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Strengths and weaknesses of the aniline-blue method used to test mushroom (1→3)-β-D-glucans obtained by microwave-assisted extractions

Gil-Ramírez, Alicia LU ; Smiderle, Fhernanda Ribeiro; Morales, Diego; Iacomini, Marcello and Soler-Rivas, Cristina (2019) In Carbohydrate Polymers 217. p.135-143
Abstract


The parameters to extract polysaccharide-enriched fractions (PEF) from mushrooms using MAE (microwave-assisted extraction) were adjusted following a full factorial 3
2
experimental design. The highest yield and total carbohydrate values, using Lentinula edodes as model mushroom, were obtained at 180 °C and 30 min. Several mushroom species were submitted to MAE and their PEF yields ranged between 12.1–44.2%. (1→3)-β-Glucans determination using a conventional fluorimetric method changed depending on the standard utilized. NMR analyses of PEF... (More)


The parameters to extract polysaccharide-enriched fractions (PEF) from mushrooms using MAE (microwave-assisted extraction) were adjusted following a full factorial 3
2
experimental design. The highest yield and total carbohydrate values, using Lentinula edodes as model mushroom, were obtained at 180 °C and 30 min. Several mushroom species were submitted to MAE and their PEF yields ranged between 12.1–44.2%. (1→3)-β-Glucans determination using a conventional fluorimetric method changed depending on the standard utilized. NMR analyses of PEF indicated that the presence of other polysaccharides in the extracts or their specific folding, might impair the proper determination of (1→3) linkages by the fluorophore. Mushrooms from Cantharellales order contained (1→3)-β-glucans but they were not detected with the fluorimetric method. Therefore, although the method (after adjustments) was sensitive enough to detect their presence in many mushroom extracts, it cannot be used for all species and it is also not recommended for quantitative determinations.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
(1→3)-β-D-glucans, Fluorimetric assay, MAE, Microwave-assisted extraction, Mushrooms, Polysaccharides
in
Carbohydrate Polymers
volume
217
pages
9 pages
publisher
Elsevier
external identifiers
  • scopus:85064509742
ISSN
0144-8617
DOI
10.1016/j.carbpol.2019.04.051
language
English
LU publication?
yes
id
2a1c39e7-08c2-43db-afaa-ac48cb09f3ff
date added to LUP
2019-05-02 14:36:09
date last changed
2019-08-14 04:35:41
@article{2a1c39e7-08c2-43db-afaa-ac48cb09f3ff,
  abstract     = {<p><br>
                                                         The parameters to extract polysaccharide-enriched fractions (PEF) from mushrooms using MAE (microwave-assisted extraction) were adjusted following a full factorial 3                             <br>
                            <sup>2</sup><br>
                                                          experimental design. The highest yield and total carbohydrate values, using Lentinula edodes as model mushroom, were obtained at 180 °C and 30 min. Several mushroom species were submitted to MAE and their PEF yields ranged between 12.1–44.2%. (1→3)-β-Glucans determination using a conventional fluorimetric method changed depending on the standard utilized. NMR analyses of PEF indicated that the presence of other polysaccharides in the extracts or their specific folding, might impair the proper determination of (1→3) linkages by the fluorophore. Mushrooms from Cantharellales order contained (1→3)-β-glucans but they were not detected with the fluorimetric method. Therefore, although the method (after adjustments) was sensitive enough to detect their presence in many mushroom extracts, it cannot be used for all species and it is also not recommended for quantitative determinations.                         <br>
                        </p>},
  author       = {Gil-Ramírez, Alicia and Smiderle, Fhernanda Ribeiro and Morales, Diego and Iacomini, Marcello and Soler-Rivas, Cristina},
  issn         = {0144-8617},
  keyword      = {(1→3)-β-D-glucans,Fluorimetric assay,MAE,Microwave-assisted extraction,Mushrooms,Polysaccharides},
  language     = {eng},
  pages        = {135--143},
  publisher    = {Elsevier},
  series       = {Carbohydrate Polymers},
  title        = {Strengths and weaknesses of the aniline-blue method used to test mushroom (1→3)-β-D-glucans obtained by microwave-assisted extractions},
  url          = {http://dx.doi.org/10.1016/j.carbpol.2019.04.051},
  volume       = {217},
  year         = {2019},
}