Evaluating Amyloid-beta Oligomers in Cerebrospinal Fluid as a Biomarker for Alzheimer's Disease
(2013) In PLoS ONE 8(6).- Abstract
- The current study evaluated amyloid-beta oligomers (A beta o) in cerebrospinal fluid as a clinical biomarker for Alzheimer's disease (AD). We developed a highly sensitive A beta o ELISA using the same N-terminal monoclonal antibody (82E1) for capture and detection. CSF samples from patients with AD, mild cognitive impairment (MCI), and healthy controls were examined. The assay was specific for oligomerized A beta with a lower limit of quantification of 200 fg/ml, and the assay signal showed a tight correlation with synthetic A beta o levels. Three clinical materials of well characterized AD patients (n = 199) and cognitively healthy controls (n = 148) from different clinical centers were included, together with a clinical material of... (More)
- The current study evaluated amyloid-beta oligomers (A beta o) in cerebrospinal fluid as a clinical biomarker for Alzheimer's disease (AD). We developed a highly sensitive A beta o ELISA using the same N-terminal monoclonal antibody (82E1) for capture and detection. CSF samples from patients with AD, mild cognitive impairment (MCI), and healthy controls were examined. The assay was specific for oligomerized A beta with a lower limit of quantification of 200 fg/ml, and the assay signal showed a tight correlation with synthetic A beta o levels. Three clinical materials of well characterized AD patients (n = 199) and cognitively healthy controls (n = 148) from different clinical centers were included, together with a clinical material of patients with MCI (n = 165). A beta o levels were elevated in the all three AD-control comparisons although with a large overlap and a separation from controls that was far from complete. Patients with MCI who later converted to AD had increased A beta o levels on a group level but several samples had undetectable levels. These results indicate that presence of high or measurable A beta o levels in CSF is clearly associated with AD, but the overlap is too large for the test to have any diagnostic potential on its own. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3987368
- author
- Holtta, Mikko ; Hansson, Oskar LU ; Andreasson, Ulf ; Hertze, Joakim LU ; Minthon, Lennart LU ; Nägga, Katarina LU ; Andreasen, Niels ; Zetterberg, Henrik and Blennow, Kaj
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- in
- PLoS ONE
- volume
- 8
- issue
- 6
- article number
- e66381
- publisher
- Public Library of Science (PLoS)
- external identifiers
-
- wos:000320363300078
- scopus:84879149021
- pmid:23799095
- ISSN
- 1932-6203
- DOI
- 10.1371/journal.pone.0066381
- language
- English
- LU publication?
- yes
- id
- 2a6855ac-7076-4538-8199-497af777db2f (old id 3987368)
- date added to LUP
- 2016-04-01 13:22:18
- date last changed
- 2022-05-15 04:50:50
@article{2a6855ac-7076-4538-8199-497af777db2f, abstract = {{The current study evaluated amyloid-beta oligomers (A beta o) in cerebrospinal fluid as a clinical biomarker for Alzheimer's disease (AD). We developed a highly sensitive A beta o ELISA using the same N-terminal monoclonal antibody (82E1) for capture and detection. CSF samples from patients with AD, mild cognitive impairment (MCI), and healthy controls were examined. The assay was specific for oligomerized A beta with a lower limit of quantification of 200 fg/ml, and the assay signal showed a tight correlation with synthetic A beta o levels. Three clinical materials of well characterized AD patients (n = 199) and cognitively healthy controls (n = 148) from different clinical centers were included, together with a clinical material of patients with MCI (n = 165). A beta o levels were elevated in the all three AD-control comparisons although with a large overlap and a separation from controls that was far from complete. Patients with MCI who later converted to AD had increased A beta o levels on a group level but several samples had undetectable levels. These results indicate that presence of high or measurable A beta o levels in CSF is clearly associated with AD, but the overlap is too large for the test to have any diagnostic potential on its own.}}, author = {{Holtta, Mikko and Hansson, Oskar and Andreasson, Ulf and Hertze, Joakim and Minthon, Lennart and Nägga, Katarina and Andreasen, Niels and Zetterberg, Henrik and Blennow, Kaj}}, issn = {{1932-6203}}, language = {{eng}}, number = {{6}}, publisher = {{Public Library of Science (PLoS)}}, series = {{PLoS ONE}}, title = {{Evaluating Amyloid-beta Oligomers in Cerebrospinal Fluid as a Biomarker for Alzheimer's Disease}}, url = {{https://lup.lub.lu.se/search/files/3331726/4362372.pdf}}, doi = {{10.1371/journal.pone.0066381}}, volume = {{8}}, year = {{2013}}, }