Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Distinct Cholesterol Localization in Glioblastoma Multiforme Revealed by Mass Spectrometry Imaging

Philipsen, Mai H. LU ; Hansson, Ellinor ; Manaprasertsak, Auraya LU ; Lange, Stefan ; Jennische, Eva ; Carén, Helena LU ; Gatzinsky, Kliment ; Jakola, Asgeir ; Hammarlund, Emma U. LU and Malmberg, Per LU (2023) In ACS Chemical Neuroscience 14(9). p.1602-1609
Abstract

Glioblastoma multiforme (GBM) is the most common and aggressive brain tumor in adults and is highly resistant to chemo- and radiotherapies. GBM has been associated with alterations in lipid contents, but lipid metabolism reprogramming in tumor cells is not fully elucidated. One of the key hurdles is to localize the lipid species that are correlated with tumor growth and invasion. A better understanding of the localization of abnormal lipid metabolism and its vulnerabilities may open up to novel therapeutic approaches. Here, we use time-of-flight secondary ion mass spectrometry (ToF-SIMS) to spatially probe the lipid composition in a GBM biopsy from two regions with different histopathologies: one region with most cells of uniform size... (More)

Glioblastoma multiforme (GBM) is the most common and aggressive brain tumor in adults and is highly resistant to chemo- and radiotherapies. GBM has been associated with alterations in lipid contents, but lipid metabolism reprogramming in tumor cells is not fully elucidated. One of the key hurdles is to localize the lipid species that are correlated with tumor growth and invasion. A better understanding of the localization of abnormal lipid metabolism and its vulnerabilities may open up to novel therapeutic approaches. Here, we use time-of-flight secondary ion mass spectrometry (ToF-SIMS) to spatially probe the lipid composition in a GBM biopsy from two regions with different histopathologies: one region with most cells of uniform size and shape, the homogeneous part, and the other with cells showing a great variation in size and shape, the heterogeneous part. Our results reveal elevated levels of cholesterol, diacylglycerols, and some phosphatidylethanolamine in the homogeneous part, while the heterogeneous part was dominated by a variety of fatty acids, phosphatidylcholine, and phosphatidylinositol species. We also observed a high expression of cholesterol in the homogeneous tumor region to be associated with large cells but not with macrophages. Our findings suggest that ToF-SIMS can distinguish in lipid distribution between parts within a human GBM tumor, which can be linked to different molecular mechanisms.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
cholesterol, glioblastoma, lipids, mass spectrometry imaging
in
ACS Chemical Neuroscience
volume
14
issue
9
pages
8 pages
publisher
The American Chemical Society (ACS)
external identifiers
  • pmid:37040529
  • scopus:85152687358
ISSN
1948-7193
DOI
10.1021/acschemneuro.2c00776
language
English
LU publication?
yes
id
2b541d35-6454-4218-ada3-fa7c40db3df3
date added to LUP
2023-07-21 12:31:33
date last changed
2024-04-19 23:54:06
@article{2b541d35-6454-4218-ada3-fa7c40db3df3,
  abstract     = {{<p>Glioblastoma multiforme (GBM) is the most common and aggressive brain tumor in adults and is highly resistant to chemo- and radiotherapies. GBM has been associated with alterations in lipid contents, but lipid metabolism reprogramming in tumor cells is not fully elucidated. One of the key hurdles is to localize the lipid species that are correlated with tumor growth and invasion. A better understanding of the localization of abnormal lipid metabolism and its vulnerabilities may open up to novel therapeutic approaches. Here, we use time-of-flight secondary ion mass spectrometry (ToF-SIMS) to spatially probe the lipid composition in a GBM biopsy from two regions with different histopathologies: one region with most cells of uniform size and shape, the homogeneous part, and the other with cells showing a great variation in size and shape, the heterogeneous part. Our results reveal elevated levels of cholesterol, diacylglycerols, and some phosphatidylethanolamine in the homogeneous part, while the heterogeneous part was dominated by a variety of fatty acids, phosphatidylcholine, and phosphatidylinositol species. We also observed a high expression of cholesterol in the homogeneous tumor region to be associated with large cells but not with macrophages. Our findings suggest that ToF-SIMS can distinguish in lipid distribution between parts within a human GBM tumor, which can be linked to different molecular mechanisms.</p>}},
  author       = {{Philipsen, Mai H. and Hansson, Ellinor and Manaprasertsak, Auraya and Lange, Stefan and Jennische, Eva and Carén, Helena and Gatzinsky, Kliment and Jakola, Asgeir and Hammarlund, Emma U. and Malmberg, Per}},
  issn         = {{1948-7193}},
  keywords     = {{cholesterol; glioblastoma; lipids; mass spectrometry imaging}},
  language     = {{eng}},
  number       = {{9}},
  pages        = {{1602--1609}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{ACS Chemical Neuroscience}},
  title        = {{Distinct Cholesterol Localization in Glioblastoma Multiforme Revealed by Mass Spectrometry Imaging}},
  url          = {{http://dx.doi.org/10.1021/acschemneuro.2c00776}},
  doi          = {{10.1021/acschemneuro.2c00776}},
  volume       = {{14}},
  year         = {{2023}},
}