Protein-polyelectrolyte cluster formatio and redissolution: A Monte Carlo study
(2003) In Journal of the American Chemical Society 125(10). p.3140-3149- Abstract
- Aqueous solutions of proteins and oppositely charged polyelectrolytes were studied at different polyelectrolyte chain length, ionic strength, and protein-protein interaction potential as a function of the polyelectrolyte concentration. One of the protein models used represented lysozyme in aqueous environment. The model systems were solved by Monte Carlo simulations, and their properties were analyzed in terms of radial distribution functions, structure factors, and cluster composition probabilities. In the system with the strongest electrostatic protein-polyelectrolyte interaction the largest clusters were formed near or at equivalent amount of net protein charge and polyelectrolyte charge, whereas in excess of polyelectrolyte a... (More)
- Aqueous solutions of proteins and oppositely charged polyelectrolytes were studied at different polyelectrolyte chain length, ionic strength, and protein-protein interaction potential as a function of the polyelectrolyte concentration. One of the protein models used represented lysozyme in aqueous environment. The model systems were solved by Monte Carlo simulations, and their properties were analyzed in terms of radial distribution functions, structure factors, and cluster composition probabilities. In the system with the strongest electrostatic protein-polyelectrolyte interaction the largest clusters were formed near or at equivalent amount of net protein charge and polyelectrolyte charge, whereas in excess of polyelectrolyte a redissolution appeared. Shorter polyelectrolyte chains and increased ionic strength lead to weaker cluster formation. An inclusion of nonelectrostatic
protein-protein attraction promoted the protein-polyelectrolyte cluster
formation. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/121960
- author
- Carlsson, F ; Malmsten, M and Linse, Per LU
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of the American Chemical Society
- volume
- 125
- issue
- 10
- pages
- 3140 - 3149
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000181409500062
- pmid:12617682
- scopus:0037433601
- ISSN
- 1520-5126
- DOI
- 10.1021/ja020935a
- language
- English
- LU publication?
- yes
- id
- 2acc3245-85f6-48a0-81bd-2cf2a71f85d7 (old id 121960)
- date added to LUP
- 2016-04-01 16:39:42
- date last changed
- 2022-01-28 21:12:20
@article{2acc3245-85f6-48a0-81bd-2cf2a71f85d7,
abstract = {{Aqueous solutions of proteins and oppositely charged polyelectrolytes were studied at different polyelectrolyte chain length, ionic strength, and protein-protein interaction potential as a function of the polyelectrolyte concentration. One of the protein models used represented lysozyme in aqueous environment. The model systems were solved by Monte Carlo simulations, and their properties were analyzed in terms of radial distribution functions, structure factors, and cluster composition probabilities. In the system with the strongest electrostatic protein-polyelectrolyte interaction the largest clusters were formed near or at equivalent amount of net protein charge and polyelectrolyte charge, whereas in excess of polyelectrolyte a redissolution appeared. Shorter polyelectrolyte chains and increased ionic strength lead to weaker cluster formation. An inclusion of nonelectrostatic<br/><br>
protein-protein attraction promoted the protein-polyelectrolyte cluster<br/><br>
formation.}},
author = {{Carlsson, F and Malmsten, M and Linse, Per}},
issn = {{1520-5126}},
language = {{eng}},
number = {{10}},
pages = {{3140--3149}},
publisher = {{The American Chemical Society (ACS)}},
series = {{Journal of the American Chemical Society}},
title = {{Protein-polyelectrolyte cluster formatio and redissolution: A Monte Carlo study}},
url = {{http://dx.doi.org/10.1021/ja020935a}},
doi = {{10.1021/ja020935a}},
volume = {{125}},
year = {{2003}},
}