf serum (FCS) on the synthesis of proteoglycan, collagen, and non-collagen protein and cell proliferation were investigated in short-term explant cultures of the deep flexor tendon of the rabbit. Matrix synthesis and cell proliferation were stimulated dose dependently by rhIGF-I at doses between 10 and 250 and at 10-100 ng/ml, respectively, by insulin at 250-5,000 ng/ml, and by FCS at 2-15%. Estimated maximal stimulation (E(max)) of up to three times the... (More)
Flexor tendons have an intrinsic ability for repair, with a capacity to metabolize matrix components and to proliferate. To identify factors with the potential of affecting those abilities, the effects of recombinant human insulin-like growth factor (rhIGF-I), insulin and fetal calf serum (FCS) on the synthesis of proteoglycan, collagen, and non-collagen protein and cell proliferation were investigated in short-term explant cultures of the deep flexor tendon of the rabbit. Matrix synthesis and cell proliferation were stimulated dose dependently by rhIGF-I at doses between 10 and 250 and at 10-100 ng/ml, respectively, by insulin at 250-5,000 ng/ml, and by FCS at 2-15%. Estimated maximal stimulation (E(max)) of up to three times the control value was observed with rhIGF-I at 250 ng/ml. Maximal stimulation was observed at 5,000 ng/ml with insulin, and FCS at 15%. rhIGF-I was more potent than insulin in stimulating protein synthesis and cell proliferation. The E(max) of stimulation of proteoglycan and collagen synthesis by rhIGF-I were two times that of FCS, and the E(max) of cell proliferation by FCS was twice that of rhIGF-I. Growth factors thus have the ability to stimulate matrix synthesis and cell proliferation in rabbit flexor tendon. This provides a rationale for further studies on the role of growth factors in flexor tendon healing in humans.
(Less)
- author
- Abrahamsson, S. O. LU ; Lundborg, G. LU and Lohmander, L. S.
- publishing date
- 1991-07
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Orthopaedic Research
- volume
- 9
- issue
- 4
- pages
- 8 pages
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- scopus:0026198752
- pmid:2045976
- ISSN
- 0736-0266
- language
- English
- LU publication?
- no
- id
- 29b86909-700a-46b1-8e80-13cafb6dba4b
- date added to LUP
- 2016-05-04 18:15:44
- date last changed
- 2024-02-18 17:07:47
@article{29b86909-700a-46b1-8e80-13cafb6dba4b, abstract = {{<p>Flexor tendons have an intrinsic ability for repair, with a capacity to metabolize matrix components and to proliferate. To identify factors with the potential of affecting those abilities, the effects of recombinant human insulin-like growth factor (rhIGF-I), insulin and fetal calf serum (FCS) on the synthesis of proteoglycan, collagen, and non-collagen protein and cell proliferation were investigated in short-term explant cultures of the deep flexor tendon of the rabbit. Matrix synthesis and cell proliferation were stimulated dose dependently by rhIGF-I at doses between 10 and 250 and at 10-100 ng/ml, respectively, by insulin at 250-5,000 ng/ml, and by FCS at 2-15%. Estimated maximal stimulation (E(max)) of up to three times the control value was observed with rhIGF-I at 250 ng/ml. Maximal stimulation was observed at 5,000 ng/ml with insulin, and FCS at 15%. rhIGF-I was more potent than insulin in stimulating protein synthesis and cell proliferation. The E(max) of stimulation of proteoglycan and collagen synthesis by rhIGF-I were two times that of FCS, and the E(max) of cell proliferation by FCS was twice that of rhIGF-I. Growth factors thus have the ability to stimulate matrix synthesis and cell proliferation in rabbit flexor tendon. This provides a rationale for further studies on the role of growth factors in flexor tendon healing in humans.</p>}}, author = {{Abrahamsson, S. O. and Lundborg, G. and Lohmander, L. S.}}, issn = {{0736-0266}}, language = {{eng}}, number = {{4}}, pages = {{495--502}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Journal of Orthopaedic Research}}, title = {{Recombinant human insulin-like growth factor-I stimulates in vitro matrix synthesis and cell proliferation in rabbit flexor tendon}}, volume = {{9}}, year = {{1991}}, }