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Influence of salinomycin treatment on division and movement of individual cancer cells cultured in normoxia or hypoxia evaluated with time-lapse digital holographic microscopy

Kamlund, Sofia LU ; Strand, Daniel LU ; Janicke, Birgit LU ; Alm, Kersti LU and Oredsson, Stina LU (2017) In Cell Cycle
Abstract

Most studies on new cancer drugs are based on population-derived data, where the absence of response of a small population may pass unnoticed. Thus, individual longitudinal tracking of cells is important for the future development of efficient cancer treatments. We have used digital holographic microscopy to track individual JIMT-1 human breast cancer cells and L929 mouse fibroblast cultivated in normoxia or hypoxia. In addition, JIMT-1 cells were treated with salinomycin, a cancer stem cell targeting compound. Three-day time-lapse movies were captured and individual cells were analysed with respect to cell division (cell cycle length) and cell movement. Comparing population-doubling time derived from population-based growth curves and... (More)

Most studies on new cancer drugs are based on population-derived data, where the absence of response of a small population may pass unnoticed. Thus, individual longitudinal tracking of cells is important for the future development of efficient cancer treatments. We have used digital holographic microscopy to track individual JIMT-1 human breast cancer cells and L929 mouse fibroblast cultivated in normoxia or hypoxia. In addition, JIMT-1 cells were treated with salinomycin, a cancer stem cell targeting compound. Three-day time-lapse movies were captured and individual cells were analysed with respect to cell division (cell cycle length) and cell movement. Comparing population-doubling time derived from population-based growth curves and individual cell cycle time data from time-lapse movies show that the former hide a sub-population of dividing cells. Salinomycin treatment increased the motility of cells, however, this motility did not result in an increased distant migration i.e. the cells increased their local movement. MCF-7 breast cancer cells showed similar motility behaviour as salinomycin-treated JIMT-1 cells. We suggest that combining features, such as motility and migration, can be used to distinguish cancer cells with mesenchymal (JIMT-1) and epithelial (MCF-7) features. The data clearly emphasize the importance of longitudinal cell tracking to understand the biology of individual cells under different conditions.

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author
organization
publishing date
type
Contribution to journal
publication status
epub
subject
keywords
cell cycle, cell migration, Digital holography, hypoxia, longitudinal tracking of individual cells, normoxia, salinomycin, time-lapse
in
Cell Cycle
pages
11 pages
publisher
Landes Bioscience
external identifiers
  • scopus:85030540468
ISSN
1538-4101
DOI
10.1080/15384101.2017.1380131
language
English
LU publication?
yes
id
2b81b656-f709-4344-a17e-4bc96b7aa7b0
date added to LUP
2017-10-16 10:52:37
date last changed
2018-01-16 03:00:13
@article{2b81b656-f709-4344-a17e-4bc96b7aa7b0,
  abstract     = {<p>Most studies on new cancer drugs are based on population-derived data, where the absence of response of a small population may pass unnoticed. Thus, individual longitudinal tracking of cells is important for the future development of efficient cancer treatments. We have used digital holographic microscopy to track individual JIMT-1 human breast cancer cells and L929 mouse fibroblast cultivated in normoxia or hypoxia. In addition, JIMT-1 cells were treated with salinomycin, a cancer stem cell targeting compound. Three-day time-lapse movies were captured and individual cells were analysed with respect to cell division (cell cycle length) and cell movement. Comparing population-doubling time derived from population-based growth curves and individual cell cycle time data from time-lapse movies show that the former hide a sub-population of dividing cells. Salinomycin treatment increased the motility of cells, however, this motility did not result in an increased distant migration i.e. the cells increased their local movement. MCF-7 breast cancer cells showed similar motility behaviour as salinomycin-treated JIMT-1 cells. We suggest that combining features, such as motility and migration, can be used to distinguish cancer cells with mesenchymal (JIMT-1) and epithelial (MCF-7) features. The data clearly emphasize the importance of longitudinal cell tracking to understand the biology of individual cells under different conditions.</p>},
  author       = {Kamlund, Sofia and Strand, Daniel and Janicke, Birgit and Alm, Kersti and Oredsson, Stina},
  issn         = {1538-4101},
  keyword      = {cell cycle,cell migration,Digital holography,hypoxia,longitudinal tracking of individual cells,normoxia,salinomycin,time-lapse},
  language     = {eng},
  month        = {10},
  pages        = {11},
  publisher    = {Landes Bioscience},
  series       = {Cell Cycle},
  title        = {Influence of salinomycin treatment on division and movement of individual cancer cells cultured in normoxia or hypoxia evaluated with time-lapse digital holographic microscopy},
  url          = {http://dx.doi.org/10.1080/15384101.2017.1380131},
  year         = {2017},
}