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Leucocyte recruitment and molecular fortification of keratinocytes triggered by streptococcal M1 protein

Persson, Sandra T. LU ; Hauri, Simon LU ; Malmström, Johan LU and Herwald, Heiko LU (2018) In Cellular Microbiology 20(1).
Abstract

Streptococcus pyogenes of the M1 serotype is commonly associated with invasive streptococcal infections and development of streptococcal toxic shock syndrome. The M1 protein is a powerful inducer of inflammatory responses for several human cell types, but the reason why M1 protein-related strains is over-represented in invasive streptococcal diseases is still not understood. This study was undertaken to investigate if soluble M1 protein can aggravate the severity of streptococcal skin infections in respect to inflammation, leucocyte recruitment, and tissue remodelling as seen in patients with cellulitis and necrotizing fasciitis. We found that HaCaT cells are able to recruit activated leucocytes when encountering M1 protein. Neither the... (More)

Streptococcus pyogenes of the M1 serotype is commonly associated with invasive streptococcal infections and development of streptococcal toxic shock syndrome. The M1 protein is a powerful inducer of inflammatory responses for several human cell types, but the reason why M1 protein-related strains is over-represented in invasive streptococcal diseases is still not understood. This study was undertaken to investigate if soluble M1 protein can aggravate the severity of streptococcal skin infections in respect to inflammation, leucocyte recruitment, and tissue remodelling as seen in patients with cellulitis and necrotizing fasciitis. We found that HaCaT cells are able to recruit activated leucocytes when encountering M1 protein. Neither the bacterial protein nor activated leucocytes caused cell damage on HaCaT cells, instead HaCaT cells responded to the bacterial virulence factor by releasing several proteins protective against bacterial infection and leucocyte responses. However, although not cytotoxic, M1 protein completely abolished wound healing abilities of HaCaT cells. Taken together, our results demonstrate that M1 protein is a critical virulence factor that can augment streptococcal skin infection suggesting that the protein is an interesting target for drug development.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Immunology, Infection, Microbial-cell interaction, Streptococci, Stress response, Virulence
in
Cellular Microbiology
volume
20
issue
1
publisher
Wiley-Blackwell
external identifiers
  • scopus:85031738987
  • wos:000417933800005
ISSN
1462-5814
DOI
10.1111/cmi.12792
language
English
LU publication?
yes
id
2b5df078-5607-4fe5-85cc-7281f3a44769
date added to LUP
2017-10-31 09:22:18
date last changed
2018-05-27 04:47:09
@article{2b5df078-5607-4fe5-85cc-7281f3a44769,
  abstract     = {<p>Streptococcus pyogenes of the M1 serotype is commonly associated with invasive streptococcal infections and development of streptococcal toxic shock syndrome. The M1 protein is a powerful inducer of inflammatory responses for several human cell types, but the reason why M1 protein-related strains is over-represented in invasive streptococcal diseases is still not understood. This study was undertaken to investigate if soluble M1 protein can aggravate the severity of streptococcal skin infections in respect to inflammation, leucocyte recruitment, and tissue remodelling as seen in patients with cellulitis and necrotizing fasciitis. We found that HaCaT cells are able to recruit activated leucocytes when encountering M1 protein. Neither the bacterial protein nor activated leucocytes caused cell damage on HaCaT cells, instead HaCaT cells responded to the bacterial virulence factor by releasing several proteins protective against bacterial infection and leucocyte responses. However, although not cytotoxic, M1 protein completely abolished wound healing abilities of HaCaT cells. Taken together, our results demonstrate that M1 protein is a critical virulence factor that can augment streptococcal skin infection suggesting that the protein is an interesting target for drug development.</p>},
  articleno    = {e12792},
  author       = {Persson, Sandra T. and Hauri, Simon and Malmström, Johan and Herwald, Heiko},
  issn         = {1462-5814},
  keyword      = {Immunology,Infection,Microbial-cell interaction,Streptococci,Stress response,Virulence},
  language     = {eng},
  number       = {1},
  publisher    = {Wiley-Blackwell},
  series       = {Cellular Microbiology},
  title        = {Leucocyte recruitment and molecular fortification of keratinocytes triggered by streptococcal M1 protein},
  url          = {http://dx.doi.org/10.1111/cmi.12792},
  volume       = {20},
  year         = {2018},
}